Attempts to improve the chemotherapeutic efficacy and radiotherapy-sensitivity of the anticancer agent, gemcitabine, using gene therapy have yielded interesting results in preclinical glioma models presented at the 13th European Cancer Conference (ECCO).
Investigators took the enzyme that activates gemcitabine, deoxycytidine kinase (dCK) and inserted it into a viral carrier – Ad-dCK. In vitro assay cells from mice, rats and humans, and mice infected with glioma (tumours originating from the spinal cord or brain) were then infiltrated with this gene therapy. Assay cells were subsequently treated with gemcitabine and irradiated. Tumour-bearing mice received an intraperitoneal injection of gemcitabine followed by local tumour irradiation. As gemcitabine is an anticancer agent with established efficacy, use of gene therapy to increase its enzymatic activation was hypothesised to offer potential improvements in chemo- and radiotherapy efficacy.
In vitro findings from the three different experimental glioma varied considerably. In the G1261 mouse cellular assay, increased levels of dCK enzyme activity failed to increase gemcitabine toxicity - although gemcitabine itself had a minor radiosensitising effect. Conversely, in rat C6 and 9L glioma cells, elevated dCK levels were found to substantially improve both gemcitabine toxicity and the radiosensitising effect.
Enzyme with surprising dual function
24.01.2018 | Rheinische Friedrich-Wilhelms-Universität Bonn
Flexibility and arrangement - the interaction of ribonucleic acid and water
24.01.2018 | Forschungsverbund Berlin e.V.
Physicists have developed a technique based on optical microscopy that can be used to create images of atoms on the nanoscale. In particular, the new method allows the imaging of quantum dots in a semiconductor chip. Together with colleagues from the University of Bochum, scientists from the University of Basel’s Department of Physics and the Swiss Nanoscience Institute reported the findings in the journal Nature Photonics.
Microscopes allow us to see structures that are otherwise invisible to the human eye. However, conventional optical microscopes cannot be used to image...
On the way to an intelligent laboratory, physicists from Innsbruck and Vienna present an artificial agent that autonomously designs quantum experiments. In initial experiments, the system has independently (re)discovered experimental techniques that are nowadays standard in modern quantum optical laboratories. This shows how machines could play a more creative role in research in the future.
We carry smartphones in our pockets, the streets are dotted with semi-autonomous cars, but in the research laboratory experiments are still being designed by...
What enables electrons to be transferred swiftly, for example during photosynthesis? An interdisciplinary team of researchers has worked out the details of how...
For the first time, scientists have precisely measured the effective electrical charge of a single molecule in solution. This fundamental insight of an SNSF Professor could also pave the way for future medical diagnostics.
Electrical charge is one of the key properties that allows molecules to interact. Life itself depends on this phenomenon: many biological processes involve...
At the JEC World Composite Show in Paris in March 2018, the Fraunhofer Institute for Laser Technology ILT will be focusing on the latest trends and innovations in laser machining of composites. Among other things, researchers at the booth shared with the Aachen Center for Integrative Lightweight Production (AZL) will demonstrate how lasers can be used for joining, structuring, cutting and drilling composite materials.
No other industry has attracted as much public attention to composite materials as the automotive industry, which along with the aerospace industry is a driver...
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24.01.2018 | Health and Medicine