Forum for Science, Industry and Business

Sponsored by:     3M 
Search our Site:

 

UCR Chemist Part of Team Identifying New Areas of Gene Regulation

10.05.2005


Discovery of new location in a key protein that activates genetic activity aided with use of mass spectrometry at UCR

Researcher Kangling Zhang at the University of California, Riverside is part of a team that has discovered a new way that yeast governs genetic expression and repression, a finding that could be repeated in cells of other organisms.

Zhang, an academic coordinator at the Mass Spectrometry Facility of the Department of Chemistry at UCR, worked with Feng Xu and Michael Grunstein of the Department of Biological Chemistry at the David Geffen School of Medicine at UCLA on a paper titled Acetylation in Histone H3 Globular Domain Regulates Gene Expression in Yeast, which was published today in the journal Cell.



The paper focuses on observations of histones, the proteins that regulate genetic expression and form the major supporting structures housing the cell’s DNA. Histones interacting with each other form a ‘spool’ around which DNA is wrapped in the cell. Grunstein, one of the scientists in the current team, discovered in 1991 that sites of histone acetylation, a modification of the protein, play a fundamental role in the regulation of gene activation and repression.

The key findings of the current paper were the discovery of this acetylation at the core of the histone, rather than at the proteins’ ends, which are where most gene regulation is thought to take place. The team used mass spectrometry to show that acetylation at the core of the histone is associated with gene activation by attracting the protein string known as the SWI/SNI chromatin remodeling complex to the location of acetylation.

“In this paper, we used mass spectrometry to identify a novel acetylation site at the lysine 56 of yeast histone H3,” said Zhang, referring to the previously unknown location of a chemical opening to allow genetic transfers to occur. “We found acetylation at this site near the entry-exit points of the DNA superhelix as it wraps around the nucleosome is required for recruiting the nucleosome remodeling complex SWI/SNF and so regulates gene activity,” he said. “We show for the first time that a modification of a histone at the core of the protein, not the end, can regulate genes,” Grunstein added.

The mass spectrometry facility at the UCR’s Department of Chemistry and in the new Physical Sciences building provides super-high sensitivity for research in protein functions and in metabolic profiles of cells. The facility provides service and collaboration not limited to, protein separation, protein identification, sequencing, protein expression level quantification, as well as small molecule structural determination and metabolite identification.

Ricardo Duran | EurekAlert!
Further information:
http://www.ucr.edu

More articles from Life Sciences:

nachricht Immune Defense Without Collateral Damage
23.01.2017 | Universität Basel

nachricht The interactome of infected neural cells reveals new therapeutic targets for Zika
23.01.2017 | D'Or Institute for Research and Education

All articles from Life Sciences >>>

The most recent press releases about innovation >>>

Die letzten 5 Focus-News des innovations-reports im Überblick:

Im Focus: Quantum optical sensor for the first time tested in space – with a laser system from Berlin

For the first time ever, a cloud of ultra-cold atoms has been successfully created in space on board of a sounding rocket. The MAIUS mission demonstrates that quantum optical sensors can be operated even in harsh environments like space – a prerequi-site for finding answers to the most challenging questions of fundamental physics and an important innovation driver for everyday applications.

According to Albert Einstein's Equivalence Principle, all bodies are accelerated at the same rate by the Earth's gravity, regardless of their properties. This...

Im Focus: Traffic jam in empty space

New success for Konstanz physicists in studying the quantum vacuum

An important step towards a completely new experimental access to quantum physics has been made at University of Konstanz. The team of scientists headed by...

Im Focus: How gut bacteria can make us ill

HZI researchers decipher infection mechanisms of Yersinia and immune responses of the host

Yersiniae cause severe intestinal infections. Studies using Yersinia pseudotuberculosis as a model organism aim to elucidate the infection mechanisms of these...

Im Focus: Interfacial Superconductivity: Magnetic and superconducting order revealed simultaneously

Researchers from the University of Hamburg in Germany, in collaboration with colleagues from the University of Aarhus in Denmark, have synthesized a new superconducting material by growing a few layers of an antiferromagnetic transition-metal chalcogenide on a bismuth-based topological insulator, both being non-superconducting materials.

While superconductivity and magnetism are generally believed to be mutually exclusive, surprisingly, in this new material, superconducting correlations...

Im Focus: Studying fundamental particles in materials

Laser-driving of semimetals allows creating novel quasiparticle states within condensed matter systems and switching between different states on ultrafast time scales

Studying properties of fundamental particles in condensed matter systems is a promising approach to quantum field theory. Quasiparticles offer the opportunity...

All Focus news of the innovation-report >>>

Anzeige

Anzeige

Event News

Sustainable Water use in Agriculture in Eastern Europe and Central Asia

19.01.2017 | Event News

12V, 48V, high-voltage – trends in E/E automotive architecture

10.01.2017 | Event News

2nd Conference on Non-Textual Information on 10 and 11 May 2017 in Hannover

09.01.2017 | Event News

 
Latest News

Tracking movement of immune cells identifies key first steps in inflammatory arthritis

23.01.2017 | Health and Medicine

Electrocatalysis can advance green transition

23.01.2017 | Physics and Astronomy

New technology for mass-production of complex molded composite components

23.01.2017 | Process Engineering

VideoLinks
B2B-VideoLinks
More VideoLinks >>>