Forum for Science, Industry and Business

Sponsored by:     3M 
Search our Site:

 

Reprogrammable Cells From Fat Are True Adult Stem Cells

09.03.2004


After successfully turning cells taken from human fat into different cell types, Duke University Medical Center researchers have now demonstrated that these specific cells are truly adult stem cells with multiple potential, instead of being a mixture of different types of cells, each with a more limited destiny.


Farshid Guilak, Ph.D



During the past three years, the Duke researchers exposed cells taken from human liposuction procedures to different cocktails of nutrients and vitamins, and "reprogrammed" them to grown into bone, cartilage, fat and nerve cells. At the time, they termed these cells adipose-derived stromal cells.

However, as a result of the latest set of experiments, the researchers are now confident that the majority of these cells are indeed truly adult stem cells that have the potential to be reprogrammed into traveling down multiple developmental paths. This is important, they said, because these cells could be a single, readily available source for creating new cells and tissues to treat disease.


The results of the Duke study were presented March 8, 2004, at the 50th annual scientific meeting of the Orthopedic Research Society by Kristen Lott, a fourth-year medical student working in the laboratory of Farshid Guilak, Ph.D., director of orthopedic research and senior member of the Duke team.

"Our findings indicate that 62 percent of the human fat cells could be reprogrammed into turning into at least two other different cell types," Lott said. "This percentage of cells is quite high, meaning that they have a great deal of flexibility and that their ultimate destiny may not be so pre-determined.

"These results suggest that these cells are truly stem cells that could provide a source of undifferentiated cells for multiples uses," Lott continued. "We’re still a long way from using these cells as therapies in humans, but we’re excited about the progress we’ve made so far."

Added Guilak, who is also on the faculty of Duke’s Pratt School of Engineering, "We don’t know exactly why body fat contains stem cells that can form bone or cartilage, but it does help dispel the dogma that adult stem cells can only be found in the bone marrow."

For their study, the Duke team took liposuction samples from three adult donors and then grew clones of these cells for up to 25 doublings. The cells were then exposed in culture to one of the four recipes -- mixtures of vitamins, growth factors and steroids -- for reprogramming cells into either fat, bone, cartilage or nerve cells.

While 62 percent of the cells were able to be "guided" down at least two different paths, only 10 percent failed to differentiate into any of the four cell types, Lott said.

"Additionally, the results of this study offer criteria for defining stem cell multipotency that should help researchers in further investigations," Guilak said. "More of the clones developed into bone, cartilage and nerve cells than they did into fat cells, which is another interesting finding."

Guilak believes that as a result of the successive culturing, the stem cells may have lost their ability to turn into fat cells.

"Our experiments took the cells through many doublings," Lott said. "Since these cells would potentially be in people for longer, we still need to better understand what happens to these cells over time."

The researchers anticipate that the first patients to benefit from this research are those who have suffered some sort of cartilage damage due to injury or trauma. Farther down the line, they foresee a time when entire joints ravaged by osteoarthritis can be relined with bioengineered cartilage.

"We don’t currently have a satisfactory remedy for people who suffer a cartilage-damaging injury," Guilak said. "There is a real need for a new approach to treating these injuries. We envision being able to remove a little bit of fat, and then grow customized, three-dimensional pieces of cartilage that would then be surgically implanted in the joint. One of the beauties of this system is that since the cells are from the same patients, there are no worries of adverse immune responses or disease transmission."

The study was funded by the National Institutes of Health and the North Carolina Biotechnology Center.

Other members of the research team were Hani Awad, Ph.D., from Duke and Jeffrey Gimble, M.D., from the Pennington Biomedical Research Center at Louisiana State University.

Richard Merritt | dukemed news
Further information:
http://dukemednews.org/news/article.php?id=7452

More articles from Life Sciences:

nachricht New catalyst controls activation of a carbon-hydrogen bond
21.11.2017 | Emory Health Sciences

nachricht The main switch
21.11.2017 | Albert-Ludwigs-Universität Freiburg im Breisgau

All articles from Life Sciences >>>

The most recent press releases about innovation >>>

Die letzten 5 Focus-News des innovations-reports im Überblick:

Im Focus: Nanoparticles help with malaria diagnosis – new rapid test in development

The WHO reports an estimated 429,000 malaria deaths each year. The disease mostly affects tropical and subtropical regions and in particular the African continent. The Fraunhofer Institute for Silicate Research ISC teamed up with the Fraunhofer Institute for Molecular Biology and Applied Ecology IME and the Institute of Tropical Medicine at the University of Tübingen for a new test method to detect malaria parasites in blood. The idea of the research project “NanoFRET” is to develop a highly sensitive and reliable rapid diagnostic test so that patient treatment can begin as early as possible.

Malaria is caused by parasites transmitted by mosquito bite. The most dangerous form of malaria is malaria tropica. Left untreated, it is fatal in most cases....

Im Focus: A “cosmic snake” reveals the structure of remote galaxies

The formation of stars in distant galaxies is still largely unexplored. For the first time, astron-omers at the University of Geneva have now been able to closely observe a star system six billion light-years away. In doing so, they are confirming earlier simulations made by the University of Zurich. One special effect is made possible by the multiple reflections of images that run through the cosmos like a snake.

Today, astronomers have a pretty accurate idea of how stars were formed in the recent cosmic past. But do these laws also apply to older galaxies? For around a...

Im Focus: Visual intelligence is not the same as IQ

Just because someone is smart and well-motivated doesn't mean he or she can learn the visual skills needed to excel at tasks like matching fingerprints, interpreting medical X-rays, keeping track of aircraft on radar displays or forensic face matching.

That is the implication of a new study which shows for the first time that there is a broad range of differences in people's visual ability and that these...

Im Focus: Novel Nano-CT device creates high-resolution 3D-X-rays of tiny velvet worm legs

Computer Tomography (CT) is a standard procedure in hospitals, but so far, the technology has not been suitable for imaging extremely small objects. In PNAS, a team from the Technical University of Munich (TUM) describes a Nano-CT device that creates three-dimensional x-ray images at resolutions up to 100 nanometers. The first test application: Together with colleagues from the University of Kassel and Helmholtz-Zentrum Geesthacht the researchers analyzed the locomotory system of a velvet worm.

During a CT analysis, the object under investigation is x-rayed and a detector measures the respective amount of radiation absorbed from various angles....

Im Focus: Researchers Develop Data Bus for Quantum Computer

The quantum world is fragile; error correction codes are needed to protect the information stored in a quantum object from the deteriorating effects of noise. Quantum physicists in Innsbruck have developed a protocol to pass quantum information between differently encoded building blocks of a future quantum computer, such as processors and memories. Scientists may use this protocol in the future to build a data bus for quantum computers. The researchers have published their work in the journal Nature Communications.

Future quantum computers will be able to solve problems where conventional computers fail today. We are still far away from any large-scale implementation,...

All Focus news of the innovation-report >>>

Anzeige

Anzeige

Event News

Ecology Across Borders: International conference brings together 1,500 ecologists

15.11.2017 | Event News

Road into laboratory: Users discuss biaxial fatigue-testing for car and truck wheel

15.11.2017 | Event News

#Berlin5GWeek: The right network for Industry 4.0

30.10.2017 | Event News

 
Latest News

Previous evidence of water on mars now identified as grainflows

21.11.2017 | Physics and Astronomy

NASA's James Webb Space Telescope completes final cryogenic testing

21.11.2017 | Physics and Astronomy

New catalyst controls activation of a carbon-hydrogen bond

21.11.2017 | Life Sciences

VideoLinks
B2B-VideoLinks
More VideoLinks >>>