Four years ago in Science, Stuart Kim, a Stanford University developmental biologist, made the case for describing the broad strokes of a complex physiological process before defining its mechanisms. "A powerful, top-down, holistic approach," he wrote, "is to identify all of the components of a particular cellular process, so that one can define the global picture first and then use it as a framework to understand how the individual components of the process fit together." To get a broad view of gene expression in the developing nematode, Kims lab turned to DNA microarray technology. In a new study, Kim and colleagues apply this same approach to the decidedly more complex problem of human aging and "present a molecular portrait" of the aging kidney.
Genome-wide analysis of aging in the human kidney. Photo illustration by Rebecca Sonu and Emily Crane.
Kidneys came from 74 patients, ranging in age from 27 to 92. Samples were extracted from donated kidneys or "meticulously harvested" from kidneys with localized disease to ensure only normal tissue was taken. Samples were obtained from two structures that are critical to kidney function: the renal cortex, which filters plasma, and the medulla, which alters urine composition to maintain fluid balance. Both deteriorate with age.
Kim and colleagues then used DNA microarrays to determine the activity of every gene in all the kidney samples. Of 33,000 known human genes on the microarray, 985 showed age-related changes.
Paul Ocampo | EurekAlert!
Signaling Pathways to the Nucleus
19.03.2018 | Albert-Ludwigs-Universität Freiburg im Breisgau
In monogamous species, a compatible partner is more important than an ornamented one
19.03.2018 | Max-Planck-Institut für Ornithologie
For the first time, an interdisciplinary team from the University of Basel has succeeded in integrating artificial organelles into the cells of live zebrafish embryos. This innovative approach using artificial organelles as cellular implants offers new potential in treating a range of diseases, as the authors report in an article published in Nature Communications.
In the cells of higher organisms, organelles such as the nucleus or mitochondria perform a range of complex functions necessary for life. In the networks of...
Animal photoreceptors capture light with photopigments. Researchers from the University of Göttingen have now discovered that these photopigments fulfill an...
On 15 March, the AWI research aeroplane Polar 5 will depart for Greenland. Concentrating on the furthest northeast region of the island, an international team...
The world’s second-largest ice shelf was the destination for a Polarstern expedition that ended in Punta Arenas, Chile on 14th March 2018. Oceanographers from...
At the 2018 ILA Berlin Air Show from April 25–29, the Fraunhofer Institute for Laser Technology ILT is showcasing extreme high-speed Laser Material Deposition (EHLA): A video documents how for metal components that are highly loaded, EHLA has already proved itself as an alternative to hard chrome plating, which is now allowed only under special conditions.
When the EU restricted the use of hexavalent chromium compounds to special applications requiring authorization, the move prompted a rethink in the surface...
19.03.2018 | Event News
16.03.2018 | Event News
13.03.2018 | Event News
19.03.2018 | Physics and Astronomy
19.03.2018 | Materials Sciences
19.03.2018 | Event News