Madrid, Spain: Scientists believe that they are an important step nearer to success in creating an artificial egg from the combination of the nucleus of a somatic cell and an oocyte which has had its DNA-carrying nucleus removed, a conference of international fertility experts heard today (Tuesday 1 July).
Dr Peter Nagy, from Reproductive Biology Associates, Atlanta, collaborating with the University of Connecticut, USA, told the European Society of Human Reproduction and Embryology annual conference that former approaches to haploidisation using a fully mature oocyte and a resting (interphase) somatic cell had caused misaligned chromosomes during cell division. However, he was confident from his teams latest experiments that this difficulty could be overcome, even though their new approach also ran into some problems.
"We decided to initiate haploidisation at an earlier stage in the oocytes cell cycle, when it was still immature, but this time using a somatic cell in its active (metaphase or G2/M) stage. Essentially, we took the control of the first nuclear division away from the oocyte and gave it to the somatic cell," he said.
Margaret Willson | EurekAlert!
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