Forum for Science, Industry and Business

Sponsored by:     3M 
Search our Site:

 

Components in grapes inhibit enzyme key to proliferation of cancer cells

31.03.2005


Components in grapes, including some newly identified ones, work together to dramatically inhibit an enzyme crucial to the proliferation of cancer cells, say scientists at the University of Illinois at Urbana-Champaign.



The work -- done using advanced molecular tools with grape-cell cultures and the target enzyme for new anti-cancer strategies -- helps to identify which flavonoids in grapes and red wine are most responsible for anti-cancer qualities, said Mary Ann Lila, a professor in the department of natural resources and environmental sciences.

Flavonoids are a group of organic compounds that include numerous water-soluble plant pigments responsible for colors. They are more abundant in red than in white grapes.


The Journal of Agricultural and Food Chemistry has posted the Illinois study online ahead of regular publication. The study details a dozen newly discovered constituents in grape-cell culture extracts and how some of them work synergistically against an enzyme known as human DNA topoisomerase II. The enzyme is necessary for the spread of cancer and commonly used in cancer research to screen plant chemicals.

"The findings add to the argument for eating whole foods," said Elvira Gonzalez de Mejia, a professor in the department of food science and human nutrition. "It’s very clear that the synergy is critical. When a cell becomes malignant that enzyme is expressed 300 times more than in a normal cell. If we can find a compound or mixture of compounds that can reduce the activity of that enzyme, the cancerous cells will die."

The synergistic activity involves specific phytochemicals from the proanthocyanidin and anthocyanin classes of the varied flavonoid family. They worked more effectively against the enzyme than do the previously identified flavonoids quercetin and resveratrol. Alone, the individual compenents had less effect on the enzyme.

"We definitely had very potent activity against the particular antibody system we were using, which was that of the critical proliferation stage of carcinogenesis," Lila said. "In our subsequent studies now under way in animal models, we are getting direct evidence that these components in grapes work synergistically in fighting cancer. They have to work together to obtain the potency that works."

The researchers are tracking where specially radiolabeled flavonoids congregate in rats, in a project funded by the U.S. Department of Agriculture. "We are finding that these flavonoids are very bioavailable," de Mejia said. "By eating the fruit, we know that the bioactive component involved goes into your bloodstream and relocates to other regions. Before now, we didn’t really know that."

Lila, de Mejia and co-author Jeong-Youn Jo, a doctoral student in Lila’s lab, produced the grape-cell cultures they tested from red-grape plants specifically bred for their pigmentation and provided by Cornell University researchers.

Using vegetative samples of the plants, rather than the fruit itself, the Illinois team was able to quickly produce the whole range of grape flavonoids in greater quantity. The researchers then extracted individual flavonoids intact. Their analytic work involved the use of reversed phase high-performance liquid chromatography and LC-electrospray ionization (ESI)/mass spectrometry to profile the most bioactive components.

Eventually, Lila said, researchers may be able to determine reasonable dosages for therapeutic consumption of flavonoid-rich grapes. Supplements containing specific flavonoids probably won’t result in desired benefits, de Mejia said, because complementary components required for synergistic activity may be missing.

"Some of the compounds we identified have not been reported in cell culture and grapes," de Mejia said. "Some have high inhibitory activity in the promotion and progression stages of cancer and have a high probability to work against the disease."

Jim Barlow | EurekAlert!
Further information:
http://www.uiuc.edu

More articles from Life Sciences:

nachricht Ion treatments for cardiac arrhythmia — Non-invasive alternative to catheter-based surgery
20.01.2017 | GSI Helmholtzzentrum für Schwerionenforschung GmbH

nachricht Seeking structure with metagenome sequences
20.01.2017 | DOE/Joint Genome Institute

All articles from Life Sciences >>>

The most recent press releases about innovation >>>

Die letzten 5 Focus-News des innovations-reports im Überblick:

Im Focus: Traffic jam in empty space

New success for Konstanz physicists in studying the quantum vacuum

An important step towards a completely new experimental access to quantum physics has been made at University of Konstanz. The team of scientists headed by...

Im Focus: How gut bacteria can make us ill

HZI researchers decipher infection mechanisms of Yersinia and immune responses of the host

Yersiniae cause severe intestinal infections. Studies using Yersinia pseudotuberculosis as a model organism aim to elucidate the infection mechanisms of these...

Im Focus: Interfacial Superconductivity: Magnetic and superconducting order revealed simultaneously

Researchers from the University of Hamburg in Germany, in collaboration with colleagues from the University of Aarhus in Denmark, have synthesized a new superconducting material by growing a few layers of an antiferromagnetic transition-metal chalcogenide on a bismuth-based topological insulator, both being non-superconducting materials.

While superconductivity and magnetism are generally believed to be mutually exclusive, surprisingly, in this new material, superconducting correlations...

Im Focus: Studying fundamental particles in materials

Laser-driving of semimetals allows creating novel quasiparticle states within condensed matter systems and switching between different states on ultrafast time scales

Studying properties of fundamental particles in condensed matter systems is a promising approach to quantum field theory. Quasiparticles offer the opportunity...

Im Focus: Designing Architecture with Solar Building Envelopes

Among the general public, solar thermal energy is currently associated with dark blue, rectangular collectors on building roofs. Technologies are needed for aesthetically high quality architecture which offer the architect more room for manoeuvre when it comes to low- and plus-energy buildings. With the “ArKol” project, researchers at Fraunhofer ISE together with partners are currently developing two façade collectors for solar thermal energy generation, which permit a high degree of design flexibility: a strip collector for opaque façade sections and a solar thermal blind for transparent sections. The current state of the two developments will be presented at the BAU 2017 trade fair.

As part of the “ArKol – development of architecturally highly integrated façade collectors with heat pipes” project, Fraunhofer ISE together with its partners...

All Focus news of the innovation-report >>>

Anzeige

Anzeige

Event News

Sustainable Water use in Agriculture in Eastern Europe and Central Asia

19.01.2017 | Event News

12V, 48V, high-voltage – trends in E/E automotive architecture

10.01.2017 | Event News

2nd Conference on Non-Textual Information on 10 and 11 May 2017 in Hannover

09.01.2017 | Event News

 
Latest News

Helmholtz International Fellow Award for Sarah Amalia Teichmann

20.01.2017 | Awards Funding

An innovative high-performance material: biofibers made from green lacewing silk

20.01.2017 | Materials Sciences

Ion treatments for cardiac arrhythmia — Non-invasive alternative to catheter-based surgery

20.01.2017 | Life Sciences

VideoLinks
B2B-VideoLinks
More VideoLinks >>>