Forum for Science, Industry and Business

Sponsored by:     3M 
Search our Site:

 

Scientists from Spain, USA and Canada construct a physical map of the Drosophila buzzatii genome

30.06.2005


A useful tool for evolution studies



An international team of researchers led by the Universitat Autònoma de Barcelona Professor Alfredo Ruiz, has launched in this month’s issue of the journal Genome Research the first detailed physical map of the Drosophila buzzatii chromosomes. This fly species is used worldwide as a model for studies in evolutionary genetics.

The investigation involved two steps. First, a genomic library was produced. This library comprises more than 18.000 random fragments of the fly’s genome inserted within bacterial cells. Each bacterial clone carries one of these DNA fragments, about 150.000 nucleotides long, and can be multiplied in a laboratory culture to amplify the fly’s DNA.


The second step involved arranging the random fragments present in the library in the same order and position as they are in the intact chromosomes. This amounts to composing a puzzle with thousands of pieces. Two methods were used for this task. One of them is called “fingerprinting” and consists in cutting the original clones into smaller fragments and then sizing these fragments by electrophoresis. The computerized comparison of the fragments produced by different clones (their “fingerprints”) allows to determine which clones overlap and belong to the same chromosomal region. The second method required the hybridization of the genomic clones to the giant salivary gland chromosomes of the larvae, a technique known as “in situ hybridization” which allows locating the chromosomal position of each clone (see image).

The physical map of Drosophila buzzatii genome is one of the most detailed maps ever built in Drosophila. Its interest stems from the species Drosophila buzzatii, which is widely used in studies of genome evolution, ecological adaptation and speciation. Now, thanks to the genomic library and the physical map, the international research community will find it easier to compare the genomes of this and related species, and to assemble the reads when the genomes of these species are sequenced.

The research has been coordinated by Professor Alfredo Ruiz from the Departament of Genetics and Microbiology of the UAB (Spain), and the team included 13 other investigators from this university and from the Children’s Hospital Oakland Research Institute in Oakland, California (USA); the Genome Sciences Centre in Vancouver (Canada); and the Lawrence Berkeley National Laboratory, in Berkeley, California (USA).

Octavi López Coronado | alfa
Further information:
http://www.uab.es/

More articles from Life Sciences:

nachricht One step closer to reality
20.04.2018 | Max-Planck-Institut für Entwicklungsbiologie

nachricht The dark side of cichlid fish: from cannibal to caregiver
20.04.2018 | Veterinärmedizinische Universität Wien

All articles from Life Sciences >>>

The most recent press releases about innovation >>>

Die letzten 5 Focus-News des innovations-reports im Überblick:

Im Focus: Spider silk key to new bone-fixing composite

University of Connecticut researchers have created a biodegradable composite made of silk fibers that can be used to repair broken load-bearing bones without the complications sometimes presented by other materials.

Repairing major load-bearing bones such as those in the leg can be a long and uncomfortable process.

Im Focus: Writing and deleting magnets with lasers

Study published in the journal ACS Applied Materials & Interfaces is the outcome of an international effort that included teams from Dresden and Berlin in Germany, and the US.

Scientists at the Helmholtz-Zentrum Dresden-Rossendorf (HZDR) together with colleagues from the Helmholtz-Zentrum Berlin (HZB) and the University of Virginia...

Im Focus: Gamma-ray flashes from plasma filaments

Novel highly efficient and brilliant gamma-ray source: Based on model calculations, physicists of the Max PIanck Institute for Nuclear Physics in Heidelberg propose a novel method for an efficient high-brilliance gamma-ray source. A giant collimated gamma-ray pulse is generated from the interaction of a dense ultra-relativistic electron beam with a thin solid conductor. Energetic gamma-rays are copiously produced as the electron beam splits into filaments while propagating across the conductor. The resulting gamma-ray energy and flux enable novel experiments in nuclear and fundamental physics.

The typical wavelength of light interacting with an object of the microcosm scales with the size of this object. For atoms, this ranges from visible light to...

Im Focus: Basel researchers succeed in cultivating cartilage from stem cells

Stable joint cartilage can be produced from adult stem cells originating from bone marrow. This is made possible by inducing specific molecular processes occurring during embryonic cartilage formation, as researchers from the University and University Hospital of Basel report in the scientific journal PNAS.

Certain mesenchymal stem/stromal cells from the bone marrow of adults are considered extremely promising for skeletal tissue regeneration. These adult stem...

Im Focus: Like a wedge in a hinge

Researchers lay groundwork to tailor drugs for new targets in cancer therapy

In the fight against cancer, scientists are developing new drugs to hit tumor cells at so far unused weak points. Such a “sore spot” is the protein complex...

All Focus news of the innovation-report >>>

Anzeige

Anzeige

VideoLinks
Industry & Economy
Event News

Invitation to the upcoming "Current Topics in Bioinformatics: Big Data in Genomics and Medicine"

13.04.2018 | Event News

Unique scope of UV LED technologies and applications presented in Berlin: ICULTA-2018

12.04.2018 | Event News

IWOLIA: A conference bringing together German Industrie 4.0 and French Industrie du Futur

09.04.2018 | Event News

 
Latest News

Magnetic nano-imaging on a table top

20.04.2018 | Physics and Astronomy

Start of work for the world's largest electric truck

20.04.2018 | Interdisciplinary Research

Atoms may hum a tune from grand cosmic symphony

20.04.2018 | Physics and Astronomy

VideoLinks
Science & Research
Overview of more VideoLinks >>>