Forum for Science, Industry and Business

Sponsored by:     3M 
Search our Site:

 

Patient-specific stem cell lines - now a real possibility

28.11.2005


Mitosis in human embryonic stem (hES) cell cytoplast cybrid following fusion. A, B, C, D: Increasing appearance in background of red fluorescence [octamer binding transcription factor-4 (Oct-4)] surrounded by ring of green fluorescence [tumour rejection antigen-2-39 (TRA-2-39)], with no nucleoli being present. Blue fluorescence: DAPI, showing the chromosome location. Original magnification x40.


Immune rejection problems could affect any one of us. This unique research shows that producing individual patient cell lines for our own future needs is now something we might all want to consider.

Somatic cell nuclear transfer (SCNT) forms the basis for obtaining patient specific stem cells and with the presence of reprogramming factors in human embryonic stem (hES) cells, a method for replacing the nuclei of hES cells by somatic cell nuclei has been widely sought.

Nick Strelchenko et al. based at the Reproductive Genetics Institute in Chicago, USA, has now developed an original technique resulting in the first evidence of the complete replacement of the nuclei of hES cells by nuclei of somatic cells. Their paper Reprogramming of human somatic cells by embryonic stem cell cytoplast is accepted and was published online by Reproductive BioMedicine Online, www.rbmonline.com/Article/2071 on 18 November 2005. The final article will also be published in print in the January 2006 issue of the journal.



The new technique involves the fusion of different types of somatic cells with hES cells. The resulting ‘cybrids’ were shown to have the genotype of the donor somatic cells and the ‘stemness’ (the ability of the cells to divide, change throughout our lifetime, to provide cells that can become specialised and to replace those that die or are lost) of the recipient hES cells.

Nuclear reprogramming of somatic cells has been performed by fusion with ES cells, (Tada et al., 1997, 2001; Cowan et al., 2005). However, the resulting hybrid cells contained the nuclei of both somatic and hES cells (Cowan et al., 2005). This was due to the fact that the hES cells were not enucleated.

A key factor in this new research is that the hES cells were enucleated prior to their cytoplasts fusing with the somatic cell nuclei (Fig 1). Cell division then resulted in the establishment of cybrid cells (Fig 2) with typical hES cell morphology and ‘stemness’ shown by the presence of Oct -4 and TRA-2-39 confirming the replacement of the hES cell nuclei by the nuclei of somatic cells.

10-40 colonies were produced following 1-week culture of the resulting hybrid cells. These colonies contained cells with different karyotypes including the 46XY cybrid cells, representing a complete replacement of hES cell nuclei of donor somatic cells as well as 46,XXY and 92,XXYY cells representing hybrids between the donor and non-enucleated hES-cells. Fig 3 shows that the hES cell markers were positive in cybrid colonies which were cultured for many passages.

Whilst there still remains the problem of how to isolate the pure population of hES cell cybrid colonies and the extent to which the hES cell cybrids can be reprogrammed still needs to be determined, this research provides scientists with a new method of investigation and brings our ability to harness the therapeutic value of our own genetically identical stem cells significantly closer.

Yury Verlinsky | alfa
Further information:
http://www.rbmonline.com
http://www.rbmonline.com/Article/2071

More articles from Life Sciences:

nachricht Nanoparticle Exposure Can Awaken Dormant Viruses in the Lungs
16.01.2017 | Helmholtz Zentrum München - Deutsches Forschungszentrum für Gesundheit und Umwelt

nachricht Cholera bacteria infect more effectively with a simple twist of shape
13.01.2017 | Princeton University

All articles from Life Sciences >>>

The most recent press releases about innovation >>>

Die letzten 5 Focus-News des innovations-reports im Überblick:

Im Focus: Interfacial Superconductivity: Magnetic and superconducting order revealed simultaneously

Researchers from the University of Hamburg in Germany, in collaboration with colleagues from the University of Aarhus in Denmark, have synthesized a new superconducting material by growing a few layers of an antiferromagnetic transition-metal chalcogenide on a bismuth-based topological insulator, both being non-superconducting materials.

While superconductivity and magnetism are generally believed to be mutually exclusive, surprisingly, in this new material, superconducting correlations...

Im Focus: Studying fundamental particles in materials

Laser-driving of semimetals allows creating novel quasiparticle states within condensed matter systems and switching between different states on ultrafast time scales

Studying properties of fundamental particles in condensed matter systems is a promising approach to quantum field theory. Quasiparticles offer the opportunity...

Im Focus: Designing Architecture with Solar Building Envelopes

Among the general public, solar thermal energy is currently associated with dark blue, rectangular collectors on building roofs. Technologies are needed for aesthetically high quality architecture which offer the architect more room for manoeuvre when it comes to low- and plus-energy buildings. With the “ArKol” project, researchers at Fraunhofer ISE together with partners are currently developing two façade collectors for solar thermal energy generation, which permit a high degree of design flexibility: a strip collector for opaque façade sections and a solar thermal blind for transparent sections. The current state of the two developments will be presented at the BAU 2017 trade fair.

As part of the “ArKol – development of architecturally highly integrated façade collectors with heat pipes” project, Fraunhofer ISE together with its partners...

Im Focus: How to inflate a hardened concrete shell with a weight of 80 t

At TU Wien, an alternative for resource intensive formwork for the construction of concrete domes was developed. It is now used in a test dome for the Austrian Federal Railways Infrastructure (ÖBB Infrastruktur).

Concrete shells are efficient structures, but not very resource efficient. The formwork for the construction of concrete domes alone requires a high amount of...

Im Focus: Bacterial Pac Man molecule snaps at sugar

Many pathogens use certain sugar compounds from their host to help conceal themselves against the immune system. Scientists at the University of Bonn have now, in cooperation with researchers at the University of York in the United Kingdom, analyzed the dynamics of a bacterial molecule that is involved in this process. They demonstrate that the protein grabs onto the sugar molecule with a Pac Man-like chewing motion and holds it until it can be used. Their results could help design therapeutics that could make the protein poorer at grabbing and holding and hence compromise the pathogen in the host. The study has now been published in “Biophysical Journal”.

The cells of the mouth, nose and intestinal mucosa produce large quantities of a chemical called sialic acid. Many bacteria possess a special transport system...

All Focus news of the innovation-report >>>

Anzeige

Anzeige

Event News

12V, 48V, high-voltage – trends in E/E automotive architecture

10.01.2017 | Event News

2nd Conference on Non-Textual Information on 10 and 11 May 2017 in Hannover

09.01.2017 | Event News

Nothing will happen without batteries making it happen!

05.01.2017 | Event News

 
Latest News

Water - as the underlying driver of the Earth’s carbon cycle

17.01.2017 | Earth Sciences

Interfacial Superconductivity: Magnetic and superconducting order revealed simultaneously

17.01.2017 | Materials Sciences

Smart homes will “LISTEN” to your voice

17.01.2017 | Architecture and Construction

VideoLinks
B2B-VideoLinks
More VideoLinks >>>