Rounding up wayward cells and particles on a microscope slide can be as difficult as corralling wild horses on the range, particularly if theres a need to separate a single individual from the group.
The images above show selective collection of live cells from a mixture of live and dead cells. In (a), the cells are randomly positioned. In (b) and (c), a series of optically projected concentric circles round up live cells, while dead cells (stained with Trypan blue dye) leak out through the dark gaps and are not collected. The optical pattern has a yellowish colour, while weak background scattered light results in a pinkish hue in the non-patterned areas. Section (d) shows the collection of live cells rounded up by the optoelectronic tweezer. (Courtesy of Wu Lab, UC Berkeley)
Shown is a schematic of the optoelectronic tweezer developed by UC Berkeley engineers. Liquid that contains microscopic particles is sandwiched between the top indium tin oxide (ITO) glass and the bottom photosensitive surface, made up of amorphous silicon (a-Si:H) and silicon nitride. The illumination source is a light-emitting diode operating at a wavelength of 625 nm. The optical images shown on the digital micromirror display (DMD) are focused onto the photosensitive surface and create the non-uniform electric field for manipulation of the particles. (Courtesy of Wu Lab, UC Berkeley)
But now, a new device developed by University of California, Berkeley, engineers, and dubbed an "optoelectronic tweezer," will enable researchers to easily manipulate large numbers of single cells and particles using optical images projected onto a glass slide coated with photoconductive materials.
"This is the first time a single light-emitting diode has been used to trap more than 10,000 microparticles at the same time," said Ming Wu, UC Berkeley professor of electrical engineering and computer sciences and principal investigator of the study. "Optoelectronic tweezers can produce instant microfluidic circuits without the need for sophisticated microfabrication techniques."
Sarah Yang | EurekAlert!
Antimicrobial substances identified in Komodo dragon blood
23.02.2017 | American Chemical Society
New Mechanisms of Gene Inactivation may prevent Aging and Cancer
23.02.2017 | Leibniz-Institut für Alternsforschung - Fritz-Lipmann-Institut e.V. (FLI)
In the field of nanoscience, an international team of physicists with participants from Konstanz has achieved a breakthrough in understanding heat transport
Cells need to repair damaged DNA in our genes to prevent the development of cancer and other diseases. Our cells therefore activate and send “repair-proteins”...
The Fraunhofer IWS Dresden and Technische Universität Dresden inaugurated their jointly operated Center for Additive Manufacturing Dresden (AMCD) with a festive ceremony on February 7, 2017. Scientists from various disciplines perform research on materials, additive manufacturing processes and innovative technologies, which build up components in a layer by layer process. This technology opens up new horizons for component design and combinations of functions. For example during fabrication, electrical conductors and sensors are already able to be additively manufactured into components. They provide information about stress conditions of a product during operation.
The 3D-printing technology, or additive manufacturing as it is often called, has long made the step out of scientific research laboratories into industrial...
Nature does amazing things with limited design materials. Grass, for example, can support its own weight, resist strong wind loads, and recover after being...
Nanometer-scale magnetic perforated grids could create new possibilities for computing. Together with international colleagues, scientists from the Helmholtz Zentrum Dresden-Rossendorf (HZDR) have shown how a cobalt grid can be reliably programmed at room temperature. In addition they discovered that for every hole ("antidot") three magnetic states can be configured. The results have been published in the journal "Scientific Reports".
Physicist Dr. Rantej Bali from the HZDR, together with scientists from Singapore and Australia, designed a special grid structure in a thin layer of cobalt in...
13.02.2017 | Event News
10.02.2017 | Event News
09.02.2017 | Event News
23.02.2017 | Physics and Astronomy
23.02.2017 | Earth Sciences
23.02.2017 | Life Sciences