Forum for Science, Industry and Business

Sponsored by:     3M 
Search our Site:

 

New model of prostate cancer helps identify promising pain treatment

02.12.2005


Researchers have developed a new line of prostate cancer cells that they hope will provide a better model to study the disease.



This new cancer-cell line has already provided some help. One new study in mice identified a promising possible therapy to reduce skeletal pain that accompanies prostate cancer. Scientists found that a substance called anti-nerve growth factor appeared to be more effective in controlling pain in mice than even morphine.

But the work would not have been possible without the new cell line, said Tom Rosol , a study co-author and a professor of veterinary medicine at Ohio State University.


Armed with this new cell line, scientists will be able to more directly study how prostate cancer affects the body, said Rosol, whose laboratory developed the cell line.

Metastatic bone tumors are a common manifestation in patients with late-stage breast cancer or prostate cancer. “Metastasis” means that cancer has spread from its original site to other areas of the body. But breast cancer typically destroys bone at tumor sites, whereas prostate cancer tumors that spread to bone induce abnormal bone growth.

Currently, most models used to study prostate cancer do not mimic the human condition and the resulting bone metastases. Most of these models really mimic the spread of breast cancer since the bone metastases in that disease are associated with bone loss rather than bone growth.

“Even though there is more bone at the sites of prostate cancer tumors, this formation still damages the bone,” said Rosol, who is also dean of the College of Veterinary Medicine at Ohio State . “The new growth compresses nerves, making it terribly painful for the patient.”

The results appear in a recent issue of the journal Cancer Research. The study was led by Patrick Mantyh, a professor of preventive sciences at the University of Minnesota.

Only two mammals are known to develop prostate cancer –- men and dogs. Rosol’s laboratory created a cell line from prostate tumors that had developed in a dog’s bones. The researchers call this line of cells ACE-1.

In the current study, researchers at the University of Minnesota injected the ACE-1 cells directly into the femurs, or thigh bones, of male mice. These mice were specially bred to lack an immune system, leaving them vulnerable to developing prostate cancer. While the femur is the biggest bone in the body -- and therefore the easiest to study in this case -- prostate cancer can affect any bone in the body.

It took about a week for the prostate tumors to develop in the mice. At that point, the researchers began treating mice with anti-nerve growth factor (NGF). Anti-NGF is a molecule that naturally occurs in the body, where it promotes the survival and growth of nerves. An additional group of mice was treated with morphine. Control mice, which also had prostate cancer, were given a sterile saline solution instead of either anti-NGF or morphine.

The researchers wanted to know what kind of effect, if any, anti-NGF had on pain-related behaviors, tumor growth, bone formation and bone destruction in the mice.

The researchers watched mice at different points in the study to see if they showed any kind of pain-related behavior. The researchers kept track of how much time each mouse spent favoring its affected leg – how often the mouse lifted its leg while standing still, and for how long it held this leg aloft.

Mice treated with anti-NGF spent less time favoring their affected leg than did mice that were given morphine. In some cases, the time that a mouse treated with anti-NGF spent favoring its affected leg was half that of a mouse treated with morphine.

This suggests that anti-NGF therapy may be effective in reducing pain, thereby helping to enhance the quality of life in patients with bone pain caused by prostate cancer.

All of the animals were euthanized about two weeks after receiving the first round of ACE-1 injections. At that point, the researchers removed the affected thigh bone from each mouse in order to analyze the bone’s density. Bone density corresponded with the number of tumors in the bone – the denser the bone, the more tumors it had.

Results from the density analysis showed that anti-NGF therapy did not stop prostate cancer from progressing, nor did it decrease bone formation caused by the disease.

Why prostate cancer causes excess bone to form remains a mystery, but having the ACE-1 model may help researchers learn why it happens.

“Bone is often the only clinically detectable site of the spread of prostate cancer,” Rosol said. “Understanding why this happens is not only important for cancer patients, but also for scientists who are trying to understand how bone responds to different biochemical factors.”

Rosol conducted the study with researchers from the University of Minnesota; the Veterans Affairs Medical Center in Minneapolis; and with Rinat Neuroscience Corporation, in Palo Alto, Calif.

This work was supported by a grant from the National Institutes of Health, the MinCREST program at the University of Minnesota and a Merit Review from the Veterans Administration.

Thomas Rosol | EurekAlert!
Further information:
http://www.ohio-state.edu

More articles from Life Sciences:

nachricht Rainbow colors reveal cell history: Uncovering β-cell heterogeneity
22.09.2017 | DFG-Forschungszentrum für Regenerative Therapien TU Dresden

nachricht The pyrenoid is a carbon-fixing liquid droplet
22.09.2017 | Max-Planck-Institut für Biochemie

All articles from Life Sciences >>>

The most recent press releases about innovation >>>

Die letzten 5 Focus-News des innovations-reports im Überblick:

Im Focus: The pyrenoid is a carbon-fixing liquid droplet

Plants and algae use the enzyme Rubisco to fix carbon dioxide, removing it from the atmosphere and converting it into biomass. Algae have figured out a way to increase the efficiency of carbon fixation. They gather most of their Rubisco into a ball-shaped microcompartment called the pyrenoid, which they flood with a high local concentration of carbon dioxide. A team of scientists at Princeton University, the Carnegie Institution for Science, Stanford University and the Max Plank Institute of Biochemistry have unravelled the mysteries of how the pyrenoid is assembled. These insights can help to engineer crops that remove more carbon dioxide from the atmosphere while producing more food.

A warming planet

Im Focus: Highly precise wiring in the Cerebral Cortex

Our brains house extremely complex neuronal circuits, whose detailed structures are still largely unknown. This is especially true for the so-called cerebral cortex of mammals, where among other things vision, thoughts or spatial orientation are being computed. Here the rules by which nerve cells are connected to each other are only partly understood. A team of scientists around Moritz Helmstaedter at the Frankfiurt Max Planck Institute for Brain Research and Helene Schmidt (Humboldt University in Berlin) have now discovered a surprisingly precise nerve cell connectivity pattern in the part of the cerebral cortex that is responsible for orienting the individual animal or human in space.

The researchers report online in Nature (Schmidt et al., 2017. Axonal synapse sorting in medial entorhinal cortex, DOI: 10.1038/nature24005) that synapses in...

Im Focus: Tiny lasers from a gallery of whispers

New technique promises tunable laser devices

Whispering gallery mode (WGM) resonators are used to make tiny micro-lasers, sensors, switches, routers and other devices. These tiny structures rely on a...

Im Focus: Ultrafast snapshots of relaxing electrons in solids

Using ultrafast flashes of laser and x-ray radiation, scientists at the Max Planck Institute of Quantum Optics (Garching, Germany) took snapshots of the briefest electron motion inside a solid material to date. The electron motion lasted only 750 billionths of the billionth of a second before it fainted, setting a new record of human capability to capture ultrafast processes inside solids!

When x-rays shine onto solid materials or large molecules, an electron is pushed away from its original place near the nucleus of the atom, leaving a hole...

Im Focus: Quantum Sensors Decipher Magnetic Ordering in a New Semiconducting Material

For the first time, physicists have successfully imaged spiral magnetic ordering in a multiferroic material. These materials are considered highly promising candidates for future data storage media. The researchers were able to prove their findings using unique quantum sensors that were developed at Basel University and that can analyze electromagnetic fields on the nanometer scale. The results – obtained by scientists from the University of Basel’s Department of Physics, the Swiss Nanoscience Institute, the University of Montpellier and several laboratories from University Paris-Saclay – were recently published in the journal Nature.

Multiferroics are materials that simultaneously react to electric and magnetic fields. These two properties are rarely found together, and their combined...

All Focus news of the innovation-report >>>

Anzeige

Anzeige

Event News

“Lasers in Composites Symposium” in Aachen – from Science to Application

19.09.2017 | Event News

I-ESA 2018 – Call for Papers

12.09.2017 | Event News

EMBO at Basel Life, a new conference on current and emerging life science research

06.09.2017 | Event News

 
Latest News

Rainbow colors reveal cell history: Uncovering β-cell heterogeneity

22.09.2017 | Life Sciences

Penn first in world to treat patient with new radiation technology

22.09.2017 | Medical Engineering

Calculating quietness

22.09.2017 | Physics and Astronomy

VideoLinks
B2B-VideoLinks
More VideoLinks >>>