Transplanted bone marrow cells reduce liver fibrosis in mice
Transplanted bone marrow cells can reduce carbon tetrachloride-induced liver fibrosis in mice and significantly improve their survival rates, according to a new study published in the December 2004 issue of Hepatology, the official journal of the American Association for the Study of Liver Diseases (AASLD). Published by John Wiley & Sons, Inc., Hepatology is available online via Wiley InterScience at http://www.interscience.wiley.com/journal/hepatology.
Previous reports have shown that bone marrow cells can differentiate into a number of other types of cells, including liver cells, which could help patients with liver cirrhosis and chronic liver failure. To study this possibility, researchers, led by Isao Sakaida, M.D. of Japans Yamaguchi University, studied the effect of transplanted bone marrow cells on mice with liver fibrosis.
The researchers first caused liver fibrosis in the mice by injecting them with carbon tetrachloride (CCI4) twice a week for four weeks. They then divided the mice into two groups and treated one with green fluorescent protein-positive blood marrow cells. They treated the control group with saline. All mice continued to be treated with carbon tetrachloride. After 1, 2, 3 or 4 weeks, the researchers assessed the extent of liver fibrosis in the mice. To measure survival rates, 15 mice from the experimental group and 15 from the control group were then treated with carbon tetrachloride for an additional 25 weeks.
After five weeks of treatment with carbon tetrachloride, the researchers detected liver fibrosis in the mice. Just one week after blood marrow cell transplantation, they found evidence of those cells in the liver, with more appearing as the weeks passed. "Surprisingly," the researchers report, "four weeks later, the blood marrow cell-transplanted liver clearly showed reduction of liver fibrosis compared with the liver treated with CCI4 alone at 8 weeks."
Furthermore, the mice that received blood marrow cell transplants along with continuing carbon tetrachloride treatments showed a gradually increased serum albumin level and had significantly improved survival rates compared with mice that only received carbon tetrachloride treatments.
The transplanted blood marrow cells degraded collagen fibers and reduced liver fibrosis, exhibiting strong expression of matrix metalloproteinases (MMPs), especially MMP-9. "The reason for the strong expression of MMP-9 is still unknown," report the authors, but report that it was somehow related to the migration of the blood marrow cells to the inflammatory liver and to those cells degradation of the liver fibrosis.
"The present study clearly indicates that this subpopulation of blood marrow cells is responsible for the resolution of liver fibrosis induced by CCI4 treatment," the authors conclude, and "introduces a new concept for the treatment of liver fibrosis."
David Greenberg | EurekAlert!
The most recent press releases about innovation >>>
Die letzten 5 Focus-News des innovations-reports im Überblick:
Using ultrafast flashes of laser and x-ray radiation, scientists at the Max Planck Institute of Quantum Optics (Garching, Germany) took snapshots of the briefest electron motion inside a solid material to date. The electron motion lasted only 750 billionths of the billionth of a second before it fainted, setting a new record of human capability to capture ultrafast processes inside solids!
When x-rays shine onto solid materials or large molecules, an electron is pushed away from its original place near the nucleus of the atom, leaving a hole...
For the first time, physicists have successfully imaged spiral magnetic ordering in a multiferroic material. These materials are considered highly promising candidates for future data storage media. The researchers were able to prove their findings using unique quantum sensors that were developed at Basel University and that can analyze electromagnetic fields on the nanometer scale. The results – obtained by scientists from the University of Basel’s Department of Physics, the Swiss Nanoscience Institute, the University of Montpellier and several laboratories from University Paris-Saclay – were recently published in the journal Nature.
Multiferroics are materials that simultaneously react to electric and magnetic fields. These two properties are rarely found together, and their combined...
MBM ScienceBridge GmbH successfully negotiated a license agreement between University Medical Center Göttingen (UMG) and the biotech company Tissue Systems Holding GmbH about commercial use of a multi-well tissue plate for automated and reliable tissue engineering & drug testing.
MBM ScienceBridge GmbH successfully negotiated a license agreement between University Medical Center Göttingen (UMG) and the biotech company Tissue Systems...
HZI researchers pave the way for new agents that render hospital pathogens mute
Pathogenic bacteria are becoming resistant to common antibiotics to an ever increasing degree. One of the most difficult germs is Pseudomonas aeruginosa, a...
Scientists from the MPI for Chemical Energy Conversion report in the first issue of the new journal JOULE.
Cell Press has just released the first issue of Joule, a new journal dedicated to sustainable energy research. In this issue James Birrell, Olaf Rüdiger,...