Forum for Science, Industry and Business

Sponsored by:     3M 
Search our Site:

 

German biotech companies report new results on cultivated liver cells

26.07.2006
Prediction of toxicity and compound responses in hepatocytes continues to be a major concern for the pharmaceutical industry. Non-immortalised primary cells may best represent normal physiology.

The German biotech company Bionas GmbH, Rostock, has made a first study investigating primary human hepatocytes with in vitro test in the Bionas 2500 analyzing system. The human primary hepatocytes were provided by Primacyt GmbH, located in Schwerin, Germany. Primacyt has a unique know-how for serum free long-term human primary hepatocytes cultures.

The Bionas 2500 detects oxygen consumption, acidification and adhesion of cells on six sensor chips in parallel. All parameters are detected continuously and online during long-term measurements. Regeneration and recovery effects are monitored respectively. Primary human hepatocytes were cultured on collagen pre-coated chips in chemically defined Human Hepatocyte Maintenance Medium and, for comparison, in conventional two-dimensional cultures. The sensor chip based in vitro results were compared with standard assays for hepatocytes like albumin release and urea release. The effects of acetaminophen (AAP) were investigated.

The cells were exposed to AAP (50 - 2815 mg/l) for 24 h. Cell respiration was obviously inhibited by AAP concentrations above 500 mg/l whereas cell adhesion was marginally reduced. In conventional two dimensional cultures AAP application reduced albumin release. Comparison of primary human and rat cells shows obviously different hepatotoxic effects of AAP.

It seems that the primary human hepatocytes are less effected as the rat cells or the cell line HepG2. In conclusion the primary human cells are closer at the in-vivo situation as rat cells or artificial cell lines. Distinct differences can be also found if primary rat hepatocytes or the HepG2 cell line are used in the Bionas 2500 analyzing system.

The Bionas 2500 analyzing system clearly predicts compound effects on metabolism of primary cells. The combination of these primary human hepatocytes with the Bionas 2500 analyzing system opened new ways in drug discovery and toxicology.

Elise Kvarnstroem | alfa
Further information:
http://www.scanbalt.org

All articles from Life Sciences >>>

The most recent press releases about innovation >>>

Die letzten 5 Focus-News des innovations-reports im Überblick:

Im Focus: In best circles: First integrated circuit from self-assembled polymer

For the first time, a team of researchers at the Max-Planck Institute (MPI) for Polymer Research in Mainz, Germany, has succeeded in making an integrated circuit (IC) from just a monolayer of a semiconducting polymer via a bottom-up, self-assembly approach.

In the self-assembly process, the semiconducting polymer arranges itself into an ordered monolayer in a transistor. The transistors are binary switches used...

Im Focus: Demonstration of a single molecule piezoelectric effect

Breakthrough provides a new concept of the design of molecular motors, sensors and electricity generators at nanoscale

Researchers from the Institute of Organic Chemistry and Biochemistry of the CAS (IOCB Prague), Institute of Physics of the CAS (IP CAS) and Palacký University...

Im Focus: Hybrid optics bring color imaging using ultrathin metalenses into focus

For photographers and scientists, lenses are lifesavers. They reflect and refract light, making possible the imaging systems that drive discovery through the microscope and preserve history through cameras.

But today's glass-based lenses are bulky and resist miniaturization. Next-generation technologies, such as ultrathin cameras or tiny microscopes, require...

Im Focus: Stem cell divisions in the adult brain seen for the first time

Scientists from the University of Zurich have succeeded for the first time in tracking individual stem cells and their neuronal progeny over months within the intact adult brain. This study sheds light on how new neurons are produced throughout life.

The generation of new nerve cells was once thought to taper off at the end of embryonic development. However, recent research has shown that the adult brain...

Im Focus: Interference as a new method for cooling quantum devices

Theoretical physicists propose to use negative interference to control heat flow in quantum devices. Study published in Physical Review Letters

Quantum computer parts are sensitive and need to be cooled to very low temperatures. Their tiny size makes them particularly susceptible to a temperature...

All Focus news of the innovation-report >>>

Anzeige

Anzeige

VideoLinks
Industry & Economy
Event News

2nd International Conference on High Temperature Shape Memory Alloys (HTSMAs)

15.02.2018 | Event News

Aachen DC Grid Summit 2018

13.02.2018 | Event News

How Global Climate Policy Can Learn from the Energy Transition

12.02.2018 | Event News

 
Latest News

Researchers invent tiny, light-powered wires to modulate brain's electrical signals

21.02.2018 | Life Sciences

The “Holy Grail” of peptide chemistry: Making peptide active agents available orally

21.02.2018 | Life Sciences

Atomic structure of ultrasound material not what anyone expected

21.02.2018 | Materials Sciences

VideoLinks
Science & Research
Overview of more VideoLinks >>>