A cell responds to pollutants - such as dioxin - via intricate and complex biochemical pathways beginning with the interaction of the pollutant molecule with a cell surface receptor. Christopher Bradfield and colleagues used yeast as a model system to elucidate the steps involved in the pathway that regulates vertebrate cell response to dioxin, the aryl hydrocarbon receptor (AHR) signal transduction pathway. To assess the molecules and pathways involved in the AHR pathway, the research group studied 4507 yeast "deletion" strains, each strain missing one gene from its genome. In this way Bradfield and colleagues identified 54 genes that had a significant influence on AHR response. Only two of these genes, termed modifiers, had been previously identified.
Though yeast does not naturally possess AHR, it is an ideal genetic model due to its small, well-characterized genome and similarity to vertebrate systems. Because yeast have been so well studied, the researchers were able to construct a "protein interaction network," (PIN) based on previously known interactions between the proteins encoded by the 54 modifier genes. The resulting map revealed groups of highly connected, related modifiers. The authors show that these modifiers group into five discrete biochemical steps in the pathway of dioxin signaling. Not only did they succeed in identifying potentially new genes involved in the signaling pathway but they were able to identify one of the modules as a previously undescribed nuclear step in the signaling pathway.
Citation: Yao G, Craven M, Drinkwater N, Bradfield CA (2004) Interaction Networks in Yeast Define and Enumerate the Signaling Steps of the Vertebrate Aryl Hydrocarbon Receptor. PLoS Biol: e65 DOI: 10.1371/journal.pbio.0020065
Christopher Bradfield | EurekAlert!
Rainbow colors reveal cell history: Uncovering β-cell heterogeneity
22.09.2017 | DFG-Forschungszentrum für Regenerative Therapien TU Dresden
The pyrenoid is a carbon-fixing liquid droplet
22.09.2017 | Max-Planck-Institut für Biochemie
A warming planet
19.09.2017 | Event News
12.09.2017 | Event News
06.09.2017 | Event News
22.09.2017 | Life Sciences
22.09.2017 | Medical Engineering
22.09.2017 | Physics and Astronomy