Forum for Science, Industry and Business

Sponsored by:     3M 
Search our Site:

 

Stem cells from muscles can repair cartilage

30.01.2006


Study finds genetically engineered muscle-derived stem cells improved cartilage repair in rats



Damage to articular cartilage (cartilage covering the ends of bones where they meet in a joint) frequently occurs due to injury or illness, and can lead to degenerative disease. Treatments and experimental approaches to repair this articular cartilage have achieved limited results, but currently there is no method to fully restore this type of injured cartilage. Tissue engineering involving the delivery of therapeutic proteins to the injured site is a promising new approach to repairing articular cartilage. Previous studies have suggested that muscles contain stem cells that can develop in various ways, including into cells that lead to the formation of bone. In a study published in the February 2006 issue of Arthritis & Rheumatism (http://www.interscience.wiley.com/journal/arthritis), researchers designed a study using muscle-derived stem cells (MDSCs) genetically engineered with a therapeutic protein in an effort to repair articular cartilage defects in rats.

Led by Johnny Huard, PhD, director of the Growth and Development Laboratory at Children’s Hospital of Pittsburgh and an associate professor in the departments of Orthopaedic Surgery and Molecular Genetics and Biochemistry and Bioengineering at the University of Pittsburgh School of Medicine, researchers induced damage to the knee joints in 36 12-week-old rats and divided them into three groups. Group 1 was treated with MDSCs embedded in fibrin glue. Group 2 was treated with MDSCs that had been cultured from 3-week-old rats and genetically engineered to express bone morphogenetic protein-4 (BMP-4). Group 3, the control group, was treated with fibrin glue.


The results showed well-integrated repaired tissue in the MDSC-B4 treatment group 8, 12 and 24 weeks after surgery, while the other two groups did not show as much improvement initially and by 24 weeks had deteriorated. "This finding indicates that continuous endogenous BMP-4 supplied by MDSCs genetically modified to express BMP-4 over an extended period of time can enhance articular cartilage healing," the authors state. They note that using fibrin glue enabled the cells to settle even in narrow clefts and has no adverse effects on cell viability.

The study also included culturing MDSC and MDSC-B4 in vitro in three different types of media and found that MDSC-B4 were able to differentiate into chondrogenic cells (cells that develop into cartilage) depending on the type of medium in which they grew. The addition of transforming grown factor â1 (TGFâ1) to MDSC-B4 did not enhance chondrogenic differentiation.

The authors conclude that skeletal muscle is a promising source of cells that can differentiate into cartilage-producing cells. "The MDSCs used here served as good carriers of a therapeutic gene and enabled the delivery of appropriate amounts of BMP-4 protein to the injury site," they state, adding that the improved repair lasted for 24 weeks following transplantation. Further studies conducted by the authors (not yet published) have produced similar results. They conclude: "These findings suggest that BMP-4 gene therapy based on retrovirally transduced MDSCs is a potential strategy by which to improve articular cartilage healing."

In an accompanying editorial in the same issue, Mary B. Goldring of the New England Baptist Bone and Joint Institute and Harvard Medical School in Boston, MA, notes that although stem cells derived from bone marrow have been extensively investigated for the repair of cartilage defects, the current study is the first to investigate using enriched stem cells from muscle. The author states that the results are encouraging, especially the successful use of fibrin glue to fill in the defect, and that the study "provides proof-of-principle for performing MDSC implantation in cartilage of adult humans, since 12-week-old rats are considered to be young adults." Although recent studies have suggested that fatty tissue and tissue lining the space between joints (synovium) might also be sources of stem cells, obtaining these cells is more invasive than muscle biopsies. The author also notes that patients could potentially serve as donors to themselves, since the current study used MDSCs from juvenile rats and other studies have shown such transplants to be problematic in adults. She concludes: "Thus, further work is warranted to determine the chondroprogenitor potential of MDSCs in adult humans and their capacity to form cartilage in vivo."

Amy Molnar | EurekAlert!
Further information:
http://www.wiley.com
http://www.interscience.wiley.com/journal/arthritis

More articles from Life Sciences:

nachricht Symbiotic bacteria: from hitchhiker to beetle bodyguard
28.04.2017 | Johannes Gutenberg-Universität Mainz

nachricht Nose2Brain – Better Therapy for Multiple Sclerosis
28.04.2017 | Fraunhofer-Institut für Grenzflächen- und Bioverfahrenstechnik IGB

All articles from Life Sciences >>>

The most recent press releases about innovation >>>

Die letzten 5 Focus-News des innovations-reports im Überblick:

Im Focus: Making lightweight construction suitable for series production

More and more automobile companies are focusing on body parts made of carbon fiber reinforced plastics (CFRP). However, manufacturing and repair costs must be further reduced in order to make CFRP more economical in use. Together with the Volkswagen AG and five other partners in the project HolQueSt 3D, the Laser Zentrum Hannover e.V. (LZH) has developed laser processes for the automatic trimming, drilling and repair of three-dimensional components.

Automated manufacturing processes are the basis for ultimately establishing the series production of CFRP components. In the project HolQueSt 3D, the LZH has...

Im Focus: Wonder material? Novel nanotube structure strengthens thin films for flexible electronics

Reflecting the structure of composites found in nature and the ancient world, researchers at the University of Illinois at Urbana-Champaign have synthesized thin carbon nanotube (CNT) textiles that exhibit both high electrical conductivity and a level of toughness that is about fifty times higher than copper films, currently used in electronics.

"The structural robustness of thin metal films has significant importance for the reliable operation of smart skin and flexible electronics including...

Im Focus: Deep inside Galaxy M87

The nearby, giant radio galaxy M87 hosts a supermassive black hole (BH) and is well-known for its bright jet dominating the spectrum over ten orders of magnitude in frequency. Due to its proximity, jet prominence, and the large black hole mass, M87 is the best laboratory for investigating the formation, acceleration, and collimation of relativistic jets. A research team led by Silke Britzen from the Max Planck Institute for Radio Astronomy in Bonn, Germany, has found strong indication for turbulent processes connecting the accretion disk and the jet of that galaxy providing insights into the longstanding problem of the origin of astrophysical jets.

Supermassive black holes form some of the most enigmatic phenomena in astrophysics. Their enormous energy output is supposed to be generated by the...

Im Focus: A Quantum Low Pass for Photons

Physicists in Garching observe novel quantum effect that limits the number of emitted photons.

The probability to find a certain number of photons inside a laser pulse usually corresponds to a classical distribution of independent events, the so-called...

Im Focus: Microprocessors based on a layer of just three atoms

Microprocessors based on atomically thin materials hold the promise of the evolution of traditional processors as well as new applications in the field of flexible electronics. Now, a TU Wien research team led by Thomas Müller has made a breakthrough in this field as part of an ongoing research project.

Two-dimensional materials, or 2D materials for short, are extremely versatile, although – or often more precisely because – they are made up of just one or a...

All Focus news of the innovation-report >>>

Anzeige

Anzeige

Event News

Fighting drug resistant tuberculosis – InfectoGnostics meets MYCO-NET² partners in Peru

28.04.2017 | Event News

Expert meeting “Health Business Connect” will connect international medical technology companies

20.04.2017 | Event News

Wenn der Computer das Gehirn austrickst

18.04.2017 | Event News

 
Latest News

Wireless power can drive tiny electronic devices in the GI tract

28.04.2017 | Medical Engineering

Ice cave in Transylvania yields window into region's past

28.04.2017 | Earth Sciences

Nose2Brain – Better Therapy for Multiple Sclerosis

28.04.2017 | Life Sciences

VideoLinks
B2B-VideoLinks
More VideoLinks >>>