Forum for Science, Industry and Business

Sponsored by:     3M 
Search our Site:

 

Gluing Cells - Precise sticking of living cells on carriers by microelectrochemical methods

03.08.2006
The adhesion and growth of cells on solid carriers is required for many applications. Laboratory-cultured tissues, diagnosis chips, and biosensors all have something in common: Cells need to be attached to the surface.

Suitable surfaces that induce the adhesion of cells are available, yet, there is no simple method to attach cells onto carriers at defined positions, such as in a particular pattern. The ordering of different cell types in a precise alignment was, until now, extremely complicated. Researchers from the University of Oldenburg have now developed a simple microelectrochemical method by which cells can be "glued" to an exact position on a carrier. Chuan Zhao, Irene Witte, and Gunther Wittstock have also shown that it is possible to adhere, in the same way, a different type of cell at a different location.

The carrier or chip is covered by a continuous thin coating of a material that has ethylene glycol units as free end groups. On such a coated surface, however, it is almost impossible for cells to stick. The Oldenburg research team had found, previously, that treatment with an oxidizing substance such as bromine instantly changes the antistick surface to one that is attractive to cells. This effect can also be applied to small and specific areas if the bromine is directly aimed to come into contact with these defined surface areas. To achieve this, the help of microelectrodes and a solution that contains bromide ions is required. The electrode is positioned close over selected positions of the carrier, and a short potential pulse is applied. As long as the microelectrode is on, the bromide ions will be converted into bromine. The bromine acts on the local area of the surface, however, too little bromine is formed to react extensively with the whole surface. Like a pen, the microelectrode "draws" a pattern on the carrier. If the carrier is incubated with a protein solution, then all the sites that were previously treated by the microelectrode are deposited with the protein from the solution. It is in these positions that the cells then settle. In this way, the researchers were able to cultivate human fibroblasts in a particular pattern. A second fibroblast population could be specifically adhered at further points by repeated electrochemical treatment.

Says Wittstock: "By stepwise site-directed introduction of different cell types onto the surface, our method could facilitate the formation of micropatterned co-cultures and, therefore, contribute to in vitro investigations of multicellular interactions and to tissue engineering".

Gunther Wittstock | alfa
Further information:
http://dx.doi.org/10.1002/anie.200601151

More articles from Life Sciences:

nachricht Ion treatments for cardiac arrhythmia — Non-invasive alternative to catheter-based surgery
20.01.2017 | GSI Helmholtzzentrum für Schwerionenforschung GmbH

nachricht Seeking structure with metagenome sequences
20.01.2017 | DOE/Joint Genome Institute

All articles from Life Sciences >>>

The most recent press releases about innovation >>>

Die letzten 5 Focus-News des innovations-reports im Überblick:

Im Focus: Traffic jam in empty space

New success for Konstanz physicists in studying the quantum vacuum

An important step towards a completely new experimental access to quantum physics has been made at University of Konstanz. The team of scientists headed by...

Im Focus: How gut bacteria can make us ill

HZI researchers decipher infection mechanisms of Yersinia and immune responses of the host

Yersiniae cause severe intestinal infections. Studies using Yersinia pseudotuberculosis as a model organism aim to elucidate the infection mechanisms of these...

Im Focus: Interfacial Superconductivity: Magnetic and superconducting order revealed simultaneously

Researchers from the University of Hamburg in Germany, in collaboration with colleagues from the University of Aarhus in Denmark, have synthesized a new superconducting material by growing a few layers of an antiferromagnetic transition-metal chalcogenide on a bismuth-based topological insulator, both being non-superconducting materials.

While superconductivity and magnetism are generally believed to be mutually exclusive, surprisingly, in this new material, superconducting correlations...

Im Focus: Studying fundamental particles in materials

Laser-driving of semimetals allows creating novel quasiparticle states within condensed matter systems and switching between different states on ultrafast time scales

Studying properties of fundamental particles in condensed matter systems is a promising approach to quantum field theory. Quasiparticles offer the opportunity...

Im Focus: Designing Architecture with Solar Building Envelopes

Among the general public, solar thermal energy is currently associated with dark blue, rectangular collectors on building roofs. Technologies are needed for aesthetically high quality architecture which offer the architect more room for manoeuvre when it comes to low- and plus-energy buildings. With the “ArKol” project, researchers at Fraunhofer ISE together with partners are currently developing two façade collectors for solar thermal energy generation, which permit a high degree of design flexibility: a strip collector for opaque façade sections and a solar thermal blind for transparent sections. The current state of the two developments will be presented at the BAU 2017 trade fair.

As part of the “ArKol – development of architecturally highly integrated façade collectors with heat pipes” project, Fraunhofer ISE together with its partners...

All Focus news of the innovation-report >>>

Anzeige

Anzeige

Event News

Sustainable Water use in Agriculture in Eastern Europe and Central Asia

19.01.2017 | Event News

12V, 48V, high-voltage – trends in E/E automotive architecture

10.01.2017 | Event News

2nd Conference on Non-Textual Information on 10 and 11 May 2017 in Hannover

09.01.2017 | Event News

 
Latest News

Helmholtz International Fellow Award for Sarah Amalia Teichmann

20.01.2017 | Awards Funding

An innovative high-performance material: biofibers made from green lacewing silk

20.01.2017 | Materials Sciences

Ion treatments for cardiac arrhythmia — Non-invasive alternative to catheter-based surgery

20.01.2017 | Life Sciences

VideoLinks
B2B-VideoLinks
More VideoLinks >>>