Forum for Science, Industry and Business

Sponsored by:     3M 
Search our Site:


Viruses on the Attack


Using a combination of imaging techniques, researchers have determined the mechanics that allow some viruses to invade cells by piercing their outer membranes and digesting their cell walls. The researchers combined their findings with earlier studies to create a near-complete scenario for that form of viral assault.

The results have a dual benefit: they show the inner workings of complex, viral nanomachines infecting cells (in a process nearly identical to some viral infections of human cells) and the images provide design tips for engineers hoping to build the gene delivery devices of the future.

The study, by researchers from Purdue University and the Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry in Moscow, appears in the August 20, 2004, issue of Cell.

Led by Michael Rossmann and Vadim Mesyanzhinov, the team added their findings to several decades of research into the structure of bacteriophage T4 – a virus that attacks the familiar pathogen Escherichia coli (E. coli). The work was supported by grants from the National Science Foundation, The Human Frontier Science Program and the Howard Hughes Medical Institute.

Although some strains of E. coli can cause food poisoning, other strains supply essential products to the human gut. It is possible that studies of viruses could one day help biologists develop strategies to fight deadly bacterial infections. Similar efforts targeting antibiotic-resistant bacteria are already underway in other laboratories.

The researchers combined x-ray crystallographic data, which gives 3-D atomic details of the constituent viral proteins, with cryo-electron microscopy images to determine how proteins in the T4 phage rearrange themselves during cell infection. Cryo-electron microscopy is similar to standard electron microscopy, except the specimens are first frozen to slow down radiation damage and hence improve the clarity of the images.

By combining thousands of images of the virus viewed from different directions, the researchers were able to determine a three dimensional structure at about 17 Ångstrom resolution, a distance spanned by just a few atoms. The end result is a model of how bacteriophage T4 infects cells.

Now that the researchers have established relationships between the component proteins, they will be analyzing the conformational changes that occur during infection. As part of their continuing work, the researchers are also looking at similar processes in other viruses to determine common essential features and differences related to the specific adaptation of each virus type.

From the researchers:

"The work opens up the door to further application of ‘hybrid’ techniques such as we used by combining crystallography and electron microscopy" – Michael Rossmann, Hanley Professor of Biological Sciences at Purdue University

"The results give hope that viruses might be targeted to find specific cells where they would then inject the cell with a genome that included useful new genes for the targeted cell." – Michael Rossmann

"The work is an excellent example of what can be achieved by a team effort, where each person plays a critical and vital role. We were extremely fortunate to have extraordinarily talented scientists such as Petr Leiman and Victor Kostyuchenko as well as equally talented participation of Paul Chipman who did all the electron microscopy data collection." – Michael Rossmann

From experts at NSF:

"This work shows, at the atomic level, how a bacteriophage can break through a bacterial cell wall. Researchers are using the bacteriophage components that specialize in dissolving as the core of a new and emerging strategy to fight bacterial pathogens, especially microbes that have developed resistance to traditional antibiotics." – Patrick Dennis, Program Director for Microbial Genetics at the National Science Foundation

"Viruses – these beautiful machines – are showing us how to develop nanotechnologies with a broad range of applications." – Parag Chitnis, Program Director for Molecular Biochemistry at the National Science Foundation

| newswise
Further information:

More articles from Life Sciences:

nachricht International team discovers novel Alzheimer's disease risk gene among Icelanders
24.10.2016 | Baylor College of Medicine

nachricht New bacteria groups, and stunning diversity, discovered underground
24.10.2016 | DOE/Lawrence Berkeley National Laboratory

All articles from Life Sciences >>>

The most recent press releases about innovation >>>

Die letzten 5 Focus-News des innovations-reports im Überblick:

Im Focus: New 3-D wiring technique brings scalable quantum computers closer to reality

Researchers from the Institute for Quantum Computing (IQC) at the University of Waterloo led the development of a new extensible wiring technique capable of controlling superconducting quantum bits, representing a significant step towards to the realization of a scalable quantum computer.

"The quantum socket is a wiring method that uses three-dimensional wires based on spring-loaded pins to address individual qubits," said Jeremy Béjanin, a PhD...

Im Focus: Scientists develop a semiconductor nanocomposite material that moves in response to light

In a paper in Scientific Reports, a research team at Worcester Polytechnic Institute describes a novel light-activated phenomenon that could become the basis for applications as diverse as microscopic robotic grippers and more efficient solar cells.

A research team at Worcester Polytechnic Institute (WPI) has developed a revolutionary, light-activated semiconductor nanocomposite material that can be used...

Im Focus: Diamonds aren't forever: Sandia, Harvard team create first quantum computer bridge

By forcefully embedding two silicon atoms in a diamond matrix, Sandia researchers have demonstrated for the first time on a single chip all the components needed to create a quantum bridge to link quantum computers together.

"People have already built small quantum computers," says Sandia researcher Ryan Camacho. "Maybe the first useful one won't be a single giant quantum computer...

Im Focus: New Products - Highlights of COMPAMED 2016

COMPAMED has become the leading international marketplace for suppliers of medical manufacturing. The trade fair, which takes place every November and is co-located to MEDICA in Dusseldorf, has been steadily growing over the past years and shows that medical technology remains a rapidly growing market.

In 2016, the joint pavilion by the IVAM Microtechnology Network, the Product Market “High-tech for Medical Devices”, will be located in Hall 8a again and will...

Im Focus: Ultra-thin ferroelectric material for next-generation electronics

'Ferroelectric' materials can switch between different states of electrical polarization in response to an external electric field. This flexibility means they show promise for many applications, for example in electronic devices and computer memory. Current ferroelectric materials are highly valued for their thermal and chemical stability and rapid electro-mechanical responses, but creating a material that is scalable down to the tiny sizes needed for technologies like silicon-based semiconductors (Si-based CMOS) has proven challenging.

Now, Hiroshi Funakubo and co-workers at the Tokyo Institute of Technology, in collaboration with researchers across Japan, have conducted experiments to...

All Focus news of the innovation-report >>>



Event News

#IC2S2: When Social Science meets Computer Science - GESIS will host the IC2S2 conference 2017

14.10.2016 | Event News

Agricultural Trade Developments and Potentials in Central Asia and the South Caucasus

14.10.2016 | Event News

World Health Summit – Day Three: A Call to Action

12.10.2016 | Event News

Latest News

New method increases energy density in lithium batteries

24.10.2016 | Power and Electrical Engineering

International team discovers novel Alzheimer's disease risk gene among Icelanders

24.10.2016 | Life Sciences

New bacteria groups, and stunning diversity, discovered underground

24.10.2016 | Life Sciences

More VideoLinks >>>