LGC launches generic DNA-based proficiency testing scheme for quantitative PCR

Developed by LGC as part of the DTI-funded Measurements for Biotechnology (MfB) programme 2004-2007, this innovative approach is the first DNA-based PT scheme able to provide test materials applicable to a significantly wide range of sectors, beneficial to analysts working in clinical, pharmaceutical, forensic, academic and public health laboratories. LGC has established a reputation for setting up and running successful PT initiatives and, as the UK’s National Measurement Institute for chemical and biochemical analysis, promotes international standards for analytical measurement for use in trade, industry, academia and government.

The new scheme encompasses DNA extraction from a proprietary generic matrix and Q-PCR quantification of extracted samples. Participants will be provided with synthetic analytes and matrices, together with target-specific reagents for real-time PCR quantification. The scheme will support a range of detection chemistries and platforms and will operate in accordance with the principles of ILAC Guide 13 and ISO Guide 43-1.

PT schemes enable laboratories to monitor and improve analytical measurements by providing an external quality assessment system to complement their own internal quality control processes. Participation in a PT scheme is integral to laboratory accreditation and often supports regulatory requirements, such as the UK Environment Agency’s Monitoring Certification Scheme (MCERTS).

On announcing the new scheme, Dr John Marriott, Director of Analytical Technology at LGC, and Government Chemist, said: “As the first cross-sectoral DNA-based PT scheme it is a valuable aid to good quality measurements in the technically difficult field of quantitative DNA analysis. Participation in the scheme will help analysts to pinpoint any area of weakness in their procedures and set-up.”