Forum for Science, Industry and Business

Sponsored by:     3M 
Search our Site:

 

New technique finds molecules necessary for cancer metastasis

05.04.2005


Provides unique drug targets to prevent spread

Tufts University researchers have identified several proteins on the surface of cancer cells that contribute to the cells’ ability to metastasize. When the researchers destroyed these particular proteins, the cancerous cells show a significant decrease in their ability to invade healthy cells – a finding that provides a new target for badly needed drugs. Although most cancer deaths occur from metastasis, not from the original cancer itself, no drug treatments are currently available specifically to prevent the spread of the cancer from the original site to other organs. The team also has discovered new roles related to the spread of cancer in two molecules known for other, non-cancer activities.

Dr. Daniel Jay presented the study on Sunday, April 3, at Experimental Biology 2005 in San Diego, as part of the scientific sessions of the American Society for Biochemistry and Molecular Biology.



The findings were made possible, says Dr. Jay, because he and his colleagues have developed a new Fluorform-Assisted Light Inactivation technology (FALI) that is a new generation of the Chromophore-Assisted Laser Inactivation (CALI) technique he created 17 years ago to inactivate specific proteins in living cells at precise times and locations. The researchers are able to destroy a specific protein, sparing all other proteins attached to the cell as well as the cell itself, by targeting the antibody to that specific cell. They tag the antibody with a dye that absorbs a specific wavelength of light. When the light is turned on (earlier technology required lasers; the new FALI technology needs only the light of a slide projector), the light energy absorbed by the dye in the antibody generates free radicals that destroy the specific protein bound by that antibody.

What gives the new FALI approach its power, says Dr. Jay, is its high throughput and its ability to couple with the large antibody libraries now available. Whereas the team used to look at one protein at a time, it now can rapidly scan thousands of the proteins associated with cancer cells, systematically "knocking out" one at a time and looking for those whose absence on the cell causes a significant decrease in invasiveness.

At the Experimental Biology 2005, Dr. Jay also describes two of the molecules identified by the FALI approach to have large implications for metastasis. Both were well known to scientists, but the Jay team is the first to recognize the roles they play in cancer. The first molecule, HSP90A, is a molecular chaperone that facilities the folding and activation of different proteins within the cells. The Jay laboratory was the first to recognize HSP90A also had a role outside the cell; it activates a particular matrix metalloprotease required for restructuring the surrounding matrix as cells move and invade.

The second molecule, the polio virus receptor CD155, has been recognized for decades as the pathway by which the polio virus is able to enter motor neurons in the nervous system. Dr. Jay and his team have found that the receptor also plays a role in how brain tumor cells move in the brain, spreading to healthy cells.

"Our current interest is cell motility related to the spread of cancer," says Dr. Jay, "but the speed and sensitivity of the FALI approach gives it wide applicability as a method to identify functionally important proteins in a variety of disease processes."

Coauthors of the presentation are Brenda Eustace, Takashi Sakurai, and Kevin Sloan. Funding for the study came from the National Cancer Institute and the Goldhirsh Foundation.

Sarah Goodwin | EurekAlert!
Further information:
http://www.faseb.org/

More articles from Life Sciences:

nachricht Researchers identify potentially druggable mutant p53 proteins that promote cancer growth
09.12.2016 | Cold Spring Harbor Laboratory

nachricht Plant-based substance boosts eyelash growth
09.12.2016 | Fraunhofer-Institut für Angewandte Polymerforschung IAP

All articles from Life Sciences >>>

The most recent press releases about innovation >>>

Die letzten 5 Focus-News des innovations-reports im Überblick:

Im Focus: Electron highway inside crystal

Physicists of the University of Würzburg have made an astonishing discovery in a specific type of topological insulators. The effect is due to the structure of the materials used. The researchers have now published their work in the journal Science.

Topological insulators are currently the hot topic in physics according to the newspaper Neue Zürcher Zeitung. Only a few weeks ago, their importance was...

Im Focus: Significantly more productivity in USP lasers

In recent years, lasers with ultrashort pulses (USP) down to the femtosecond range have become established on an industrial scale. They could advance some applications with the much-lauded “cold ablation” – if that meant they would then achieve more throughput. A new generation of process engineering that will address this issue in particular will be discussed at the “4th UKP Workshop – Ultrafast Laser Technology” in April 2017.

Even back in the 1990s, scientists were comparing materials processing with nanosecond, picosecond and femtosesecond pulses. The result was surprising:...

Im Focus: Shape matters when light meets atom

Mapping the interaction of a single atom with a single photon may inform design of quantum devices

Have you ever wondered how you see the world? Vision is about photons of light, which are packets of energy, interacting with the atoms or molecules in what...

Im Focus: Novel silicon etching technique crafts 3-D gradient refractive index micro-optics

A multi-institutional research collaboration has created a novel approach for fabricating three-dimensional micro-optics through the shape-defined formation of porous silicon (PSi), with broad impacts in integrated optoelectronics, imaging, and photovoltaics.

Working with colleagues at Stanford and The Dow Chemical Company, researchers at the University of Illinois at Urbana-Champaign fabricated 3-D birefringent...

Im Focus: Quantum Particles Form Droplets

In experiments with magnetic atoms conducted at extremely low temperatures, scientists have demonstrated a unique phase of matter: The atoms form a new type of quantum liquid or quantum droplet state. These so called quantum droplets may preserve their form in absence of external confinement because of quantum effects. The joint team of experimental physicists from Innsbruck and theoretical physicists from Hannover report on their findings in the journal Physical Review X.

“Our Quantum droplets are in the gas phase but they still drop like a rock,” explains experimental physicist Francesca Ferlaino when talking about the...

All Focus news of the innovation-report >>>

Anzeige

Anzeige

Event News

ICTM Conference 2017: Production technology for turbomachine manufacturing of the future

16.11.2016 | Event News

Innovation Day Laser Technology – Laser Additive Manufacturing

01.11.2016 | Event News

#IC2S2: When Social Science meets Computer Science - GESIS will host the IC2S2 conference 2017

14.10.2016 | Event News

 
Latest News

Researchers identify potentially druggable mutant p53 proteins that promote cancer growth

09.12.2016 | Life Sciences

Scientists produce a new roadmap for guiding development & conservation in the Amazon

09.12.2016 | Ecology, The Environment and Conservation

Satellites, airport visibility readings shed light on troops' exposure to air pollution

09.12.2016 | Health and Medicine

VideoLinks
B2B-VideoLinks
More VideoLinks >>>