The German biotech company Bionas GmbH, Rostock, has made a first study investigating primary human hepatocytes with in vitro test in the Bionas® 2500 analyzing system. The human primary hepatocytes were provided by Primacyt GmbH, located in Schwerin, Germany. Primacyt has a unique know-how for serum free long-term human primary hepatocytes cultures.
The Bionas® 2500 detects oxygen consumption, acidification and adhesion of cells on six sensor chips in parallel. All parameters are detected continuously and online during long-term measurements. Regeneration and recovery effects are monitored respectively. Primary human hepatocytes were cultured on collagen pre-coated chips in chemically defined Human Hepatocyte Maintenance Medium and, for comparison, in conventional two-dimensional cultures. The sensor chip based in vitro results were compared with standard assays for hepatocytes like albumin release and urea release. The effects of acetaminophen (AAP) were investigated. The cells were exposed to AAP (50 - 2815 mg/l) for 24 h. Cell respiration was obviously inhibited by AAP concentrations above 500 mg/l whereas cell adhesion was marginally reduced. In conventional two dimensional cultures AAP application reduced albumin release. Comparison of primary human and rat cells shows obviously different hepatotoxic effects of AAP.
It seems that the primary human hepatocytes are less effected as the rat cells or the cell line HepG2. In conclusion the primary human cells are closer at the in-vivo situation as rat cells or artificial cell lines. Distinct differences can be also found if primary rat hepatocytes or the HepG2 cell line are used in the Bionas® 2500 analyzing system.
The Bionas® 2500 analyzing system clearly predicts compound effects on metabolism of primary cells. The combination of these primary human hepatocytes with the Bionas® 2500 analyzing system opened new ways in drug discovery and toxicology.
Bionas was founded in 2001 as spin-off by members of the University of Rostock, Germany. In Rostock we are employing physicists, biochemists, chemists and hard- and software engineers. The Company has more then ten years of experience in the development of semiconductor sensor and its application on living cells. Main investors are Micronas Holding GmbH and GENIUS Venture Capital GmbH.
Bionas develops new tools for applications in the life sciences, drug discovery, cancer research and environment protection providing deeper insights into cell metabolism.
With this world's first ready-to-use sensor chip system, Bionas can reduce the need for animal testing and increase the safety of preclinical studies. So Bionas want to make a significant contribution to environmental protection and fight against cancer.
PRIMACYT Cell Culture Technology GmbH has its roots in HeparCell GmbH, originally founded by 4 private individuals in June 2004. In October 2005 HeparCell GmbH changed its corporate name to PRIMACYT Cell Culture Technology. With HEPAC2 the young biotech start up company has developed a long-term human hepatocyte culture system, that may be used as a biosensor for the analysis of drugs, food additives, and chemicals. HEPAC2 allows the repetitive application of drugs within one cell culture. Thereby, hepatocytes may not only be used for one experiment, but instead can be "recycled" and may be used after a recovery period for a second, a third or even a fourth round of experiments. Thus, the system is designed to reduce the number of animal experiments and to reduce the costs of preclinical studies.
Both companies are member of BioCon Valley, the life science initiative Mecklenburg-Vorpommern, Germany - a partner of ScanBalt fmba, Copenhagen.Contact:
Navigational view of the brain thanks to powerful X-rays
18.10.2017 | Georgia Institute of Technology
Separating methane and CO2 will become more efficient
18.10.2017 | KU Leuven
University of Maryland researchers contribute to historic detection of gravitational waves and light created by event
On August 17, 2017, at 12:41:04 UTC, scientists made the first direct observation of a merger between two neutron stars--the dense, collapsed cores that remain...
Seven new papers describe the first-ever detection of light from a gravitational wave source. The event, caused by two neutron stars colliding and merging together, was dubbed GW170817 because it sent ripples through space-time that reached Earth on 2017 August 17. Around the world, hundreds of excited astronomers mobilized quickly and were able to observe the event using numerous telescopes, providing a wealth of new data.
Previous detections of gravitational waves have all involved the merger of two black holes, a feat that won the 2017 Nobel Prize in Physics earlier this month....
Material defects in end products can quickly result in failures in many areas of industry, and have a massive impact on the safe use of their products. This is why, in the field of quality assurance, intelligent, nondestructive sensor systems play a key role. They allow testing components and parts in a rapid and cost-efficient manner without destroying the actual product or changing its surface. Experts from the Fraunhofer IZFP in Saarbrücken will be presenting two exhibits at the Blechexpo in Stuttgart from 7–10 November 2017 that allow fast, reliable, and automated characterization of materials and detection of defects (Hall 5, Booth 5306).
When quality testing uses time-consuming destructive test methods, it can result in enormous costs due to damaging or destroying the products. And given that...
Using a new cooling technique MPQ scientists succeed at observing collisions in a dense beam of cold and slow dipolar molecules.
How do chemical reactions proceed at extremely low temperatures? The answer requires the investigation of molecular samples that are cold, dense, and slow at...
Scientists from the Max Planck Institute of Quantum Optics, using high precision laser spectroscopy of atomic hydrogen, confirm the surprisingly small value of the proton radius determined from muonic hydrogen.
It was one of the breakthroughs of the year 2010: Laser spectroscopy of muonic hydrogen resulted in a value for the proton charge radius that was significantly...
17.10.2017 | Event News
10.10.2017 | Event News
10.10.2017 | Event News
18.10.2017 | Materials Sciences
18.10.2017 | Physics and Astronomy
18.10.2017 | Physics and Astronomy