New storage method amplifies cells available for science
Like many other kinds of cells used in biomedical research, human embryonic stem cells are stored and transported in a cryopreserved state, frozen to -320 degrees Fahrenheit, the temperature of their liquid nitrogen storage bath.
But when scientists thaw the cells for use in the lab, less than 1 percent awake from their frigid slumber and assume their undifferentiated state. This blank slate form is characteristic of stem cells and essential for the basic science required before the promising cells are ready for the clinic. So scientists are required to place the few survivors in culture and painstakingly tend to them for weeks before new colonies are abundant enough to conduct experiments.
Sean P. Palecek | EurekAlert!
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