Forum for Science, Industry and Business

Sponsored by:     3M 
Search our Site:

 

Researchers reveal early steps in clone development

26.11.2003


Despite widely publicized reports about the sheep, Dolly and Polly, cloning is still not considered successful in the scientific community. Only two percent of clones succeed and they are sometimes unhealthy. To understand exactly where cloning goes wrong, researchers at Temple University School of Medicine (TUSM) examined and compared the earliest stages of development in normal embryos and cloned embryos.



"First, we mapped out some of the early steps an egg and sperm take to become an embryo," said Keith Latham, PhD, associate professor of biochemistry at TUSM and lead author of the study. "Next, we examined how well clones were able to replicate these early steps. We discovered that at this stage of development, 100 percent of clones replicated the process entirely. This tells us that the problems must occur later in the development process."

The study, "Rapid H1 linker histone transitions following fertilization or somatic cell nuclear transfer: evidence for a uniform developmental program in mice," will appear in an upcoming issue of Developmental Biology. The study is part of a larger program, directed by Latham and funded by two National Institutes of Health (NIH) grants, that is examining how eggs communicate with chromosomes.


"When a sperm and egg unite, each brings a set of chromosomes to the table. Molecules in the egg turn the two sets of chromosomes, known as genomes, into an embryo. During cloning, we ask the egg to do the same thing but with different starting materials," said Latham. "Instead of a sperm, the egg has to work with an adult cell from the organism that is being cloned. We used to think that during cloning, the egg integrated the adult cell as easily as it does the sperm.

"However, once the first few steps of development occur, the rest of the process is actually quite slow and incomplete. Cloned embryos bear characteristics of both an embryo and an adult cell. They’re not very happy and healthy."

Latham suspects part of the problem is the culture used to house the cells in the laboratory. "We have cultures that work very well for embryos and cultures that work very well for adult cells. However, we still need to find the optimal culture media for cloned embryos. Once we find out what that is, cloning will probably be more successful," said Latham.

"Understanding the early development process could help us increase success rates for cloning and its potential applications, such as producing valuable farm animals and preserving endangered species," said Latham.

"As remarkable as it is to see clones born, cloning is really just a simple but striking demonstration of the truly remarkable processes that are at the root of each new life. We take this for granted, because it happens so readily, and yet when one gains an appreciation for the many complex things that must occur in order for each of us to be in the world, it really sinks in just how terrific the process is," exclaimed Latham.

Temple researchers collaborated with researchers at the University of Utah Health Sciences Center and Peregrine pharmaceuticals on the project.

Eryn Jelesiewicz | EurekAlert!
Further information:
http://www.temple.edu/

More articles from Life Sciences:

nachricht Single-stranded DNA and RNA origami go live
15.12.2017 | Wyss Institute for Biologically Inspired Engineering at Harvard

nachricht New antbird species discovered in Peru by LSU ornithologists
15.12.2017 | Louisiana State University

All articles from Life Sciences >>>

The most recent press releases about innovation >>>

Die letzten 5 Focus-News des innovations-reports im Überblick:

Im Focus: First-of-its-kind chemical oscillator offers new level of molecular control

DNA molecules that follow specific instructions could offer more precise molecular control of synthetic chemical systems, a discovery that opens the door for engineers to create molecular machines with new and complex behaviors.

Researchers have created chemical amplifiers and a chemical oscillator using a systematic method that has the potential to embed sophisticated circuit...

Im Focus: Long-lived storage of a photonic qubit for worldwide teleportation

MPQ scientists achieve long storage times for photonic quantum bits which break the lower bound for direct teleportation in a global quantum network.

Concerning the development of quantum memories for the realization of global quantum networks, scientists of the Quantum Dynamics Division led by Professor...

Im Focus: Electromagnetic water cloak eliminates drag and wake

Detailed calculations show water cloaks are feasible with today's technology

Researchers have developed a water cloaking concept based on electromagnetic forces that could eliminate an object's wake, greatly reducing its drag while...

Im Focus: Scientists channel graphene to understand filtration and ion transport into cells

Tiny pores at a cell's entryway act as miniature bouncers, letting in some electrically charged atoms--ions--but blocking others. Operating as exquisitely sensitive filters, these "ion channels" play a critical role in biological functions such as muscle contraction and the firing of brain cells.

To rapidly transport the right ions through the cell membrane, the tiny channels rely on a complex interplay between the ions and surrounding molecules,...

Im Focus: Towards data storage at the single molecule level

The miniaturization of the current technology of storage media is hindered by fundamental limits of quantum mechanics. A new approach consists in using so-called spin-crossover molecules as the smallest possible storage unit. Similar to normal hard drives, these special molecules can save information via their magnetic state. A research team from Kiel University has now managed to successfully place a new class of spin-crossover molecules onto a surface and to improve the molecule’s storage capacity. The storage density of conventional hard drives could therefore theoretically be increased by more than one hundred fold. The study has been published in the scientific journal Nano Letters.

Over the past few years, the building blocks of storage media have gotten ever smaller. But further miniaturization of the current technology is hindered by...

All Focus news of the innovation-report >>>

Anzeige

Anzeige

Event News

See, understand and experience the work of the future

11.12.2017 | Event News

Innovative strategies to tackle parasitic worms

08.12.2017 | Event News

AKL’18: The opportunities and challenges of digitalization in the laser industry

07.12.2017 | Event News

 
Latest News

Engineers program tiny robots to move, think like insects

15.12.2017 | Power and Electrical Engineering

One in 5 materials chemistry papers may be wrong, study suggests

15.12.2017 | Materials Sciences

New antbird species discovered in Peru by LSU ornithologists

15.12.2017 | Life Sciences

VideoLinks
B2B-VideoLinks
More VideoLinks >>>