Despite its importance in everyday human function, very little is known about the regulation of sleep. In search of the underlying brain region responsible for sleep regulation, senior author Amita Sehgal, PhD, Professor of Neuroscience and a Howard Hughes Medical Institute (HHMI) Investigator, and colleagues turned their attention to the fruit fly.
"Fruit flies and humans share similar resting patterns," explains Sehgal. "Like humans, the sleeping states of fruit flies are characterized by periods of immobility over a twenty-four hour period, during which the fruit flies demonstrate reduced responsiveness to sensory stimuli."
By tinkering with the gene expression of multiple regions of the fruit fly brain, the research team was able to zero in on the adult mushroom body as the sleep center of the brain. They reported their findings in last week's issue of Nature.
To locate the brain region involved in sleep regulation, Sehgal manipulated the activity of an enzyme known as protein kinase A (PKA). Previous work in Sehgal's lab revealed that the higher the level of PKA activity, the lower the period of immobility, or sleep, in the fruit fly. By building upon this work, Sehgal and others set out to increase PKA activity in various regions of the brain and examine the subsequent sleeping patterns in the fruit flies. "Sleeping fruit flies" were defined as those that remained immobile for at least five minutes.
"From the beginning, we took the unbiased approach," explains Sehgal. "We targeted PKA activity to different areas of the fly brain to find out where PKA acts to regulate sleep."
Sehgal was able to selectively turn on PKA activity in a variety of brain locations, which promoted PKA expression in designated regions. Of the different regions targeted, only two regions, both present in the adult mushroom bodies, led to changes in sleeping patterns of fruit flies. The fly mushroom body has been likened to the human hippocampus. The changes in sleep caused by the increased PKA activity in the adult mushroom bodies highlighted this region as the sleep-regulating region of the fruit fly brain.
When PKA activity was expressed in one of the two distinct regions of the mushroom bodies, increased sleep occurred while expression in the other region decreased sleep in the flies. Thus, the adult mushroom bodies possess both sleep-promoting and sleep-inhibiting areas.
"Although people typically think of mushroom bodies as possessing similar functions to the human hippocampus, the site where long-term memories are made, our lab tends to think of the mushroom bodies functioning more like the thalamus, the relay station through which most sensory input to the brain is targeted," explains Sehgal. "Previous research links the thalamus to a role in human sleep." (There is no human structure that is anatomically similar to the adult mushroom bodies of fruit flies.)
Identifying the role of adult mushroom bodies in sleep may offer insight into how and why sleep is needed to assist in learning and memory consolidation. In mammals, sleep deprivation suppresses the performance of learned tasks, and sleep permits memory consolidation. Distinct anatomical regions of adult mushroom bodies have been shown to be important for at least some forms of memory in fruit flies.
In a paper also published last week in Current Biology, Sehgal and colleagues showed that serotonin affects sleep in fruit flies by acting at the site of the adult mushroom bodies.
Sehgal's lab reduced the function of three types of serotonin receptors in the brains of fruit flies (5HT1A, 5HT1B, and 5HT2). The reduced 5HT1A receptor activity in the fruit flies led to fragmented and reduced overall sleep. In essence, the fruit flies tossed and turned in their sleep. But, the flies with reduced 5HT1B and 5HT2 receptor activity displayed no change in their sleeping pattern. Penn researchers were able to treat the fruit flies to a good night's sleep by administering serotonin to the adult mushroom bodies.
The finding that serotonin plays a role in increasing sleep in fruit flies offers hope for the future of therapeutics for sleep disorders. "Serotonin may also promote sleep in humans," suggests Sehgal. "This may explain why serotonin-increasing antidepressants increase sleep."
Future work by Sehgal's lab will attempt to look for a connection among sleep, serotonin, and learning, and memory, while looking deeper into the cellular and molecular activity that enables mushroom bodies to regulate sleep.
Karen Kreeger | EurekAlert!
Single-stranded DNA and RNA origami go live
15.12.2017 | Wyss Institute for Biologically Inspired Engineering at Harvard
New antbird species discovered in Peru by LSU ornithologists
15.12.2017 | Louisiana State University
DNA molecules that follow specific instructions could offer more precise molecular control of synthetic chemical systems, a discovery that opens the door for engineers to create molecular machines with new and complex behaviors.
Researchers have created chemical amplifiers and a chemical oscillator using a systematic method that has the potential to embed sophisticated circuit...
MPQ scientists achieve long storage times for photonic quantum bits which break the lower bound for direct teleportation in a global quantum network.
Concerning the development of quantum memories for the realization of global quantum networks, scientists of the Quantum Dynamics Division led by Professor...
Researchers have developed a water cloaking concept based on electromagnetic forces that could eliminate an object's wake, greatly reducing its drag while...
Tiny pores at a cell's entryway act as miniature bouncers, letting in some electrically charged atoms--ions--but blocking others. Operating as exquisitely sensitive filters, these "ion channels" play a critical role in biological functions such as muscle contraction and the firing of brain cells.
To rapidly transport the right ions through the cell membrane, the tiny channels rely on a complex interplay between the ions and surrounding molecules,...
The miniaturization of the current technology of storage media is hindered by fundamental limits of quantum mechanics. A new approach consists in using so-called spin-crossover molecules as the smallest possible storage unit. Similar to normal hard drives, these special molecules can save information via their magnetic state. A research team from Kiel University has now managed to successfully place a new class of spin-crossover molecules onto a surface and to improve the molecule’s storage capacity. The storage density of conventional hard drives could therefore theoretically be increased by more than one hundred fold. The study has been published in the scientific journal Nano Letters.
Over the past few years, the building blocks of storage media have gotten ever smaller. But further miniaturization of the current technology is hindered by...
11.12.2017 | Event News
08.12.2017 | Event News
07.12.2017 | Event News
15.12.2017 | Power and Electrical Engineering
15.12.2017 | Materials Sciences
15.12.2017 | Life Sciences