The experiment was carried out using mice and produced seven babies, six of which lived to adulthood.
The breakthrough, reported today, Monday July 10, in the academic journal Developmental Cell, helps scientists to understand more about how animals produce sperm. This knowledge has potential applications in the treatment of male infertility.
Karim Nayernia, who has just taken up a post as Professor of Stem Cell Biology at Newcastle University, led the research while in his previous position at Georg-August University in Göttingen, Germany, with Prof. Dr Wolfgang Engel and colleagues from Germany and the UK, including Dr. David Elliott from Newcastle University's Institute of Human Genetics.
Stem cells have the potential to develop into any tissue type in the body and could therefore be used to develop a wide range of medical therapies.
Prof Nayernia, of the Newcastle-Durham-NHS Institute for Stem Cell Biology and Regenerative Medicine*, and his team describe in their paper how they developed a new strategy for generating mature sperm cells in the laboratory using embryonic stem cells from mice. They then went on to test whether this sperm would function in real life.
The team isolated stem cells from a blastocyst, an early-stage embryo that is a cluster of cells only a few days old.
These cells were grown in the laboratory and screened using a special sorting machine. Some had grown into a type of stem cell known as 'spermatogonial stem cells', or early-stage sperm cells.
The spermatogonial cells were singled out, then genetically marked and grown in the laboratory. Some of them grew into cells resembling sperm, known as gametes, which were themselves singled out and highlighted using a genetic marker.
The sperm that had been derived from the embryonic stem cells was then injectd into the female mouse eggs and grown into early-stage embryos.
The early-stage embryos were successfully transplanted into the female mice which produced seven babies. Six developed into adult mice.
The work was funded by the University of Göttingen and the Germany Research Council (DFG).
Prof Nayernia, who originally hails from Shiraz in Southern Iran, said: "This research is particularly important in helping us to understand more about spermatogenesis, the biological process in which sperm is produced. We must know this if we are to get to the root of infertility.
"If we know more about how spermatogonial stem cells turn into sperm cells, this knowledge could be translated into treatments for men who are unable to produce mature sperm, although this is several years down the line. For example, we could isolate a patient's spermatagonial cells using a simple testicular biopsy, encourage them in the laboratory into becoming functional sperm and transplant them back into the patient."
The findings could also inform a field of stem cell research known as nuclear transfer, or therapeutic cloning, which aims to provide tailor-made stem cells to aid disease therapy and infertility. Sperm cells could potentially be created using this method.
Prof Nayernia and his team in Germany were the first in the world to isolate spermatagonial stem cells. The team was also able to show that some of these stem cells, called multipotent adult germline stem cells (maGSCs), turned into heart, muscle, brain and other cells.
Although previous studies have shown that embryonic stem cells grown in the laboratory can become germ cells that give rise to cells resembling sperm cells or gametes, this is the first time scientists have tested whether the gametes really work in real life.
Prof Nayernia added: "Spermatogonial stem cells are extremely promising and more research is needed to establish their full potential."
* The Institute for Stem Cell Biology and Regenerative Medicine (ISCBRM draws together Durham and Newcastle Universities, the Newcastle upon Tyne Hospitals NHS Foundation Trust and other partners in a unique interdisciplinary collaboration to convert stem cell research and technologies into cost-effective, ethically-robust 21st century health solutions to ameliorate degenerative diseases, the effects of ageing and serious injury. The Institute has received substantial funding and other support from One NorthEast.
Novel mechanisms of action discovered for the skin cancer medication Imiquimod
21.10.2016 | Technische Universität München
Second research flight into zero gravity
21.10.2016 | Universität Zürich
Researchers from the Institute for Quantum Computing (IQC) at the University of Waterloo led the development of a new extensible wiring technique capable of controlling superconducting quantum bits, representing a significant step towards to the realization of a scalable quantum computer.
"The quantum socket is a wiring method that uses three-dimensional wires based on spring-loaded pins to address individual qubits," said Jeremy Béjanin, a PhD...
In a paper in Scientific Reports, a research team at Worcester Polytechnic Institute describes a novel light-activated phenomenon that could become the basis for applications as diverse as microscopic robotic grippers and more efficient solar cells.
A research team at Worcester Polytechnic Institute (WPI) has developed a revolutionary, light-activated semiconductor nanocomposite material that can be used...
By forcefully embedding two silicon atoms in a diamond matrix, Sandia researchers have demonstrated for the first time on a single chip all the components needed to create a quantum bridge to link quantum computers together.
"People have already built small quantum computers," says Sandia researcher Ryan Camacho. "Maybe the first useful one won't be a single giant quantum computer...
COMPAMED has become the leading international marketplace for suppliers of medical manufacturing. The trade fair, which takes place every November and is co-located to MEDICA in Dusseldorf, has been steadily growing over the past years and shows that medical technology remains a rapidly growing market.
In 2016, the joint pavilion by the IVAM Microtechnology Network, the Product Market “High-tech for Medical Devices”, will be located in Hall 8a again and will...
'Ferroelectric' materials can switch between different states of electrical polarization in response to an external electric field. This flexibility means they show promise for many applications, for example in electronic devices and computer memory. Current ferroelectric materials are highly valued for their thermal and chemical stability and rapid electro-mechanical responses, but creating a material that is scalable down to the tiny sizes needed for technologies like silicon-based semiconductors (Si-based CMOS) has proven challenging.
Now, Hiroshi Funakubo and co-workers at the Tokyo Institute of Technology, in collaboration with researchers across Japan, have conducted experiments to...
14.10.2016 | Event News
14.10.2016 | Event News
12.10.2016 | Event News
21.10.2016 | Health and Medicine
21.10.2016 | Information Technology
21.10.2016 | Materials Sciences