Forum for Science, Industry and Business

Sponsored by:     3M 
Search our Site:


Watching DNA Repair in Real Time

Direct observations of DNA are giving new insights into how genetic material is copied and repaired.

"We can monitor the process directly, and that gives us a different perspective," said Roberto Galletto, a postdoctoral scholar at UC Davis and first author on a paper published Sept. 20 on the Web site of the journal Nature.

In E. coli bacteria, molecules of an enzyme called RecA attach themselves along a DNA strand, stretching it out and forming a filament. A piece of complementary DNA lines up along side it, and pieces of DNA can be swapped in to repair gaps in the original strand. A similar protein, called Rad51, does the same job in humans.

"How RecA and Rad51 assemble into filaments determines the outcome of DNA repair, but very little is known about how assembly is controlled," said senior author Stephen Kowalczykowski, professor in the sections of Microbiology and of Molecular and Cellular Biology and director of the Center for Genetics and Development at UC Davis. Genes that control the human gene, Rad51, have been linked to increased risk of breast cancer.

... more about:
»DNA »Kowalczykowski »RAD51 »RecA

Galletto attached a short piece of DNA to a tiny latex bead and placed it in a flow chamber, held by laser beam "tweezers." Fluid flowing past made the DNA stream out like a banner. Then he nudged it into an adjacent channel containing fluorescently-tagged RecA. After short intervals of time, he moved it back to the first chamber to observe the results.

By repeatedly dipping the same piece of DNA into the fluorescent channel, the researchers could see the RecA form clusters of four to five molecules on the DNA. Once those clusters had formed, the DNA/RecA filament rapidly grew in both directions. The measurements made in those experiments will be the baseline for future studies of both RecA and Rad51, Kowalczykowski said.

The new work adapts an approach developed by Kowalczykowski and Ronald J. Baskin, professor of molecular and cellular biology, to study single enzymes at work unwinding DNA strands. That research was first published in Nature in 2001.

In addition to Galletto, Kowalczykowski and Baskin, the research team included postdoctoral scholar Ichiro Amitani. The work was funded by the National Institutes of Health and a fellowship awarded to Galletto by the Jeane B. Kempner Foundation.

Andy Fell | EurekAlert!
Further information:

Further reports about: DNA Kowalczykowski RAD51 RecA

More articles from Life Sciences:

nachricht Novel mechanisms of action discovered for the skin cancer medication Imiquimod
21.10.2016 | Technische Universität München

nachricht Second research flight into zero gravity
21.10.2016 | Universität Zürich

All articles from Life Sciences >>>

The most recent press releases about innovation >>>

Die letzten 5 Focus-News des innovations-reports im Überblick:

Im Focus: New 3-D wiring technique brings scalable quantum computers closer to reality

Researchers from the Institute for Quantum Computing (IQC) at the University of Waterloo led the development of a new extensible wiring technique capable of controlling superconducting quantum bits, representing a significant step towards to the realization of a scalable quantum computer.

"The quantum socket is a wiring method that uses three-dimensional wires based on spring-loaded pins to address individual qubits," said Jeremy Béjanin, a PhD...

Im Focus: Scientists develop a semiconductor nanocomposite material that moves in response to light

In a paper in Scientific Reports, a research team at Worcester Polytechnic Institute describes a novel light-activated phenomenon that could become the basis for applications as diverse as microscopic robotic grippers and more efficient solar cells.

A research team at Worcester Polytechnic Institute (WPI) has developed a revolutionary, light-activated semiconductor nanocomposite material that can be used...

Im Focus: Diamonds aren't forever: Sandia, Harvard team create first quantum computer bridge

By forcefully embedding two silicon atoms in a diamond matrix, Sandia researchers have demonstrated for the first time on a single chip all the components needed to create a quantum bridge to link quantum computers together.

"People have already built small quantum computers," says Sandia researcher Ryan Camacho. "Maybe the first useful one won't be a single giant quantum computer...

Im Focus: New Products - Highlights of COMPAMED 2016

COMPAMED has become the leading international marketplace for suppliers of medical manufacturing. The trade fair, which takes place every November and is co-located to MEDICA in Dusseldorf, has been steadily growing over the past years and shows that medical technology remains a rapidly growing market.

In 2016, the joint pavilion by the IVAM Microtechnology Network, the Product Market “High-tech for Medical Devices”, will be located in Hall 8a again and will...

Im Focus: Ultra-thin ferroelectric material for next-generation electronics

'Ferroelectric' materials can switch between different states of electrical polarization in response to an external electric field. This flexibility means they show promise for many applications, for example in electronic devices and computer memory. Current ferroelectric materials are highly valued for their thermal and chemical stability and rapid electro-mechanical responses, but creating a material that is scalable down to the tiny sizes needed for technologies like silicon-based semiconductors (Si-based CMOS) has proven challenging.

Now, Hiroshi Funakubo and co-workers at the Tokyo Institute of Technology, in collaboration with researchers across Japan, have conducted experiments to...

All Focus news of the innovation-report >>>



Event News

#IC2S2: When Social Science meets Computer Science - GESIS will host the IC2S2 conference 2017

14.10.2016 | Event News

Agricultural Trade Developments and Potentials in Central Asia and the South Caucasus

14.10.2016 | Event News

World Health Summit – Day Three: A Call to Action

12.10.2016 | Event News

Latest News

Resolving the mystery of preeclampsia

21.10.2016 | Health and Medicine

Stanford researchers create new special-purpose computer that may someday save us billions

21.10.2016 | Information Technology

From ancient fossils to future cars

21.10.2016 | Materials Sciences

More VideoLinks >>>