Forum for Science, Industry and Business

Sponsored by:     3M 
Search our Site:

 

Ghost protein leaves fresh tracks in the cell

01.11.2006
Spectrin and ankyrin are two essential proteins acting like bricks and mortar to shape and fortify cell membranes. But distinguishing which protein is the brick and which is the mortar has turned out to be difficult. New evidence suggests that spectrin can do both jobs at once.

Ron Dubreuil, associate professor of biological sciences at the University of Illinois at Chicago, reports the finding in the Oct. 23 issue of the Journal of Cell Biology.

Spectrin was first discovered in red blood cells, where it forms a protein scaffold under the cell's membrane. It was named for its ability to maintain the shape of cell "ghosts," which have been emptied of their contents. Ankyrin serves as the mortar that attaches spectrin to the red blood cell membrane.

Dubreuil and his UIC co-workers have spent a decade looking at different types of cells -- mostly epithelial -- trying to learn what cues tell spectrin where to assemble in cells. They use the fruit fly as their test animal because its genetic makeup has many striking similarities to humans.

... more about:
»Dubreuil »Membrane »ankyrin »spectrin

"In our study, we showed spectrin doesn't have to bind to ankyrin to do its job," said Dubreuil. "This hints at a complexity we never had any idea about in trying to understand how these molecules work."

Dubreuil and his colleagues initially assumed that ankyrin was the key to targeting spectrin in all cells. But research in many laboratories had failed to find a cue for targeting that acted through ankyrin, so Dubreuil reworked his hypothesis.

"We decided to throw out our assumptions and start fresh," he said.

A laboratory fly was genetically engineered so that spectrin could no longer bind to ankyrin -- which, Dubreuil assumed, meant that spectrin should no longer attach to the membrane.

"We thought that was going to kill the function of the protein," he said, "but it didn't affect the ability of the protein to reach its destination at all. The molecule targeted correctly to the cell membrane." In fact, the genetically engineered flies often survived to adulthood, while mutants that lacked spectrin altogether died very early in development.

Meanwhile, Dubreuil discovered that another region of spectrin, called the PH domain, unexpectedly played an critical role. Removing the PH domain left spectrin unable to bind to the membrane in certain cells, and those flies died.

Dubreuil's research seeks to clarify how these proteins function in different cells. The hope is that researchers may one day create therapeutic molecules to compensate for genetic lesions in diseases such as hereditary anemia, Duchenne muscular dystrophy, cardiac arrhythmia and the degenerative brain disease spinocerebellar ataxia 5.

"As we learn more about mutations involving spectrin and their relationships to human diseases, we're going to have more and more questions about how these mutations affect specific functions of the molecule," he said.

Paul Francuch | EurekAlert!
Further information:
http://www.uic.edu

Further reports about: Dubreuil Membrane ankyrin spectrin

More articles from Life Sciences:

nachricht Ion treatments for cardiac arrhythmia — Non-invasive alternative to catheter-based surgery
20.01.2017 | GSI Helmholtzzentrum für Schwerionenforschung GmbH

nachricht Seeking structure with metagenome sequences
20.01.2017 | DOE/Joint Genome Institute

All articles from Life Sciences >>>

The most recent press releases about innovation >>>

Die letzten 5 Focus-News des innovations-reports im Überblick:

Im Focus: Traffic jam in empty space

New success for Konstanz physicists in studying the quantum vacuum

An important step towards a completely new experimental access to quantum physics has been made at University of Konstanz. The team of scientists headed by...

Im Focus: How gut bacteria can make us ill

HZI researchers decipher infection mechanisms of Yersinia and immune responses of the host

Yersiniae cause severe intestinal infections. Studies using Yersinia pseudotuberculosis as a model organism aim to elucidate the infection mechanisms of these...

Im Focus: Interfacial Superconductivity: Magnetic and superconducting order revealed simultaneously

Researchers from the University of Hamburg in Germany, in collaboration with colleagues from the University of Aarhus in Denmark, have synthesized a new superconducting material by growing a few layers of an antiferromagnetic transition-metal chalcogenide on a bismuth-based topological insulator, both being non-superconducting materials.

While superconductivity and magnetism are generally believed to be mutually exclusive, surprisingly, in this new material, superconducting correlations...

Im Focus: Studying fundamental particles in materials

Laser-driving of semimetals allows creating novel quasiparticle states within condensed matter systems and switching between different states on ultrafast time scales

Studying properties of fundamental particles in condensed matter systems is a promising approach to quantum field theory. Quasiparticles offer the opportunity...

Im Focus: Designing Architecture with Solar Building Envelopes

Among the general public, solar thermal energy is currently associated with dark blue, rectangular collectors on building roofs. Technologies are needed for aesthetically high quality architecture which offer the architect more room for manoeuvre when it comes to low- and plus-energy buildings. With the “ArKol” project, researchers at Fraunhofer ISE together with partners are currently developing two façade collectors for solar thermal energy generation, which permit a high degree of design flexibility: a strip collector for opaque façade sections and a solar thermal blind for transparent sections. The current state of the two developments will be presented at the BAU 2017 trade fair.

As part of the “ArKol – development of architecturally highly integrated façade collectors with heat pipes” project, Fraunhofer ISE together with its partners...

All Focus news of the innovation-report >>>

Anzeige

Anzeige

Event News

Sustainable Water use in Agriculture in Eastern Europe and Central Asia

19.01.2017 | Event News

12V, 48V, high-voltage – trends in E/E automotive architecture

10.01.2017 | Event News

2nd Conference on Non-Textual Information on 10 and 11 May 2017 in Hannover

09.01.2017 | Event News

 
Latest News

Helmholtz International Fellow Award for Sarah Amalia Teichmann

20.01.2017 | Awards Funding

An innovative high-performance material: biofibers made from green lacewing silk

20.01.2017 | Materials Sciences

Ion treatments for cardiac arrhythmia — Non-invasive alternative to catheter-based surgery

20.01.2017 | Life Sciences

VideoLinks
B2B-VideoLinks
More VideoLinks >>>