Because 60 percent of yeast genes have at least one clearly identifiable human counterpart, the advance, described in the Nov. 5 issue of Molecular Cell, should speed advances in understanding human gene and protein functions, as well as improve the reliability of what scientists think they know about this extremely useful microorganism. Eventually the work with yeast could reveal particular gene interactions that could become targets for therapies to fight cancers or fungal infections, say the researchers.
The toolkit, a combination of techniques developed by the Hopkins researchers and others, starts with a collection of almost 6,000 yeast strains, each missing a different gene, and allows researchers to identify genes whose coupled elimination kills the yeast. Many laboratories are already using the "single knock-out" yeast collections, but postdoctoral fellow Xuewen Pan, Ph.D., found a way to protect the genetic integrity of the collection so that repeated experiments will provide the same results, regardless of when and where the experiments are conducted.
"Everyone in the yeast community has been using their own batch of yeast mutants, but the slow-growing mutants gradually accumulate extra genetic changes so they can grow faster," says Jef Boeke, Ph.D., professor of molecular biology and genetics and director of the HighThroughput Biology (HiT) Center in Hopkins Institute for Basic Biomedical Sciences. "This potential for genetic impurity means that one persons batch of yeast is no longer exactly the same as someone elses. We went back to the original stocks of yeast mutants, in certain cases, so we know exactly what we have."
Joanna Downer | EurekAlert!
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The Institute of Semiconductor Technology and the Institute of Physical and Theoretical Chemistry, both members of the Laboratory for Emerging Nanometrology (LENA), at Technische Universität Braunschweig are partners in a new European research project entitled ChipScope, which aims to develop a completely new and extremely small optical microscope capable of observing the interior of living cells in real time. A consortium of 7 partners from 5 countries will tackle this issue with very ambitious objectives during a four-year research program.
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Researchers at the Goethe University Frankfurt, together with partners from the University of Tübingen in Germany and Queen Mary University as well as Francis Crick Institute from London (UK) have developed a novel technology to decipher the secret ubiquitin code.
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Silicon nanosheets are thin, two-dimensional layers with exceptional optoelectronic properties very similar to those of graphene. Albeit, the nanosheets are less stable. Now researchers at the Technical University of Munich (TUM) have, for the first time ever, produced a composite material combining silicon nanosheets and a polymer that is both UV-resistant and easy to process. This brings the scientists a significant step closer to industrial applications like flexible displays and photosensors.
Silicon nanosheets are thin, two-dimensional layers with exceptional optoelectronic properties very similar to those of graphene. Albeit, the nanosheets are...
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