Forum for Science, Industry and Business

Sponsored by:     3M 
Search our Site:

 

Essential genes for embryogenesis uncovered in major mouse mutagenesis project

15.07.2005


A team of scientists led by Cornell University’s Dr. John Schimenti reports today that an extraordinary number of genes are required for prenatal mammalian development. The researchers estimate that up to 19% of all genes are vital for embryogenesis in mice. Their study, which is one of the largest functional genomics projects described to date, is published in today’s online edition of the journal Genome Research.



In addition to the important implications for understanding mammalian developmental biology and the genetic basis for spontaneous abortions, the impressive scale of the study – an enormous logistical effort spanning the past six years – marks a major step forward in the functional annotation of the mouse genome.

"Due to the availability of whole-genome sequences, we are now in the powerful position of knowing the sequence identity of most genes, their locations in the genome, their expression patterns, and which proteins interact with one another," explains Schimenti. "However, identifying the functions of these genes is a much more difficult challenge. For most genes, direct experimentation in the context of a whole organism will be required."


In an effort to explore the functional content of 50 megabases of mouse chromosome 5, or approximately 2% of the genome, Schimenti and his colleagues generated over 1000 mouse pedigrees with random mutations using a chemical called N-ethyl-N-nitrosourea (ENU). They then screened offspring from these pedigrees for lethality and defects in behavior, fertility, fitness, hearing, and vision.

Of 42 mutations identified, an overwhelming majority – 34 mutations – resulted in embryonic lethality. The scientists attributed the lethality to defects in processes including cellular proliferation, gastrulation, cardiovascular function, craniofacial development, neural tube development, and placental development.

Combining their data with estimates from previous studies, the researchers predicted that between 13.7% and 19% of all mouse genes are necessary for embryonic viability. In comparison, research on other organisms has indicated that 6-10% of zebrafish genes and 7% of C. elegans genes are required for prenatal survival. "The higher percentage of embryonic lethal genes in mice may reflect an inherently more complex or sensitive process of development," report the scientists.

The project included major contributions from Schimenti’s collaborators at The University of Pennsylvania School of Medicine (Philadelphia, PA) and The Jackson Laboratory (Bar Harbor, ME), where the study was initiated.

Maria A. Smit | EurekAlert!
Further information:
http://www.cshl.edu

More articles from Life Sciences:

nachricht New risk factors for anxiety disorders
24.02.2017 | Julius-Maximilians-Universität Würzburg

nachricht Stingless bees have their nests protected by soldiers
24.02.2017 | Johannes Gutenberg-Universität Mainz

All articles from Life Sciences >>>

The most recent press releases about innovation >>>

Die letzten 5 Focus-News des innovations-reports im Überblick:

Im Focus: Breakthrough with a chain of gold atoms

In the field of nanoscience, an international team of physicists with participants from Konstanz has achieved a breakthrough in understanding heat transport

In the field of nanoscience, an international team of physicists with participants from Konstanz has achieved a breakthrough in understanding heat transport

Im Focus: DNA repair: a new letter in the cell alphabet

Results reveal how discoveries may be hidden in scientific “blind spots”

Cells need to repair damaged DNA in our genes to prevent the development of cancer and other diseases. Our cells therefore activate and send “repair-proteins”...

Im Focus: Dresdner scientists print tomorrow’s world

The Fraunhofer IWS Dresden and Technische Universität Dresden inaugurated their jointly operated Center for Additive Manufacturing Dresden (AMCD) with a festive ceremony on February 7, 2017. Scientists from various disciplines perform research on materials, additive manufacturing processes and innovative technologies, which build up components in a layer by layer process. This technology opens up new horizons for component design and combinations of functions. For example during fabrication, electrical conductors and sensors are already able to be additively manufactured into components. They provide information about stress conditions of a product during operation.

The 3D-printing technology, or additive manufacturing as it is often called, has long made the step out of scientific research laboratories into industrial...

Im Focus: Mimicking nature's cellular architectures via 3-D printing

Research offers new level of control over the structure of 3-D printed materials

Nature does amazing things with limited design materials. Grass, for example, can support its own weight, resist strong wind loads, and recover after being...

Im Focus: Three Magnetic States for Each Hole

Nanometer-scale magnetic perforated grids could create new possibilities for computing. Together with international colleagues, scientists from the Helmholtz Zentrum Dresden-Rossendorf (HZDR) have shown how a cobalt grid can be reliably programmed at room temperature. In addition they discovered that for every hole ("antidot") three magnetic states can be configured. The results have been published in the journal "Scientific Reports".

Physicist Dr. Rantej Bali from the HZDR, together with scientists from Singapore and Australia, designed a special grid structure in a thin layer of cobalt in...

All Focus news of the innovation-report >>>

Anzeige

Anzeige

Event News

Booth and panel discussion – The Lindau Nobel Laureate Meetings at the AAAS 2017 Annual Meeting

13.02.2017 | Event News

Complex Loading versus Hidden Reserves

10.02.2017 | Event News

International Conference on Crystal Growth in Freiburg

09.02.2017 | Event News

 
Latest News

Stingless bees have their nests protected by soldiers

24.02.2017 | Life Sciences

New risk factors for anxiety disorders

24.02.2017 | Life Sciences

MWC 2017: 5G Capital Berlin

24.02.2017 | Trade Fair News

VideoLinks
B2B-VideoLinks
More VideoLinks >>>