Forum for Science, Industry and Business

Sponsored by:     3M 
Search our Site:

 

Jefferson Researchers Develop Microchip to Track Genetic Signature of Cancer and Normal Tissue

22.06.2004


MicroRNAs (miRNAs), tiny pieces of genetic material that can serve as stop signs for gene expression and protein synthesis, are thought to be important in the development of cancer. Now, researchers at Jefferson Medical College and the Kimmel Cancer Center of Thomas Jefferson University in Philadelphia have developed a technique that allows them to find which miRNA genes are expressed – and how – in both cancerous and normal tissue.


Scientists, led by Carlo Croce, M.D., director of Jefferson’s Kimmel Cancer Center and professor of microbiology and immunology at Jefferson Medical College, have developed a microarray chip onto which they were able to put all the known miRNA genes in both human and mouse. They found that each tissue they tested had its own characteristic pattern of miRNA gene expression.

The work might enable scientists to gain a better understanding of the roles of miRNAs in cancer and provide targets for future drug development. They reported their findings June 21, 2004 online in the Proceedings of the National Academy of Sciences.

Dr. Croce explains that miRNAs are thought to play important roles in regulating gene expression during development and cell differentiation. MiRNAs inhibit the function of their targets, typically messenger RNA, which is involved in gene expression. They either degrade the messenger RNA or block its translation. Cells in organisms from yeast to mammals make interfering RNA to shut off gene expression in development.



Dr. Croce and his colleagues had previously shown that deletions in miRNA genes were involved in B-cell chronic lymphocytic leukemia (CLL), the most common adult leukemia in the Western world. They also had reported that human miRNA genes are frequently located in “fragile” areas of the genome that are vulnerable to mutation. “We think the miRNA genes are in fact involved in many human cancers,” he says.

“As a result, we have a number of markers that allow us to characterize specific tissue in specific cells,” Dr. Croce says. “Now the chip allows us to compare normal tissue to malignant tissue.

“This kind of approach will give us important clues about the gene regulation in a number of cells in normal and cancer tissue,” he says. “It opens new avenues for treatment because these miRNA genes are so small, they can get into cells and be used as drugs. Characterizing their targets might help in understanding cancer phenotypes.”

MiRNA genes can function differently. Take miR-16, for example, which is one of the miRNA genes Dr. Croce has studied in CLL. It functions as a tumor suppressor, and probably some of its targets are oncogenes, he says. Tumor suppressor genes are normal genes that protect against the development of cancer. Oncogenes, on the other hand, promote excessive cell growth, the hallmark of cancer.

“If the miR is expressed at a high level, however, the RNA level of the targets would be low and the expression of the oncogene would be low,” he says. Conversely, “knocking out” the miR gene would mean the expression of the oncogene would be high.

“MiRNAs are a new mechanism involved in malignant transformation,” he says. “I think it will be found to be a very generalized mechanism and provide a lot of opportunities for treatment.

“The chip is an easy way to test for miRNA alterations,” he says. “When you look at a cancer, the chip will tell you which miRNAs are in fact there and which are not. Then you can study the targets and figure out their role in cancer.

“The next step is to continue to find out how these genes are regulated and what they regulate – their targets and their roles in cancers.”

Steven Benowitz | EurekAlert!
Further information:
http://www.jefferson.edu

More articles from Life Sciences:

nachricht Water world
20.11.2017 | Washington University in St. Louis

nachricht Carefully crafted light pulses control neuron activity
20.11.2017 | University of Illinois at Urbana-Champaign

All articles from Life Sciences >>>

The most recent press releases about innovation >>>

Die letzten 5 Focus-News des innovations-reports im Überblick:

Im Focus: A “cosmic snake” reveals the structure of remote galaxies

The formation of stars in distant galaxies is still largely unexplored. For the first time, astron-omers at the University of Geneva have now been able to closely observe a star system six billion light-years away. In doing so, they are confirming earlier simulations made by the University of Zurich. One special effect is made possible by the multiple reflections of images that run through the cosmos like a snake.

Today, astronomers have a pretty accurate idea of how stars were formed in the recent cosmic past. But do these laws also apply to older galaxies? For around a...

Im Focus: Visual intelligence is not the same as IQ

Just because someone is smart and well-motivated doesn't mean he or she can learn the visual skills needed to excel at tasks like matching fingerprints, interpreting medical X-rays, keeping track of aircraft on radar displays or forensic face matching.

That is the implication of a new study which shows for the first time that there is a broad range of differences in people's visual ability and that these...

Im Focus: Novel Nano-CT device creates high-resolution 3D-X-rays of tiny velvet worm legs

Computer Tomography (CT) is a standard procedure in hospitals, but so far, the technology has not been suitable for imaging extremely small objects. In PNAS, a team from the Technical University of Munich (TUM) describes a Nano-CT device that creates three-dimensional x-ray images at resolutions up to 100 nanometers. The first test application: Together with colleagues from the University of Kassel and Helmholtz-Zentrum Geesthacht the researchers analyzed the locomotory system of a velvet worm.

During a CT analysis, the object under investigation is x-rayed and a detector measures the respective amount of radiation absorbed from various angles....

Im Focus: Researchers Develop Data Bus for Quantum Computer

The quantum world is fragile; error correction codes are needed to protect the information stored in a quantum object from the deteriorating effects of noise. Quantum physicists in Innsbruck have developed a protocol to pass quantum information between differently encoded building blocks of a future quantum computer, such as processors and memories. Scientists may use this protocol in the future to build a data bus for quantum computers. The researchers have published their work in the journal Nature Communications.

Future quantum computers will be able to solve problems where conventional computers fail today. We are still far away from any large-scale implementation,...

Im Focus: Wrinkles give heat a jolt in pillared graphene

Rice University researchers test 3-D carbon nanostructures' thermal transport abilities

Pillared graphene would transfer heat better if the theoretical material had a few asymmetric junctions that caused wrinkles, according to Rice University...

All Focus news of the innovation-report >>>

Anzeige

Anzeige

Event News

Ecology Across Borders: International conference brings together 1,500 ecologists

15.11.2017 | Event News

Road into laboratory: Users discuss biaxial fatigue-testing for car and truck wheel

15.11.2017 | Event News

#Berlin5GWeek: The right network for Industry 4.0

30.10.2017 | Event News

 
Latest News

Water world

20.11.2017 | Life Sciences

Less is more to produce top-notch 2D materials

20.11.2017 | Materials Sciences

Carefully crafted light pulses control neuron activity

20.11.2017 | Life Sciences

VideoLinks
B2B-VideoLinks
More VideoLinks >>>