Forum for Science, Industry and Business

Sponsored by:     3M 
Search our Site:


New quick test for drug-resistant strains of gonorrhea


Potential antimicrobial resistance in the bacteria that cause gonorrhea can be detected without culturing the organism, thanks to a rapid test developed by researchers at Johns Hopkins.

Key to the usefulness of the new test is that it does not require collection, culture or testing of the bacteria themselves - called Neisseria gonorrhea. Instead, the genes linked to resistance can be identified in urine samples or in leftover products from other commonly used diagnostic techniques, the Hopkins team reports.

This new application of probe technology should help public health officials study the spread of antibiotic-resistant gonorrhea by simplifying analysis of samples that cannot be used for culturing organisms, the Hopkins group added.

The Hopkins team developed the test by using an existing diagnostic technology called nucleic acid amplification tests (NAATs). These are FDA-approved tests that detect gonorrhea DNA in urine samples. Using leftover DNA from NAATs, the team performed a polymerase chain reaction (PCR) to make copies of genes linked to resistance. They then performed a melt curve analysis to detect mutations in these areas. In melt curve analysis, a short DNA sequence that matches the bacterial sequence in question is labeled with a fluorescent dye. The labeled sequence, called a probe, emits light only when bound to its target.

The probe and the DNA copies produced by PCR are dissolved together and the solution is slowly heated and cooled. This lets the probe bind to its target. Then the solution is slowly heated until the probe is melted from its target. A special instrument measures the temperature at which the probe melts from the DNA by identifying the temperature at which the probe ceases to emit light. The probe melts at a lower temperature if the target contains mutations and at a higher temperature if there are no mutations in that region of the gene. As a result, the lower melt temperature indicates potential resistance.

Rapid detection and characterization of gonococcal resistance determinants in NAAT samples. Julie Giles, Justin Hardick, Jeffrey Yuenger, Charlotte Gaydos, Jonathan Zenilman

David March | EurekAlert!
Further information:

More articles from Life Sciences:

nachricht Don't Give the Slightest Chance to Toxic Elements in Medicinal Products
23.03.2018 | Physikalisch-Technische Bundesanstalt (PTB)

nachricht North and South Cooperation to Combat Tuberculosis
22.03.2018 | Universität Zürich

All articles from Life Sciences >>>

The most recent press releases about innovation >>>

Die letzten 5 Focus-News des innovations-reports im Überblick:

Im Focus: Space observation with radar to secure Germany's space infrastructure

Satellites in near-Earth orbit are at risk due to the steady increase in space debris. But their mission in the areas of telecommunications, navigation or weather forecasts is essential for society. Fraunhofer FHR therefore develops radar-based systems which allow the detection, tracking and cataloging of even the smallest particles of debris. Satellite operators who have access to our data are in a better position to plan evasive maneuvers and prevent destructive collisions. From April, 25-29 2018, Fraunhofer FHR and its partners will exhibit the complementary radar systems TIRA and GESTRA as well as the latest radar techniques for space observation across three stands at the ILA Berlin.

The "traffic situation" in space is very tense: the Earth is currently being orbited not only by countless satellites but also by a large volume of space...

Im Focus: Researchers Discover New Anti-Cancer Protein

An international team of researchers has discovered a new anti-cancer protein. The protein, called LHPP, prevents the uncontrolled proliferation of cancer cells in the liver. The researchers led by Prof. Michael N. Hall from the Biozentrum, University of Basel, report in “Nature” that LHPP can also serve as a biomarker for the diagnosis and prognosis of liver cancer.

The incidence of liver cancer, also known as hepatocellular carcinoma, is steadily increasing. In the last twenty years, the number of cases has almost doubled...

Im Focus: Researchers at Fraunhofer monitor re-entry of Chinese space station Tiangong-1

In just a few weeks from now, the Chinese space station Tiangong-1 will re-enter the Earth's atmosphere where it will to a large extent burn up. It is possible that some debris will reach the Earth's surface. Tiangong-1 is orbiting the Earth uncontrolled at a speed of approx. 29,000 km/h.Currently the prognosis relating to the time of impact currently lies within a window of several days. The scientists at Fraunhofer FHR have already been monitoring Tiangong-1 for a number of weeks with their TIRA system, one of the most powerful space observation radars in the world, with a view to supporting the German Space Situational Awareness Center and the ESA with their re-entry forecasts.

Following the loss of radio contact with Tiangong-1 in 2016 and due to the low orbital height, it is now inevitable that the Chinese space station will...

Im Focus: Alliance „OLED Licht Forum“ – Key partner for OLED lighting solutions

Fraunhofer Institute for Organic Electronics, Electron Beam and Plasma Technology FEP, provider of research and development services for OLED lighting solutions, announces the founding of the “OLED Licht Forum” and presents latest OLED design and lighting solutions during light+building, from March 18th – 23rd, 2018 in Frankfurt a.M./Germany, at booth no. F91 in Hall 4.0.

They are united in their passion for OLED (organic light emitting diodes) lighting with all of its unique facets and application possibilities. Thus experts in...

Im Focus: Mars' oceans formed early, possibly aided by massive volcanic eruptions

Oceans formed before Tharsis and evolved together, shaping climate history of Mars

A new scenario seeking to explain how Mars' putative oceans came and went over the last 4 billion years implies that the oceans formed several hundred million...

All Focus news of the innovation-report >>>



Industry & Economy
Event News

New solar solutions for sustainable buildings and cities

23.03.2018 | Event News

Virtual reality conference comes to Reutlingen

19.03.2018 | Event News

Ultrafast Wireless and Chip Design at the DATE Conference in Dresden

16.03.2018 | Event News

Latest News

For graphite pellets, just add elbow grease

23.03.2018 | Materials Sciences

Unique communication strategy discovered in stem cell pathway controlling plant growth

23.03.2018 | Agricultural and Forestry Science

Sharpening the X-ray view of the nanocosm

23.03.2018 | Physics and Astronomy

Science & Research
Overview of more VideoLinks >>>