Forum for Science, Industry and Business

Sponsored by:     3M 
Search our Site:

 

Gene variants reveal susceptibility to cardiovascular disease

28.08.2006
Variations in a gene that acts as a switch to turn on other genes may predispose individuals to heart disease, an international team of researchers led by Duke University Medical Center scientists has discovered.

Further study of this master switch -- a gene called GATA2 -- and the genes it controls may uncover a regulatory network that influences whether a person inherits coronary artery disease, the most common form of heart disease in the Western world, according to the researchers. The discovery also may lead to development of genetic tests to predict an individual's risk of developing coronary artery disease, the scientists said.

"We hope that one day it will be possible to use these gene variations to predict who is susceptible to cardiovascular disease," said Jessica J. Connelly, a postdoctoral fellow at the Duke Center for Human Genetics and lead author on the study. "This finding is the first step before we can develop such a test for use in patients."

People who know they are at higher risk may be encouraged to take early steps to modify behaviors, such as smoking or consuming foods high in saturated fats, that are known to play a role in promoting heart disease, the scientists said.

The team reports its findings in the August 2006 issue of Public Library of Science (PLoS) Genetics. The research was sponsored by the National Institutes of Health.

Coronary artery disease affects more than 13 million Americans and is one of the nation's leading causes of death. The disease occurs when the arteries supplying blood to the heart become narrowed or clogged by plaque deposits. Left untreated, the disease can completely block the blood flow to the heart, leading to a heart attack.

Coronary artery disease is what scientists call a "complex" genetic disease – that is, it results from the accumulation of a number of small genetic changes that influence an individual's ability to cope with environmental and biological effects. While risk factors such as smoking, high blood pressure and high cholesterol are known to contribute to coronary artery disease, little is known about genes that render an individual susceptible to developing the disease, according to the Duke researchers.

"It is extremely difficult to pin down genes associated with a complex disease such as heart disease," said Simon G. Gregory, Ph.D., assistant professor of medicine at the Duke Center for Human Genetics and senior investigator on the study.

Two pieces of evidence pointed the researchers' attention to GATA2 as a likely candidate, Gregory said.

In a previous study, the researchers had scanned the entire genome -- the body's genetic blueprint -- of a group of families with at least two siblings with early onset coronary artery disease, looking for regions of "linkage" where DNA variations appeared to be inherited along with the disease. They found just such a region: a small section of the long arm of chromosome 3 where GATA2 is located. Chromosome 3 is one of the 23 pairs of chromosomes that comprise the human genome.

In a separate study led by David Seo, M.D., assistant professor of medicine, another group of Duke researchers found that GATA2 was turned on, or expressed, differently in diseased areas of aorta, the primary artery supplying the heart, in people with damaged hearts. This finding suggested that the gene could be involved in susceptibility to coronary artery disease, the researcher said.

In the current study, the researchers focused on specific gene variants, called single nucleotide polymorphisms (SNPs), which occur when a single nucleotide building block in the long strand of DNA is altered. The researchers sought SNPs that occurred more or less often in individuals with coronary artery disease than in individuals without it, as such a link would indicate that these gene variants were associated with the disease.

The researchers obtained DNA from 3,000 individuals from 1,000 families affected by coronary artery disease, through a collaborative study, called GENECARD, led by William E. Kraus, M.D., associate professor of medicine, under way at the Duke Center for Human Genetics. Using these DNA samples, the researchers scanned the GATA2 gene for SNPs that differed in sequence between individuals with and without coronary heart disease.

"We identified five SNPs that were significantly associated with early onset coronary artery disease," Gregory said.

The researchers then looked for the same SNPs in a separate group of 600 patients with early onset coronary artery disease who had volunteered to be studied while being examined at the cardiac catheterization laboratories at Duke University Hospital. The team identified significant association of two of the same SNPs in this independent group of patients. This finding, according to the researchers, validated the suspected link between GATA2 and coronary artery disease.

"A huge strength of our study is that we used two separate populations, finding the association between GATA2 and coronary artery disease in one population and then validating the finding in another," Connelly said.

GATA2 is a transcription factor, a master switch that controls when and where other genes are expressed. According the researchers, the SNPs identified in this study may change the ability of this transcription factor to influence the activity of many other genes, demonstrating how small genetic changes can influence multiple genetic outcomes.

The researchers said they now can use molecular techniques to look at where GATA2 acts within the genome to see what other genes also contribute to cause cardiovascular disease.

"As science progresses in this field, we are compiling a portfolio of genes that contribute to cardiovascular disease," Gregory said.

The hope, according to the Duke team, is that this discovery will be followed by others that eventually will enable scientists to identify people who are predisposed to developing coronary artery disease long before they develop any symptoms of the disease.

"What we have found is that changes in GATA2 affect susceptibility to developing coronary artery disease," Connelly said. "Eventually, we hope to create a diagnostic test containing all the genes affected in cardiovascular disease and use it to identify which SNPs are present in an individual. This approach will enable us to generate a profile of risk for developing cardiovascular disease."

The profile would not say conclusively whether or not a person would develop coronary heart disease, but could tell individuals whether they are more or less at risk for developing the disease, Gregory said.

Marla Vacek Broadfoot | EurekAlert!
Further information:
http://www.duke.edu

More articles from Life Sciences:

nachricht Rainbow colors reveal cell history: Uncovering β-cell heterogeneity
22.09.2017 | DFG-Forschungszentrum für Regenerative Therapien TU Dresden

nachricht The pyrenoid is a carbon-fixing liquid droplet
22.09.2017 | Max-Planck-Institut für Biochemie

All articles from Life Sciences >>>

The most recent press releases about innovation >>>

Die letzten 5 Focus-News des innovations-reports im Überblick:

Im Focus: The pyrenoid is a carbon-fixing liquid droplet

Plants and algae use the enzyme Rubisco to fix carbon dioxide, removing it from the atmosphere and converting it into biomass. Algae have figured out a way to increase the efficiency of carbon fixation. They gather most of their Rubisco into a ball-shaped microcompartment called the pyrenoid, which they flood with a high local concentration of carbon dioxide. A team of scientists at Princeton University, the Carnegie Institution for Science, Stanford University and the Max Plank Institute of Biochemistry have unravelled the mysteries of how the pyrenoid is assembled. These insights can help to engineer crops that remove more carbon dioxide from the atmosphere while producing more food.

A warming planet

Im Focus: Highly precise wiring in the Cerebral Cortex

Our brains house extremely complex neuronal circuits, whose detailed structures are still largely unknown. This is especially true for the so-called cerebral cortex of mammals, where among other things vision, thoughts or spatial orientation are being computed. Here the rules by which nerve cells are connected to each other are only partly understood. A team of scientists around Moritz Helmstaedter at the Frankfiurt Max Planck Institute for Brain Research and Helene Schmidt (Humboldt University in Berlin) have now discovered a surprisingly precise nerve cell connectivity pattern in the part of the cerebral cortex that is responsible for orienting the individual animal or human in space.

The researchers report online in Nature (Schmidt et al., 2017. Axonal synapse sorting in medial entorhinal cortex, DOI: 10.1038/nature24005) that synapses in...

Im Focus: Tiny lasers from a gallery of whispers

New technique promises tunable laser devices

Whispering gallery mode (WGM) resonators are used to make tiny micro-lasers, sensors, switches, routers and other devices. These tiny structures rely on a...

Im Focus: Ultrafast snapshots of relaxing electrons in solids

Using ultrafast flashes of laser and x-ray radiation, scientists at the Max Planck Institute of Quantum Optics (Garching, Germany) took snapshots of the briefest electron motion inside a solid material to date. The electron motion lasted only 750 billionths of the billionth of a second before it fainted, setting a new record of human capability to capture ultrafast processes inside solids!

When x-rays shine onto solid materials or large molecules, an electron is pushed away from its original place near the nucleus of the atom, leaving a hole...

Im Focus: Quantum Sensors Decipher Magnetic Ordering in a New Semiconducting Material

For the first time, physicists have successfully imaged spiral magnetic ordering in a multiferroic material. These materials are considered highly promising candidates for future data storage media. The researchers were able to prove their findings using unique quantum sensors that were developed at Basel University and that can analyze electromagnetic fields on the nanometer scale. The results – obtained by scientists from the University of Basel’s Department of Physics, the Swiss Nanoscience Institute, the University of Montpellier and several laboratories from University Paris-Saclay – were recently published in the journal Nature.

Multiferroics are materials that simultaneously react to electric and magnetic fields. These two properties are rarely found together, and their combined...

All Focus news of the innovation-report >>>

Anzeige

Anzeige

Event News

“Lasers in Composites Symposium” in Aachen – from Science to Application

19.09.2017 | Event News

I-ESA 2018 – Call for Papers

12.09.2017 | Event News

EMBO at Basel Life, a new conference on current and emerging life science research

06.09.2017 | Event News

 
Latest News

Rainbow colors reveal cell history: Uncovering β-cell heterogeneity

22.09.2017 | Life Sciences

Penn first in world to treat patient with new radiation technology

22.09.2017 | Medical Engineering

Calculating quietness

22.09.2017 | Physics and Astronomy

VideoLinks
B2B-VideoLinks
More VideoLinks >>>