Because 60 percent of yeast genes have at least one clearly identifiable human counterpart, the advance, described in the Nov. 5 issue of Molecular Cell, should speed advances in understanding human gene and protein functions, as well as improve the reliability of what scientists think they know about this extremely useful microorganism. Eventually the work with yeast could reveal particular gene interactions that could become targets for therapies to fight cancers or fungal infections, say the researchers.
The toolkit, a combination of techniques developed by the Hopkins researchers and others, starts with a collection of almost 6,000 yeast strains, each missing a different gene, and allows researchers to identify genes whose coupled elimination kills the yeast. Many laboratories are already using the "single knock-out" yeast collections, but postdoctoral fellow Xuewen Pan, Ph.D., found a way to protect the genetic integrity of the collection so that repeated experiments will provide the same results, regardless of when and where the experiments are conducted.
"Everyone in the yeast community has been using their own batch of yeast mutants, but the slow-growing mutants gradually accumulate extra genetic changes so they can grow faster," says Jef Boeke, Ph.D., professor of molecular biology and genetics and director of the HighThroughput Biology (HiT) Center in Hopkins Institute for Basic Biomedical Sciences. "This potential for genetic impurity means that one persons batch of yeast is no longer exactly the same as someone elses. We went back to the original stocks of yeast mutants, in certain cases, so we know exactly what we have."
Joanna Downer | EurekAlert!
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Whether you call it effervescent, fizzy, or sparkling, carbonated water is making a comeback as a beverage. Aside from quenching thirst, researchers at the University of Illinois at Urbana-Champaign have discovered a new use for these "bubbly" concoctions that will have major impact on the manufacturer of the world's thinnest, flattest, and one most useful materials -- graphene.
As graphene's popularity grows as an advanced "wonder" material, the speed and quality at which it can be manufactured will be paramount. With that in mind,...
Physicists at the University of Bonn have managed to create optical hollows and more complex patterns into which the light of a Bose-Einstein condensate flows. The creation of such highly low-loss structures for light is a prerequisite for complex light circuits, such as for quantum information processing for a new generation of computers. The researchers are now presenting their results in the journal Nature Photonics.
Light particles (photons) occur as tiny, indivisible portions. Many thousands of these light portions can be merged to form a single super-photon if they are...
For the first time, scientists have shown that circular RNA is linked to brain function. When a RNA molecule called Cdr1as was deleted from the genome of mice, the animals had problems filtering out unnecessary information – like patients suffering from neuropsychiatric disorders.
While hundreds of circular RNAs (circRNAs) are abundant in mammalian brains, one big question has remained unanswered: What are they actually good for? In the...
An experimental small satellite has successfully collected and delivered data on a key measurement for predicting changes in Earth's climate.
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A study led by scientists of the Max Planck Institute for the Structure and Dynamics of Matter (MPSD) at the Center for Free-Electron Laser Science in Hamburg presents evidence of the coexistence of superconductivity and “charge-density-waves” in compounds of the poorly-studied family of bismuthates. This observation opens up new perspectives for a deeper understanding of the phenomenon of high-temperature superconductivity, a topic which is at the core of condensed matter research since more than 30 years. The paper by Nicoletti et al has been published in the PNAS.
Since the beginning of the 20th century, superconductivity had been observed in some metals at temperatures only a few degrees above the absolute zero (minus...
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