Manchester scientists create new bio-gel for 3D cell culture
Scientists at The University of Manchester have created a new type of ‘bio-gel’ which provides a pH neutral environment for culturing cells in 3D, as published in the journal Advanced Materials (March 2006).
The gel is the first pH neutral material made from combinations of dipeptides (pairs of amino acids) to provide an environment in which cells can be cultured under physiological conditions.
Uniquely, the gel mimics the properties of cell scaffolds which naturally occur in the body and has potential applications for wound healing and tissue engineering.
Cell scaffolds, known as the extra cellular matrix (ECM), are naturally produced by the body to grow new cells in order to repair damaged tissue. Like the ECM, the gel acts like a scaffold in which cells can grow.
In their paper, ‘Nanostructured Hydrogels for Three-Dimensional Cell Culture Through Self-Assembly of Fluorenylmethoxycarbonyl-Dipeptides’, Dr Rein Ulijn and collaborators describe how the gel is created through a process of self-assembly.
Dr Ulijn said: “We have used combinations of modified dipeptides which act like building blocks and spontaneously assemble into nanometer sized fibres when exposed to physiological conditions, to create a fibrous gel-like structure in which cells can be cultured. Because thismaterial is made up of 99% water and is pH neutral, it is compatible with biological systems.
“By using dipeptide building blocks instead of much larger oligo-peptides used by other researchers, we have greater control over the fibrous architecture and the physical properties of the gels. These materials offer us great potential for future applications in wound healing and regenerative medicine.”
Dr Ulijn and his collaborators have successfully cultured cartilage cells using the gel. They found that both the properties of the gels formed and the cell response to the gels could be controlled by using different combinations of di-peptides. The team recently received a £630k award from EPSRC to develop the gels further.
Simon Hunter | alfa
The most recent press releases about innovation >>>
Die letzten 5 Focus-News des innovations-reports im Überblick:
Whether you call it effervescent, fizzy, or sparkling, carbonated water is making a comeback as a beverage. Aside from quenching thirst, researchers at the University of Illinois at Urbana-Champaign have discovered a new use for these "bubbly" concoctions that will have major impact on the manufacturer of the world's thinnest, flattest, and one most useful materials -- graphene.
As graphene's popularity grows as an advanced "wonder" material, the speed and quality at which it can be manufactured will be paramount. With that in mind,...
Physicists at the University of Bonn have managed to create optical hollows and more complex patterns into which the light of a Bose-Einstein condensate flows. The creation of such highly low-loss structures for light is a prerequisite for complex light circuits, such as for quantum information processing for a new generation of computers. The researchers are now presenting their results in the journal Nature Photonics.
Light particles (photons) occur as tiny, indivisible portions. Many thousands of these light portions can be merged to form a single super-photon if they are...
For the first time, scientists have shown that circular RNA is linked to brain function. When a RNA molecule called Cdr1as was deleted from the genome of mice, the animals had problems filtering out unnecessary information – like patients suffering from neuropsychiatric disorders.
While hundreds of circular RNAs (circRNAs) are abundant in mammalian brains, one big question has remained unanswered: What are they actually good for? In the...
An experimental small satellite has successfully collected and delivered data on a key measurement for predicting changes in Earth's climate.
The Radiometer Assessment using Vertically Aligned Nanotubes (RAVAN) CubeSat was launched into low-Earth orbit on Nov. 11, 2016, in order to test new...
A study led by scientists of the Max Planck Institute for the Structure and Dynamics of Matter (MPSD) at the Center for Free-Electron Laser Science in Hamburg presents evidence of the coexistence of superconductivity and “charge-density-waves” in compounds of the poorly-studied family of bismuthates. This observation opens up new perspectives for a deeper understanding of the phenomenon of high-temperature superconductivity, a topic which is at the core of condensed matter research since more than 30 years. The paper by Nicoletti et al has been published in the PNAS.
Since the beginning of the 20th century, superconductivity had been observed in some metals at temperatures only a few degrees above the absolute zero (minus...