Forum for Science, Industry and Business

Sponsored by:     3M 
Search our Site:

 

Dartmouth bioengineers develop humanized yeast

29.08.2003


Bioengineers at Dartmouth have genetically engineered yeast to produce humanized therapeutic proteins to address the manufacturing crunch currently confronting the biopharmaceutical industry. Reported in this week’s issue of Science, the researchers have re-engineered the yeast P. pastoris to secrete a complex human glycoprotein--a process offering significant advantages over current production methods using mammalian cell lines, according to the researchers.

The study, titled "Production of Complex Human Glycoproteins in Yeast," is one result of a collaboration between researchers at Dartmouth’s Thayer School of Engineering and GlycoFi, Inc., a biotech company located in Lebanon, New Hampshire. Founded in the spring of 2000 by Dartmouth engineering professors Tillman Gerngross and Charles Hutchinson, GlycoFi is advancing technology for the production of humanized proteins using fungal-based expression systems.

"For the first time, we have shown that yeast can be used to produce a complex human glycoprotein," says Professor Gerngross. "This technology has the potential to revolutionize the way therapeutic proteins are made--better, cheaper, faster, safer--and offer a level of control over the quality of the end product that has never existed before."



Proteins for pharmaceuticals must be manufactured by living cells. These cells are genetically engineered to produce (or express) proteins that mimic the ones synthesized by humans. These proteins can then be used to treat diseases ranging from cancer and multiple sclerosis to hemophelia and renal disease.

Current production of these therapeutic proteins, however, is limited by capacity due to rapid growth in the discovery of protein-based therapies--to the point that some approved drugs cannot be produced in adequate amounts, and still others are not making it into commercialization due to cost-prohibitive production methods.

"This development is very timely considering the production capacity bottleneck that’s facing today’s biomanufacturing industry," notes Hutchinson, CEO of GlycoFi and Dean Emeritus of Thayer School.

The Dartmouth researchers genetically engineered the yeast P. pastoris to perform a series of sequential reactions (pathways) that mimic the processing of proteins in humans. After eliminating non-human pathways from the yeast, five genes were inserted causing the yeast to construct a new secretion pathway that synthesizes human-like glycoprotein structures of superior quality.

"The protein structures we are seeing in our yeast are of a purity and uniformity unprecedented in biopharmaceutical manufacturing," said Stefan Wildt, Director of Strain Devlopment at GlycoFi, and one of the authors of the paper. "This makes it possible to harness both the inherent advantages of fungal protein expression systems and the potential to significantly increase pharmaceutical production capacities, therefore ultimately improving patient access to life-saving drug therapies."

Sue Knapp | EurekAlert!
Further information:
http://www.dartmouth.edu/

More articles from Life Sciences:

nachricht Multi-institutional collaboration uncovers how molecular machines assemble
02.12.2016 | Salk Institute

nachricht Fertilized egg cells trigger and monitor loss of sperm’s epigenetic memory
02.12.2016 | IMBA - Institut für Molekulare Biotechnologie der Österreichischen Akademie der Wissenschaften GmbH

All articles from Life Sciences >>>

The most recent press releases about innovation >>>

Die letzten 5 Focus-News des innovations-reports im Überblick:

Im Focus: Novel silicon etching technique crafts 3-D gradient refractive index micro-optics

A multi-institutional research collaboration has created a novel approach for fabricating three-dimensional micro-optics through the shape-defined formation of porous silicon (PSi), with broad impacts in integrated optoelectronics, imaging, and photovoltaics.

Working with colleagues at Stanford and The Dow Chemical Company, researchers at the University of Illinois at Urbana-Champaign fabricated 3-D birefringent...

Im Focus: Quantum Particles Form Droplets

In experiments with magnetic atoms conducted at extremely low temperatures, scientists have demonstrated a unique phase of matter: The atoms form a new type of quantum liquid or quantum droplet state. These so called quantum droplets may preserve their form in absence of external confinement because of quantum effects. The joint team of experimental physicists from Innsbruck and theoretical physicists from Hannover report on their findings in the journal Physical Review X.

“Our Quantum droplets are in the gas phase but they still drop like a rock,” explains experimental physicist Francesca Ferlaino when talking about the...

Im Focus: MADMAX: Max Planck Institute for Physics takes up axion research

The Max Planck Institute for Physics (MPP) is opening up a new research field. A workshop from November 21 - 22, 2016 will mark the start of activities for an innovative axion experiment. Axions are still only purely hypothetical particles. Their detection could solve two fundamental problems in particle physics: What dark matter consists of and why it has not yet been possible to directly observe a CP violation for the strong interaction.

The “MADMAX” project is the MPP’s commitment to axion research. Axions are so far only a theoretical prediction and are difficult to detect: on the one hand,...

Im Focus: Molecules change shape when wet

Broadband rotational spectroscopy unravels structural reshaping of isolated molecules in the gas phase to accommodate water

In two recent publications in the Journal of Chemical Physics and in the Journal of Physical Chemistry Letters, researchers around Melanie Schnell from the Max...

Im Focus: Fraunhofer ISE Develops Highly Compact, High Frequency DC/DC Converter for Aviation

The efficiency of power electronic systems is not solely dependent on electrical efficiency but also on weight, for example, in mobile systems. When the weight of relevant components and devices in airplanes, for instance, is reduced, fuel savings can be achieved and correspondingly greenhouse gas emissions decreased. New materials and components based on gallium nitride (GaN) can help to reduce weight and increase the efficiency. With these new materials, power electronic switches can be operated at higher switching frequency, resulting in higher power density and lower material costs.

Researchers at the Fraunhofer Institute for Solar Energy Systems ISE together with partners have investigated how these materials can be used to make power...

All Focus news of the innovation-report >>>

Anzeige

Anzeige

Event News

ICTM Conference 2017: Production technology for turbomachine manufacturing of the future

16.11.2016 | Event News

Innovation Day Laser Technology – Laser Additive Manufacturing

01.11.2016 | Event News

#IC2S2: When Social Science meets Computer Science - GESIS will host the IC2S2 conference 2017

14.10.2016 | Event News

 
Latest News

UTSA study describes new minimally invasive device to treat cancer and other illnesses

02.12.2016 | Medical Engineering

Plasma-zapping process could yield trans fat-free soybean oil product

02.12.2016 | Agricultural and Forestry Science

What do Netflix, Google and planetary systems have in common?

02.12.2016 | Physics and Astronomy

VideoLinks
B2B-VideoLinks
More VideoLinks >>>