Forum for Science, Industry and Business

Sponsored by:     3M 
Search our Site:

 

Researchers report technique for freezing and preserving genetically enhanced pig embryos

05.05.2006


Researchers led by a University of Missouri-Columbia professor of reproductive biotechnology have reported success in freezing and preserving swine embryos that were created by in vitro techniques and that carried modified genetic material. After thawing and transfer to a surrogate mother, some of the embryos went on to produce live piglets with new genetic traits.



In a paper posted today (May 3, 2006) on the Web site of Biology of Reproduction--Papers in Press, a team headed by Dr. Randall Prather, co-director of the National Swine Resource and Research Center, note that their technique could prove valuable in allowing genetically enhanced swine to be transported as embryos across the country and throughout the world.

Because many embryos are needed to produce a successful pregnancy in surrogate female pigs, this new procedure will enable workers to collect an adequate number of altered embryos and store them until they can be transplanted to a surrogate.


Swine embryos have been difficult to freeze and preserve because of their sensitivity to cold due to a high level of lipids in the cells of the embryos. It was expected that it would be even more difficult to freeze and preserve swine embryos that had been produced by in vitro methods.

Dr. Prather’s team overcame the obstacles to freezing swine embryos by first removing lipids from unfertilized eggs before fertilizing them with muscle cells from a male pig containing modified genetic material. The resulting embryos were then frozen at the blastocyst stage.

Upon thawing, embryos were transferred to surrogate female swine. Embryos placed in the oviducts of two surrogates led to pregnancies that produced two piglets in one litter and eight piglets in another.

Testing confirmed that the piglets carried the modified genetic material and that the new gene had altered the fatty-acid content in tissues from the piglets.

With ten piglets from 163 frozen and preserved embryos, the researchers feel that their procedure appears to work well, although further studies will be needed to refine the technique.

Dr. Prather and his team expect that freezing and preserving swine embryos will enable wide dissemination of swine with genetic traits that are commercially valuable and also important for biomedical research involving this increasingly relevant animal model for human disorders.

Jennifer Faddis | EurekAlert!
Further information:
http://www.ssr.org/

More articles from Life Sciences:

nachricht Unique genome architectures after fertilisation in single-cell embryos
30.03.2017 | IMBA - Institut für Molekulare Biotechnologie der Österreichischen Akademie der Wissenschaften GmbH

nachricht Transport of molecular motors into cilia
28.03.2017 | Aarhus University

All articles from Life Sciences >>>

The most recent press releases about innovation >>>

Die letzten 5 Focus-News des innovations-reports im Überblick:

Im Focus: A Challenging European Research Project to Develop New Tiny Microscopes

The Institute of Semiconductor Technology and the Institute of Physical and Theoretical Chemistry, both members of the Laboratory for Emerging Nanometrology (LENA), at Technische Universität Braunschweig are partners in a new European research project entitled ChipScope, which aims to develop a completely new and extremely small optical microscope capable of observing the interior of living cells in real time. A consortium of 7 partners from 5 countries will tackle this issue with very ambitious objectives during a four-year research program.

To demonstrate the usefulness of this new scientific tool, at the end of the project the developed chip-sized microscope will be used to observe in real-time...

Im Focus: Giant Magnetic Fields in the Universe

Astronomers from Bonn and Tautenburg in Thuringia (Germany) used the 100-m radio telescope at Effelsberg to observe several galaxy clusters. At the edges of these large accumulations of dark matter, stellar systems (galaxies), hot gas, and charged particles, they found magnetic fields that are exceptionally ordered over distances of many million light years. This makes them the most extended magnetic fields in the universe known so far.

The results will be published on March 22 in the journal „Astronomy & Astrophysics“.

Galaxy clusters are the largest gravitationally bound structures in the universe. With a typical extent of about 10 million light years, i.e. 100 times the...

Im Focus: Tracing down linear ubiquitination

Researchers at the Goethe University Frankfurt, together with partners from the University of Tübingen in Germany and Queen Mary University as well as Francis Crick Institute from London (UK) have developed a novel technology to decipher the secret ubiquitin code.

Ubiquitin is a small protein that can be linked to other cellular proteins, thereby controlling and modulating their functions. The attachment occurs in many...

Im Focus: Perovskite edges can be tuned for optoelectronic performance

Layered 2D material improves efficiency for solar cells and LEDs

In the eternal search for next generation high-efficiency solar cells and LEDs, scientists at Los Alamos National Laboratory and their partners are creating...

Im Focus: Polymer-coated silicon nanosheets as alternative to graphene: A perfect team for nanoelectronics

Silicon nanosheets are thin, two-dimensional layers with exceptional optoelectronic properties very similar to those of graphene. Albeit, the nanosheets are less stable. Now researchers at the Technical University of Munich (TUM) have, for the first time ever, produced a composite material combining silicon nanosheets and a polymer that is both UV-resistant and easy to process. This brings the scientists a significant step closer to industrial applications like flexible displays and photosensors.

Silicon nanosheets are thin, two-dimensional layers with exceptional optoelectronic properties very similar to those of graphene. Albeit, the nanosheets are...

All Focus news of the innovation-report >>>

Anzeige

Anzeige

Event News

International Land Use Symposium ILUS 2017: Call for Abstracts and Registration open

20.03.2017 | Event News

CONNECT 2017: International congress on connective tissue

14.03.2017 | Event News

ICTM Conference: Turbine Construction between Big Data and Additive Manufacturing

07.03.2017 | Event News

 
Latest News

'On-off switch' brings researchers a step closer to potential HIV vaccine

30.03.2017 | Health and Medicine

Penn studies find promise for innovations in liquid biopsies

30.03.2017 | Health and Medicine

An LED-based device for imaging radiation induced skin damage

30.03.2017 | Medical Engineering

VideoLinks
B2B-VideoLinks
More VideoLinks >>>