Forum for Science, Industry and Business

Sponsored by:     3M 
Search our Site:

 

Common Worm Provides Insights Into Salmonella Virulence

08.06.2004


Using a common worm as a model, researchers from Duke University Medical Center have identified specific genes within Salmonella that give the bacteria its ability to infect host cells.


Alejandro Aballay, Ph.D.
CREDIT: Duke University Medical Center



They said their findings could ultimately lead to improved drugs to prevent or treat Salmonella infections.

The researchers found four genes related to the Salmonella’s "molecular syringe" that are required for the bacteria to have maximum potency in infecting the worm, known as Caenorhabditis elegans. The virulence factors -- injected into the host cells by Salmonella -- act by blunting the innate immune system of C. elegans from attacking and destroying the infecting bacteria.


These findings are important, the researchers said, because the C. elegans innate immune response to Salmonella infection is quite similar to that of mammals, including humans. For this reason, the researchers said that their C elegans model can provide new genetic insights into the relationships between invading microbes and their target host cells.

The results of the Duke experiments were published June 8, 2004, in the journal Current Biology. The research was supported by the National Institutes of Health and the Whitehead Institute for Biomedical Research.

"C elegans lives in the soil, so it continually comes into contact with bacteria and other microbes," said lead researcher microbiologist Alejandro Aballay, Ph.D. "It has a highly developed system for not only recognizing bacteria, but also responding to them. The ability of its innate immune system to respond appropriately to specific bacteria is very similar to that of mammals.

"The goal of our research is not only to better understand what makes a particular pathogen harmful for the host, but to determine those characteristics of the host that either make it resistant or susceptible to the pathogen," Aballay added. "To be successful in this type of research, one cannot just focus on the pathogen; the relationship between the pathogen and host is so important."

C elegans is an ideal model for genetic studies, Aballay said, because the worm takes only three days to develop from an embryo to an adult capable of reproducing. Also, scientists can easily manipulate specific genes in the worm, and as opposed to other animal models, large quantities of the animals can be grown quickly. They can even be frozen and used at a later date, Aballay said.

Additionally, the worm is essentially a long intestinal tract, which is also advantageous as a model for Salmonella virulence, since a primary target of Salmonella infections in mammals is the intestine. Aballay said this may be the reason of the remarkable overlap between Salmonella virulence factors required for pathogenesis in mammals and worms.

For their experiments, the team used the C. elegans-Salmonella enterica model developed by Aballay. Specifically, the researchers examined one of the bacteria’s five known "pathogenecity islands," or clusters of genes that have accumulated in S. enterica bacteria through time. These genes have been acquired by the bacteria through the course of evolution as a way of adapting and improving its chances of successfully infecting its hosts, Aballay explained.

"In this study we have related several genes located in pathogenicity islands to S. enterica pathogenesis in worms," Aballay said.

Within the pathogencity island 1 is the "molecular syringe," known as the Type III secretion system (TTSS). This TTSS punctures the cell wall of the host and injects the virulence factors inside. One of the specific virulence factors injected into the host cells through the TTSS, termed SptP, was critical for the Salmonella’s ability to kill C. elegans.

"While there are at least 13 effector proteins, or virulence factors, which are injected into the host through the TTSS, our studies have shown that a single protein (SptP) can have a major impact on an entire animal," Aballay explained. "What this factor does is diminish the animal’s ability to mount an effective immune response."

Aballay believes that this model opens news areas of research for better understanding the relationship between pathogens and host responses.

"While many virulence factors have been identified, the mechanisms by which they contribute to bacterial pathogenesis remain unknown," he said. "But with the identification of the targeted pathways in the host, we should be able to figure out how virulence factors influence the innate immune response."

Aballay said that the C elegans system could be an effective living model for testing or screening new compounds or drugs for treating Salmonella in humans. However, he added, much additional research in other living models is necessary before these new insights can be applied to human disease.

Using the C elegans model, Aballay and his colleagues are now conducting genetic screens to find genetic variants called polymorphisms in the host that appear to confer either resistance or susceptibility to Salmonella. The researchers expect that these polymorphisms would also play a role in human disease.

Other members of the research team were Duke’s Jennifer Tenor, as well as Beth McCormick, Ph.D., and Frederick Ausubel, Ph.D., of Harvard Medical School, Boston.

Richard Merritt | dukemed news
Further information:
http://dukemednews.org/news/article.php?id=7647

More articles from Life Sciences:

nachricht Could this protein protect people against coronary artery disease?
17.11.2017 | University of North Carolina Health Care

nachricht Microbial resident enables beetles to feed on a leafy diet
17.11.2017 | Max-Planck-Institut für chemische Ökologie

All articles from Life Sciences >>>

The most recent press releases about innovation >>>

Die letzten 5 Focus-News des innovations-reports im Überblick:

Im Focus: A “cosmic snake” reveals the structure of remote galaxies

The formation of stars in distant galaxies is still largely unexplored. For the first time, astron-omers at the University of Geneva have now been able to closely observe a star system six billion light-years away. In doing so, they are confirming earlier simulations made by the University of Zurich. One special effect is made possible by the multiple reflections of images that run through the cosmos like a snake.

Today, astronomers have a pretty accurate idea of how stars were formed in the recent cosmic past. But do these laws also apply to older galaxies? For around a...

Im Focus: Visual intelligence is not the same as IQ

Just because someone is smart and well-motivated doesn't mean he or she can learn the visual skills needed to excel at tasks like matching fingerprints, interpreting medical X-rays, keeping track of aircraft on radar displays or forensic face matching.

That is the implication of a new study which shows for the first time that there is a broad range of differences in people's visual ability and that these...

Im Focus: Novel Nano-CT device creates high-resolution 3D-X-rays of tiny velvet worm legs

Computer Tomography (CT) is a standard procedure in hospitals, but so far, the technology has not been suitable for imaging extremely small objects. In PNAS, a team from the Technical University of Munich (TUM) describes a Nano-CT device that creates three-dimensional x-ray images at resolutions up to 100 nanometers. The first test application: Together with colleagues from the University of Kassel and Helmholtz-Zentrum Geesthacht the researchers analyzed the locomotory system of a velvet worm.

During a CT analysis, the object under investigation is x-rayed and a detector measures the respective amount of radiation absorbed from various angles....

Im Focus: Researchers Develop Data Bus for Quantum Computer

The quantum world is fragile; error correction codes are needed to protect the information stored in a quantum object from the deteriorating effects of noise. Quantum physicists in Innsbruck have developed a protocol to pass quantum information between differently encoded building blocks of a future quantum computer, such as processors and memories. Scientists may use this protocol in the future to build a data bus for quantum computers. The researchers have published their work in the journal Nature Communications.

Future quantum computers will be able to solve problems where conventional computers fail today. We are still far away from any large-scale implementation,...

Im Focus: Wrinkles give heat a jolt in pillared graphene

Rice University researchers test 3-D carbon nanostructures' thermal transport abilities

Pillared graphene would transfer heat better if the theoretical material had a few asymmetric junctions that caused wrinkles, according to Rice University...

All Focus news of the innovation-report >>>

Anzeige

Anzeige

Event News

Ecology Across Borders: International conference brings together 1,500 ecologists

15.11.2017 | Event News

Road into laboratory: Users discuss biaxial fatigue-testing for car and truck wheel

15.11.2017 | Event News

#Berlin5GWeek: The right network for Industry 4.0

30.10.2017 | Event News

 
Latest News

NASA detects solar flare pulses at Sun and Earth

17.11.2017 | Physics and Astronomy

NIST scientists discover how to switch liver cancer cell growth from 2-D to 3-D structures

17.11.2017 | Health and Medicine

The importance of biodiversity in forests could increase due to climate change

17.11.2017 | Studies and Analyses

VideoLinks
B2B-VideoLinks
More VideoLinks >>>