Forum for Science, Industry and Business

Sponsored by:     3M 
Search our Site:

 

A New Twist on DNA

17.07.2003


Using a tool kit of lasers, tiny beads and a Lego set, Howard Hughes Medical Institute researchers have made the first measurement of the torsional, or twisting, elasticity of a single molecule of DNA.



The measurements reveal that DNA is significantly stiffer than previously thought and, when wound, may in fact provide enough power to be used as a sort of molecular, rubberband motor to propel nanomachines. Although that type of application may be well in the future, the studies are significant because they offer a blueprint for measuring the contortions that DNA undergoes during replication and other key processes.

The researchers, led by Howard Hughes Medical Institute investigator Carlos Bustamante, reported their research in the July 17, 2003, issue of the journal Nature. Bustamante and the paper’s two lead authors, graduate students Zev Bryant and Michael Stone, are at the University of California, Berkeley.


“This finding is important because many of the processes involved in reading the information in DNA involve distorting the DNA molecule,” said Bustamante. “And to truly understand these processes, we need to understand the energy costs involved in the interaction between the protein that induces distortion and the DNA.”

Almost ten years ago, Bustamante and his colleagues measured the extensional elasticity of single-strand DNA, by attaching a DNA molecule at either end to tiny beads. Using a laser “magnetic tweezers” instrument, the researchers applied a precisely known force to stretch the molecule. However, measuring the torsional stiffness of the molecule proved far more difficult.

“For almost seven years, I couldn’t convince any graduate student or postdoc to do these experiments,” said Bustamante. “I would tell them my idea, which I thought was really great, and they would look at me, smile and say, `Yeah, yeah, great, nice idea … next?’ Finally, Zev and Mike came to my office and said, `We’re going to try this crazy experiment of yours. It’s not going to work, but we’ll try it, anyway.’”

Bustamante’s scheme involved attaching the ends of a single DNA molecule between two tiny beads as they had done before. However, in the torque-measurement experiments, the researchers then biochemically “nicked” a point on the double-strand DNA to create a single chemical bond swivel. Near this nick, on the side of the rotating bead, the researchers attached a third “indicator” bead. They then rapidly wound the DNA molecule up thousands of times — while holding the rotor bead steady with flow — using a twisting robot built from Legos. After the winding process, they stopped the flow and followed the unwinding of the DNA molecule by looking at the spinning of the rotor bead in real time.

By measuring the resulting spins of a series of indicator beads of differing diameters as the molecule untwisted, the researchers obtained data that they could analyze to measure the DNA molecule’s torsional stiffness.

Bustamante and his colleagues discovered from this analysis that the DNA molecule was about 40 percent more resistant to twisting than had been reported by other researchers. “We’re very surprised and excited about this finding because it represents the first direct measurement of the torsional stiffness of a single DNA molecule,” said Bustamante. “The other measurements were done on molecules in bulk, and were indirect.”

According to Bustamante, the conclusive measurement of torsional elasticity will enable researchers to understand better the “partitioning” of mechanical energy as a DNA molecule undergoes twisting during biological processes. While some fraction of the energy twists the double-stranded spiral of the molecule itself, another fraction creates “writhing” — a hairpin looping of the molecule like an over-twisted rope. “This value of forty percent more tells us we may need to revise our ideas about such partitioning,” said Bustamante.

Importantly, he said, the ability to measure molecular torque will enable a new class of experiments to study the mechanical behavior of protein enzymes that interact with the DNA molecule. For example, Bustamante and his colleagues are now using the same experimental apparatus to explore how the enzyme DNA polymerase — which copies a single DNA strand by pulling itself along the strand’s length — exerts torque on the DNA strand as it works.

More speculative, said Bustamante, is the idea that the DNA molecule might provide energy to power molecule-sized nanomotors. “We found that if you pull the DNA molecule, it will overstretch and must unwind. During this process, the mechanical linear force applied to the end of the molecule gets transformed into the generation of torque. If you relax the molecule, it will rewind, generating torque in the opposite direction. The molecule then behaves as a reversible force-torque converter,” said Bustamante. “If you attached rotational elements to the molecule, when you pulled the molecule, it would begin rotating and would drive the molecule as a motor.”

Contact: Jim Keeley, keeleyj@hhmi.org

Jim Keeley | Howard Hughes Medical Institute
Further information:
http://www.hhmi.org

More articles from Life Sciences:

nachricht Making fuel out of thick air
08.12.2017 | DOE/Argonne National Laboratory

nachricht ‘Spying’ on the hidden geometry of complex networks through machine intelligence
08.12.2017 | Technische Universität Dresden

All articles from Life Sciences >>>

The most recent press releases about innovation >>>

Die letzten 5 Focus-News des innovations-reports im Überblick:

Im Focus: Scientists channel graphene to understand filtration and ion transport into cells

Tiny pores at a cell's entryway act as miniature bouncers, letting in some electrically charged atoms--ions--but blocking others. Operating as exquisitely sensitive filters, these "ion channels" play a critical role in biological functions such as muscle contraction and the firing of brain cells.

To rapidly transport the right ions through the cell membrane, the tiny channels rely on a complex interplay between the ions and surrounding molecules,...

Im Focus: Towards data storage at the single molecule level

The miniaturization of the current technology of storage media is hindered by fundamental limits of quantum mechanics. A new approach consists in using so-called spin-crossover molecules as the smallest possible storage unit. Similar to normal hard drives, these special molecules can save information via their magnetic state. A research team from Kiel University has now managed to successfully place a new class of spin-crossover molecules onto a surface and to improve the molecule’s storage capacity. The storage density of conventional hard drives could therefore theoretically be increased by more than one hundred fold. The study has been published in the scientific journal Nano Letters.

Over the past few years, the building blocks of storage media have gotten ever smaller. But further miniaturization of the current technology is hindered by...

Im Focus: Successful Mechanical Testing of Nanowires

With innovative experiments, researchers at the Helmholtz-Zentrums Geesthacht and the Technical University Hamburg unravel why tiny metallic structures are extremely strong

Light-weight and simultaneously strong – porous metallic nanomaterials promise interesting applications as, for instance, for future aeroplanes with enhanced...

Im Focus: Virtual Reality for Bacteria

An interdisciplinary group of researchers interfaced individual bacteria with a computer to build a hybrid bio-digital circuit - Study published in Nature Communications

Scientists at the Institute of Science and Technology Austria (IST Austria) have managed to control the behavior of individual bacteria by connecting them to a...

Im Focus: A space-time sensor for light-matter interactions

Physicists in the Laboratory for Attosecond Physics (run jointly by LMU Munich and the Max Planck Institute for Quantum Optics) have developed an attosecond electron microscope that allows them to visualize the dispersion of light in time and space, and observe the motions of electrons in atoms.

The most basic of all physical interactions in nature is that between light and matter. This interaction takes place in attosecond times (i.e. billionths of a...

All Focus news of the innovation-report >>>

Anzeige

Anzeige

Event News

See, understand and experience the work of the future

11.12.2017 | Event News

Innovative strategies to tackle parasitic worms

08.12.2017 | Event News

AKL’18: The opportunities and challenges of digitalization in the laser industry

07.12.2017 | Event News

 
Latest News

Midwife and signpost for photons

11.12.2017 | Physics and Astronomy

How do megacities impact coastal seas? Searching for evidence in Chinese marginal seas

11.12.2017 | Earth Sciences

PhoxTroT: Optical Interconnect Technologies Revolutionized Data Centers and HPC Systems

11.12.2017 | Information Technology

VideoLinks
B2B-VideoLinks
More VideoLinks >>>