Forum for Science, Industry and Business

Sponsored by:     3M 
Search our Site:

 

New technique finds molecules necessary for cancer metastasis

05.04.2005


Provides unique drug targets to prevent spread

Tufts University researchers have identified several proteins on the surface of cancer cells that contribute to the cells’ ability to metastasize. When the researchers destroyed these particular proteins, the cancerous cells show a significant decrease in their ability to invade healthy cells – a finding that provides a new target for badly needed drugs. Although most cancer deaths occur from metastasis, not from the original cancer itself, no drug treatments are currently available specifically to prevent the spread of the cancer from the original site to other organs. The team also has discovered new roles related to the spread of cancer in two molecules known for other, non-cancer activities.

Dr. Daniel Jay presented the study on Sunday, April 3, at Experimental Biology 2005 in San Diego, as part of the scientific sessions of the American Society for Biochemistry and Molecular Biology.



The findings were made possible, says Dr. Jay, because he and his colleagues have developed a new Fluorform-Assisted Light Inactivation technology (FALI) that is a new generation of the Chromophore-Assisted Laser Inactivation (CALI) technique he created 17 years ago to inactivate specific proteins in living cells at precise times and locations. The researchers are able to destroy a specific protein, sparing all other proteins attached to the cell as well as the cell itself, by targeting the antibody to that specific cell. They tag the antibody with a dye that absorbs a specific wavelength of light. When the light is turned on (earlier technology required lasers; the new FALI technology needs only the light of a slide projector), the light energy absorbed by the dye in the antibody generates free radicals that destroy the specific protein bound by that antibody.

What gives the new FALI approach its power, says Dr. Jay, is its high throughput and its ability to couple with the large antibody libraries now available. Whereas the team used to look at one protein at a time, it now can rapidly scan thousands of the proteins associated with cancer cells, systematically "knocking out" one at a time and looking for those whose absence on the cell causes a significant decrease in invasiveness.

At the Experimental Biology 2005, Dr. Jay also describes two of the molecules identified by the FALI approach to have large implications for metastasis. Both were well known to scientists, but the Jay team is the first to recognize the roles they play in cancer. The first molecule, HSP90A, is a molecular chaperone that facilities the folding and activation of different proteins within the cells. The Jay laboratory was the first to recognize HSP90A also had a role outside the cell; it activates a particular matrix metalloprotease required for restructuring the surrounding matrix as cells move and invade.

The second molecule, the polio virus receptor CD155, has been recognized for decades as the pathway by which the polio virus is able to enter motor neurons in the nervous system. Dr. Jay and his team have found that the receptor also plays a role in how brain tumor cells move in the brain, spreading to healthy cells.

"Our current interest is cell motility related to the spread of cancer," says Dr. Jay, "but the speed and sensitivity of the FALI approach gives it wide applicability as a method to identify functionally important proteins in a variety of disease processes."

Coauthors of the presentation are Brenda Eustace, Takashi Sakurai, and Kevin Sloan. Funding for the study came from the National Cancer Institute and the Goldhirsh Foundation.

Sarah Goodwin | EurekAlert!
Further information:
http://www.faseb.org/

More articles from Life Sciences:

nachricht Could this protein protect people against coronary artery disease?
17.11.2017 | University of North Carolina Health Care

nachricht Microbial resident enables beetles to feed on a leafy diet
17.11.2017 | Max-Planck-Institut für chemische Ökologie

All articles from Life Sciences >>>

The most recent press releases about innovation >>>

Die letzten 5 Focus-News des innovations-reports im Überblick:

Im Focus: A “cosmic snake” reveals the structure of remote galaxies

The formation of stars in distant galaxies is still largely unexplored. For the first time, astron-omers at the University of Geneva have now been able to closely observe a star system six billion light-years away. In doing so, they are confirming earlier simulations made by the University of Zurich. One special effect is made possible by the multiple reflections of images that run through the cosmos like a snake.

Today, astronomers have a pretty accurate idea of how stars were formed in the recent cosmic past. But do these laws also apply to older galaxies? For around a...

Im Focus: Visual intelligence is not the same as IQ

Just because someone is smart and well-motivated doesn't mean he or she can learn the visual skills needed to excel at tasks like matching fingerprints, interpreting medical X-rays, keeping track of aircraft on radar displays or forensic face matching.

That is the implication of a new study which shows for the first time that there is a broad range of differences in people's visual ability and that these...

Im Focus: Novel Nano-CT device creates high-resolution 3D-X-rays of tiny velvet worm legs

Computer Tomography (CT) is a standard procedure in hospitals, but so far, the technology has not been suitable for imaging extremely small objects. In PNAS, a team from the Technical University of Munich (TUM) describes a Nano-CT device that creates three-dimensional x-ray images at resolutions up to 100 nanometers. The first test application: Together with colleagues from the University of Kassel and Helmholtz-Zentrum Geesthacht the researchers analyzed the locomotory system of a velvet worm.

During a CT analysis, the object under investigation is x-rayed and a detector measures the respective amount of radiation absorbed from various angles....

Im Focus: Researchers Develop Data Bus for Quantum Computer

The quantum world is fragile; error correction codes are needed to protect the information stored in a quantum object from the deteriorating effects of noise. Quantum physicists in Innsbruck have developed a protocol to pass quantum information between differently encoded building blocks of a future quantum computer, such as processors and memories. Scientists may use this protocol in the future to build a data bus for quantum computers. The researchers have published their work in the journal Nature Communications.

Future quantum computers will be able to solve problems where conventional computers fail today. We are still far away from any large-scale implementation,...

Im Focus: Wrinkles give heat a jolt in pillared graphene

Rice University researchers test 3-D carbon nanostructures' thermal transport abilities

Pillared graphene would transfer heat better if the theoretical material had a few asymmetric junctions that caused wrinkles, according to Rice University...

All Focus news of the innovation-report >>>

Anzeige

Anzeige

Event News

Ecology Across Borders: International conference brings together 1,500 ecologists

15.11.2017 | Event News

Road into laboratory: Users discuss biaxial fatigue-testing for car and truck wheel

15.11.2017 | Event News

#Berlin5GWeek: The right network for Industry 4.0

30.10.2017 | Event News

 
Latest News

NASA detects solar flare pulses at Sun and Earth

17.11.2017 | Physics and Astronomy

NIST scientists discover how to switch liver cancer cell growth from 2-D to 3-D structures

17.11.2017 | Health and Medicine

The importance of biodiversity in forests could increase due to climate change

17.11.2017 | Studies and Analyses

VideoLinks
B2B-VideoLinks
More VideoLinks >>>