Scientists at ETH Zurich have set a world record in mass spectrometry by observing the largest ever mass-to-charge ratio of over 1 Mio Dalton (MDa) using a special mass spectrometer. The researchers in the team of Prof. Renato Zenobi had to overcome two obstacles, the intact vaporization and ionization of the sample, as well as the detection of the very large ions. Modern soft ionization methods allow giant molecules such as proteins or DNA to be brought into the gas phase. The ETH researchers employed desorption and ionization using a pulsed UV laser.
For mass separation, they used a time-of-flight mass spectrometer, which separates ions of different mass-to-charge ratio by their drift time through an evacuated fligh tube. The largest ions take the longest time, and are difficult or impossible to detect with conventional detectors, due to their small drift velocity. One possibility would be to produce multiply charged ions and detect them in a more accessible mass-to-charge ratio range. The ETH researchers chose a more direct and elegant way, by carrying out their measurements on an instrument developed by Comet AG of Switzerland equipped with a superconducting tunnel junction detector. In this way, simple and directly interpretable mass spectra were obtained from immunoglobuline M (ca. 1 MDa) and from von Willebrand factor, a group of proteins that play an important role in coagulation of blood (signals at 0.5, 1, 1.5 and 2 MDa).
Renato Zenobi | alfa
Decoding the genome's cryptic language
27.02.2017 | University of California - San Diego
New risk factors for anxiety disorders
24.02.2017 | Julius-Maximilians-Universität Würzburg
On January 15, 2009, Chesley B. Sullenberger was celebrated world-wide: after the two engines had failed due to bird strike, he and his flight crew succeeded after a glide flight with an Airbus A320 in ditching on the Hudson River. All 155 people on board were saved.
On January 15, 2009, Chesley B. Sullenberger was celebrated world-wide: after the two engines had failed due to bird strike, he and his flight crew succeeded...
In the field of nanoscience, an international team of physicists with participants from Konstanz has achieved a breakthrough in understanding heat transport
Cells need to repair damaged DNA in our genes to prevent the development of cancer and other diseases. Our cells therefore activate and send “repair-proteins”...
The Fraunhofer IWS Dresden and Technische Universität Dresden inaugurated their jointly operated Center for Additive Manufacturing Dresden (AMCD) with a festive ceremony on February 7, 2017. Scientists from various disciplines perform research on materials, additive manufacturing processes and innovative technologies, which build up components in a layer by layer process. This technology opens up new horizons for component design and combinations of functions. For example during fabrication, electrical conductors and sensors are already able to be additively manufactured into components. They provide information about stress conditions of a product during operation.
The 3D-printing technology, or additive manufacturing as it is often called, has long made the step out of scientific research laboratories into industrial...
Nature does amazing things with limited design materials. Grass, for example, can support its own weight, resist strong wind loads, and recover after being...
13.02.2017 | Event News
10.02.2017 | Event News
09.02.2017 | Event News
27.02.2017 | Materials Sciences
27.02.2017 | Interdisciplinary Research
27.02.2017 | Life Sciences