Forum for Science, Industry and Business

Sponsored by:     3M 
Search our Site:

 

Chemists create unusual lariat RNA, a key intermediate in biological splicing

19.05.2003


The production of lariat RNAs is a key step in the biologically important process of splicing. Because splicing changes the protein that is made from a given gene, a fundamental understanding of splicing is critical for comprehending the connections between genes and proteins. The study of splicing, however, has been very difficult in part because lariat RNAs have been nearly impossible to make artificially.


Chemical reaction catalyzed by a DNA enzyme that creates lariat RNA.


Stylized diagram of a lariat RNA showing the branch site. The various colors represent the different nucleotides A, G, C and U.



Now, chemistry professor Scott K. Silverman and graduate student Yangming Wang at the University of Illinois at Urbana-Champaign have found artificial deoxyribozymes (DNA enzymes) that synthesize branched and lariat RNAs. The researchers report their discovery in a paper that has been accepted for publication in the Journal of the American Chemical Society, and posted on its Web site

"Lariat RNAs are crucial intermediates in the biological splicing of messenger RNAs," Silverman said. "The lariat RNAs synthesized by our new DNA enzymes should help provide a detailed biochemical understanding of RNA splicing."


Lariat RNAs have an unusual connectivity in which a key "branch site" nucleotide is the meeting point between three RNA strands, two of which connect to form a loop, Silverman said. "The resulting molecule, which resembles a lariat, has the same connectivity as introns (intervening regions that don’t perform coding functions) that are removed from messenger RNAs during biological splicing in fungi, eukaryotes, and even some bacteria."

Silverman and Wang used a process called in vitro selection to sample billions of DNA sequences for the desired chemical selectivity. The DNA enzymes the researchers found can catalyze branched RNA formation at rates up to 5 million times faster than the reaction would occur in the absence of the enzyme.

"The ability to make these branches and lariats offers us a new path for the biochemical investigation of RNA splicing," Silverman said. "Errors in making proteins have been linked to certain diseases and birth defects, for example. By incorporating chemical modifications into these key structural intermediates, we can more thoroughly examine the fundamental splicing process."


###
The March of Dimes, National Institutes of Health, Petroleum Research Fund and the University of Illinois funded the work

James E. Kloeppel | EurekAlert!
Further information:
http://www.uiuc.edu/

More articles from Life Sciences:

nachricht Chains of nanogold – forged with atomic precision
23.09.2016 | Suomen Akatemia (Academy of Finland)

nachricht Self-assembled nanostructures hit their target
23.09.2016 | King Abdullah University of Science and Technology

All articles from Life Sciences >>>

The most recent press releases about innovation >>>

Die letzten 5 Focus-News des innovations-reports im Überblick:

Im Focus: OLED microdisplays in data glasses for improved human-machine interaction

The Fraunhofer Institute for Organic Electronics, Electron Beam and Plasma Technology FEP has been developing various applications for OLED microdisplays based on organic semiconductors. By integrating the capabilities of an image sensor directly into the microdisplay, eye movements can be recorded by the smart glasses and utilized for guidance and control functions, as one example. The new design will be debuted at Augmented World Expo Europe (AWE) in Berlin at Booth B25, October 18th – 19th.

“Augmented-reality” and “wearables” have become terms we encounter almost daily. Both can make daily life a little simpler and provide valuable assistance for...

Im Focus: Artificial Intelligence Helps in the Discovery of New Materials

With the help of artificial intelligence, chemists from the University of Basel in Switzerland have computed the characteristics of about two million crystals made up of four chemical elements. The researchers were able to identify 90 previously unknown thermodynamically stable crystals that can be regarded as new materials. They report on their findings in the scientific journal Physical Review Letters.

Elpasolite is a glassy, transparent, shiny and soft mineral with a cubic crystal structure. First discovered in El Paso County (Colorado, USA), it can also be...

Im Focus: Complex hardmetal tools out of the 3D printer

For the first time, Fraunhofer IKTS shows additively manufactured hardmetal tools at WorldPM 2016 in Hamburg. Mechanical, chemical as well as a high heat resistance and extreme hardness are required from tools that are used in mechanical and automotive engineering or in plastics and building materials industry. Researchers at the Fraunhofer Institute for Ceramic Technologies and Systems IKTS in Dresden managed the production of complex hardmetal tools via 3D printing in a quality that are in no way inferior to conventionally produced high-performance tools.

Fraunhofer IKTS counts decades of proven expertise in the development of hardmetals. To date, reliable cutting, drilling, pressing and stamping tools made of...

Im Focus: Launch of New Industry Working Group for Process Control in Laser Material Processing

At AKL’16, the International Laser Technology Congress held in May this year, interest in the topic of process control was greater than expected. Appropriately, the event was also used to launch the Industry Working Group for Process Control in Laser Material Processing. The group provides a forum for representatives from industry and research to initiate pre-competitive projects and discuss issues such as standards, potential cost savings and feasibility.

In the age of industry 4.0, laser technology is firmly established within manufacturing. A wide variety of laser techniques – from USP ablation and additive...

Im Focus: New laser joining technologies at ‘K 2016’ trade fair

Every three years, the plastics industry gathers at K, the international trade fair for plastics and rubber in Düsseldorf. The Fraunhofer Institute for Laser Technology ILT will also be attending again and presenting many innovative technologies, such as for joining plastics and metals using ultrashort pulse lasers. From October 19 to 26, you can find the Fraunhofer ILT at the joint Fraunhofer booth SC01 in Hall 7.

K is the world’s largest trade fair for the plastics and rubber industry. As in previous years, the organizers are expecting 3,000 exhibitors and more than...

All Focus news of the innovation-report >>>

Anzeige

Anzeige

Event News

Experts from industry and academia discuss the future mobile telecommunications standard 5G

23.09.2016 | Event News

ICPE in Graz for the seventh time

20.09.2016 | Event News

Using mathematical models to understand our brain

16.09.2016 | Event News

 
Latest News

Chains of nanogold – forged with atomic precision

23.09.2016 | Life Sciences

New leukemia treatment offers hope

23.09.2016 | Health and Medicine

Self-assembled nanostructures hit their target

23.09.2016 | Life Sciences

VideoLinks
B2B-VideoLinks
More VideoLinks >>>