Forum for Science, Industry and Business

Sponsored by:     3M 
Search our Site:

 

Prediction of a prokaryotic RNA-silencing system

16.03.2006


Researchers have used computational methods to predict what could be a prokaryotic RNA-silencing mechanism similar to the eukaryotic RNA- interference system. A study published today in the open access journal with a novel system of peer review, Biology Direct, provides the first strong evidence that a type of tandem repeats found in archaea and bacteria, the Clustered Regularly Interspaced Short Palindrome Repeats (CRISPR), might act in conjunction with the CRISPR-associated (cas) family of genes as a defence mechanism against phage and plasmid RNA. A number of Cas proteins are shown to contain domains that suggest a functional similarity to eukaryotic proteins involved in the eukaryotic RNA-interference system.



Kira Makarova and other members of a group led by Eugene Koonin, from the National Institutes of Health, Bethesda, USA, carried out a comparative genomic analysis of CRISPR and cas genes in archaeal and bacterial genome sequences retrieved from National Center for Biotechnology Information (NCBI) databases.

Makarova et al. identified a number of cas genes that are always located close to CRISPR clusters and encode proteins potentially involved in RNA-processing mechanisms such as unwinding and cleaving. These proteins might be functionally similar to eukaryotic enzymes involved in the RNA-interference system – Makarova et al. identify an analog to the eukaryotic RNAi protein Dicer and several potential analogs to the eukaryotic RNAi protein Slicer. But they are not homologous to Dicer and Slicer as they have no sequence similarity with them.


It has been shown that a proportion of inserts in CRISPR units are similar to fragments of viral or plasmid genomes. Makarova et al. extend these observations and propose that all CRISPR inserts are derived from viruses or plasmids but this is not immediately obvious because most of these agents are still unknown. They speculate that the inserts are transcribed and silence phage or plasmid sequences via the formation of a duplex, which is then cleaved by Cas proteins to destroy the foreign RNA.

Juliette Savin | EurekAlert!
Further information:
http://www.biomedcentral.com

More articles from Life Sciences:

nachricht At last, butterflies get a bigger, better evolutionary tree
16.02.2018 | Florida Museum of Natural History

nachricht New treatment strategies for chronic kidney disease from the animal kingdom
16.02.2018 | Veterinärmedizinische Universität Wien

All articles from Life Sciences >>>

The most recent press releases about innovation >>>

Die letzten 5 Focus-News des innovations-reports im Überblick:

Im Focus: Demonstration of a single molecule piezoelectric effect

Breakthrough provides a new concept of the design of molecular motors, sensors and electricity generators at nanoscale

Researchers from the Institute of Organic Chemistry and Biochemistry of the CAS (IOCB Prague), Institute of Physics of the CAS (IP CAS) and Palacký University...

Im Focus: Hybrid optics bring color imaging using ultrathin metalenses into focus

For photographers and scientists, lenses are lifesavers. They reflect and refract light, making possible the imaging systems that drive discovery through the microscope and preserve history through cameras.

But today's glass-based lenses are bulky and resist miniaturization. Next-generation technologies, such as ultrathin cameras or tiny microscopes, require...

Im Focus: Stem cell divisions in the adult brain seen for the first time

Scientists from the University of Zurich have succeeded for the first time in tracking individual stem cells and their neuronal progeny over months within the intact adult brain. This study sheds light on how new neurons are produced throughout life.

The generation of new nerve cells was once thought to taper off at the end of embryonic development. However, recent research has shown that the adult brain...

Im Focus: Interference as a new method for cooling quantum devices

Theoretical physicists propose to use negative interference to control heat flow in quantum devices. Study published in Physical Review Letters

Quantum computer parts are sensitive and need to be cooled to very low temperatures. Their tiny size makes them particularly susceptible to a temperature...

Im Focus: Autonomous 3D scanner supports individual manufacturing processes

Let’s say the armrest is broken in your vintage car. As things stand, you would need a lot of luck and persistence to find the right spare part. But in the world of Industrie 4.0 and production with batch sizes of one, you can simply scan the armrest and print it out. This is made possible by the first ever 3D scanner capable of working autonomously and in real time. The autonomous scanning system will be on display at the Hannover Messe Preview on February 6 and at the Hannover Messe proper from April 23 to 27, 2018 (Hall 6, Booth A30).

Part of the charm of vintage cars is that they stopped making them long ago, so it is special when you do see one out on the roads. If something breaks or...

All Focus news of the innovation-report >>>

Anzeige

Anzeige

VideoLinks
Industry & Economy
Event News

2nd International Conference on High Temperature Shape Memory Alloys (HTSMAs)

15.02.2018 | Event News

Aachen DC Grid Summit 2018

13.02.2018 | Event News

How Global Climate Policy Can Learn from the Energy Transition

12.02.2018 | Event News

 
Latest News

Fingerprints of quantum entanglement

16.02.2018 | Information Technology

'Living bandages': NUST MISIS scientists develop biocompatible anti-burn nanofibers

16.02.2018 | Health and Medicine

Hubble sees Neptune's mysterious shrinking storm

16.02.2018 | Physics and Astronomy

VideoLinks
Science & Research
Overview of more VideoLinks >>>