Forum for Science, Industry and Business

Sponsored by:     3M 
Search our Site:

 

UNC research accelerates discovery of novel gene function

07.07.2004


University of North Carolina at Chapel Hill researchers have developed a new technique for rapidly identifying the functions of genes.

The "high throughput" technique can be used in both cell culture and in animal models to screen thousands of genes for a particular biological function. It provides a method for the rapid development of a cDNA library, which would contain protein-encoding sequences of DNA. Researchers then can use the library to analyze a specific gene function.

The report appears in the July issue of Molecular Therapy, the American Society of Gene Therapy’s journal.



"All the genes in the human genome have now been sequenced, but the problem is that we don’t know their function," said Dr. Tal Kafri, principal investigator of the study and an assistant professor of microbiology and immunology at UNC’s School of Medicine. He also is a member of the UNC Gene Therapy Center.

The study helps to resolve two bottlenecks in determining gene functions, he said.

"It offers a quick and efficient way to transfer cDNA into a viral vector library, and it also helps isolate altered cells, ensuring that the changes in them are due to the introduced gene. The closed system we have developed allows us to take candidate genes from virus to bacteria to cell to animal, quickly and efficiently."

The technique system may have clinical applications, including drug design, Kafri added. "We could easily modify the library to find peptides or small molecules with potential to act as inhibitors for a particular cellular state or pathway. Doing so would drastically accelerate the process of high-throughput drug design and testing, taking gene candidates from cell culture to the animal model."

The technique uses a genetically engineered HIV-1 virus, in which genes can be shuttled among bacteria, cell culture or animal models, in the same vehicle, or vector. This avoids time-consuming methods involved in gene isolation and amplification, such as the polymerase chain reaction, or PCR, procedure.

The method, designed by Kafri and gene therapy center researcher Hong Ma, enables scientists to rapidly screen cells for changes in a particular phenotype. They can easily isolate and identify the gene causing the changes and place it into an animal model or bacteria for further study.

L.H. Lang | EurekAlert!
Further information:
http://www.med.unc.edu

More articles from Life Sciences:

nachricht New technology offers fast peptide synthesis
28.02.2017 | Massachusetts Institute of Technology

nachricht Biofuel produced by microalgae
28.02.2017 | Tokyo Institute of Technology

All articles from Life Sciences >>>

The most recent press releases about innovation >>>

Die letzten 5 Focus-News des innovations-reports im Überblick:

Im Focus: Safe glide at total engine failure with ELA-inside

On January 15, 2009, Chesley B. Sullenberger was celebrated world-wide: after the two engines had failed due to bird strike, he and his flight crew succeeded after a glide flight with an Airbus A320 in ditching on the Hudson River. All 155 people on board were saved.

On January 15, 2009, Chesley B. Sullenberger was celebrated world-wide: after the two engines had failed due to bird strike, he and his flight crew succeeded...

Im Focus: Breakthrough with a chain of gold atoms

In the field of nanoscience, an international team of physicists with participants from Konstanz has achieved a breakthrough in understanding heat transport

In the field of nanoscience, an international team of physicists with participants from Konstanz has achieved a breakthrough in understanding heat transport

Im Focus: DNA repair: a new letter in the cell alphabet

Results reveal how discoveries may be hidden in scientific “blind spots”

Cells need to repair damaged DNA in our genes to prevent the development of cancer and other diseases. Our cells therefore activate and send “repair-proteins”...

Im Focus: Dresdner scientists print tomorrow’s world

The Fraunhofer IWS Dresden and Technische Universität Dresden inaugurated their jointly operated Center for Additive Manufacturing Dresden (AMCD) with a festive ceremony on February 7, 2017. Scientists from various disciplines perform research on materials, additive manufacturing processes and innovative technologies, which build up components in a layer by layer process. This technology opens up new horizons for component design and combinations of functions. For example during fabrication, electrical conductors and sensors are already able to be additively manufactured into components. They provide information about stress conditions of a product during operation.

The 3D-printing technology, or additive manufacturing as it is often called, has long made the step out of scientific research laboratories into industrial...

Im Focus: Mimicking nature's cellular architectures via 3-D printing

Research offers new level of control over the structure of 3-D printed materials

Nature does amazing things with limited design materials. Grass, for example, can support its own weight, resist strong wind loads, and recover after being...

All Focus news of the innovation-report >>>

Anzeige

Anzeige

Event News

Booth and panel discussion – The Lindau Nobel Laureate Meetings at the AAAS 2017 Annual Meeting

13.02.2017 | Event News

Complex Loading versus Hidden Reserves

10.02.2017 | Event News

International Conference on Crystal Growth in Freiburg

09.02.2017 | Event News

 
Latest News

New technology offers fast peptide synthesis

28.02.2017 | Life Sciences

WSU research advances energy savings for oil, gas industries

28.02.2017 | Power and Electrical Engineering

Who can find the fish that makes the best sound?

28.02.2017 | Information Technology

VideoLinks
B2B-VideoLinks
More VideoLinks >>>