Long established as a powerful tool for determining the structure of small molecules, nuclear magnetic resonance (NMR) spectroscopy is now unravelling the secrets of previously inaccessible biological macromolecules, thanks to recent advances in the field. Bigger and more powerful spectrometers with higher magnetic field strengths offer new insights into the reactions happening in our bodies.
Enzymes, like other proteins, were traditionally characterised in the solid state by X-ray crystallography. NMR has a significant advantage as biomolecules can now be studied in their natural environment in bodily fluids and even in cell membranes using solid state NMR.
Protein-digesting enzymes called proteases play a role in propagating the AIDS virus, in allowing cancers and parasites to move through tissues and in the production of the plaque protein which causes Alzheimer’s disease.
Inhibiting these enzymes is key to treating such diseases. Drugs are designed to target enzymes by slotting into their active sites and shutting them down. Drug design seeks to optimise inhibitor binding, so we need to understand how the inhibitors interact with an enzyme.
"We are synthesising protease inhibitors and using NMR to determine how they interact with specific proteases. By studying these interactions we hope to see ways of optimising an inhibitor’s ability to inhibit the specific protease involved in a given disease,” explains Professor Malthouse.
It is essential that potent protease inhibitors designed will only target the protease involved in the disease and not those which are essential for our bodies.
"We are currently starting to synthesise and characterise a range of inhibitors which we hope will provide important insights into the development of drugs to treat a range of medical conditions,” continues Professor Malthouse.
New targets for treatment of diabetes and obesity
A group including Professor Malthouse and Dr Chandralal Hewage, NMR scientist at the NMR Centre in UCD Conway Institute, have exploited NMR technology to solve the 3D solution structure of the gastrointestinal polypeptide GIP.
GIP is a hormone that stimulates the secretion of insulin after ingestion of food and has been linked to diabetes and obesity-related diseases.
A 3D picture of the protein was built step by step using a range of NMR experiments and molecular modelling calculations. Two-dimensional NMR spectra revealed information about the connectivities of the atoms, allowing the identity of each amino acid residue to be determined.
Dr Hewage explains the significance of these studies: “Understanding the structural requirements for the biological activity of GIP will help in the design of new drugs for diabetes and obesity related disorders.”
"Proteins are huge molecules but commercially viable drugs need to be a lot smaller both for ease of entry into cells and because of the manufacturing costs involved. Once the structure of the protein is known, the important residues can be identified and a smaller drug molecule synthesised.”
Orla Donoghue | alfa
20.11.2017 | Washington University in St. Louis
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The formation of stars in distant galaxies is still largely unexplored. For the first time, astron-omers at the University of Geneva have now been able to closely observe a star system six billion light-years away. In doing so, they are confirming earlier simulations made by the University of Zurich. One special effect is made possible by the multiple reflections of images that run through the cosmos like a snake.
Today, astronomers have a pretty accurate idea of how stars were formed in the recent cosmic past. But do these laws also apply to older galaxies? For around a...
Just because someone is smart and well-motivated doesn't mean he or she can learn the visual skills needed to excel at tasks like matching fingerprints, interpreting medical X-rays, keeping track of aircraft on radar displays or forensic face matching.
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Computer Tomography (CT) is a standard procedure in hospitals, but so far, the technology has not been suitable for imaging extremely small objects. In PNAS, a team from the Technical University of Munich (TUM) describes a Nano-CT device that creates three-dimensional x-ray images at resolutions up to 100 nanometers. The first test application: Together with colleagues from the University of Kassel and Helmholtz-Zentrum Geesthacht the researchers analyzed the locomotory system of a velvet worm.
During a CT analysis, the object under investigation is x-rayed and a detector measures the respective amount of radiation absorbed from various angles....
The quantum world is fragile; error correction codes are needed to protect the information stored in a quantum object from the deteriorating effects of noise. Quantum physicists in Innsbruck have developed a protocol to pass quantum information between differently encoded building blocks of a future quantum computer, such as processors and memories. Scientists may use this protocol in the future to build a data bus for quantum computers. The researchers have published their work in the journal Nature Communications.
Future quantum computers will be able to solve problems where conventional computers fail today. We are still far away from any large-scale implementation,...
Pillared graphene would transfer heat better if the theoretical material had a few asymmetric junctions that caused wrinkles, according to Rice University...
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