Forum for Science, Industry and Business

Sponsored by:     3M 
Search our Site:

 

Scientists Develop Transgenic Chicken to Study Embryo Development

11.03.2003


North Carolina State University poultry scientists have developed a powerful new tool to aid the understanding of how chicken embryos develop.

The research of Dr. Paul Mozdziak, assistant professor of poultry science, and Dr. James Petitte, professor of poultry science, resulted in successfully transferring a gene into a chicken and establishing a line of chickens carrying that specific marker gene.

Currently, the chick embryo is often used as a model to understand normal and abnormal embryo development. The new lines of transgenic chicken provide a new tool that can be employed in studies aimed at understanding birth defects such as limb deformities and spina bifida. The researchers say learning the mechanisms behind how cells behave during embryo development could eventually provide clues to halting developmental disabilities and may lead to other uses not yet imagined, including improvements in human and animal health.



The research appears in the March edition of Developmental Dynamics.

“Although there are people who have made transgenic chickens before, no one has produced a transgenic chicken expressing a reporter gene that can be easily tracked,” Mozdziak says. “We can now take cells from our transgenic chicken and put those cells into a chick embryo or another transgenic chicken and see how the cells behave and interact with each other.”

“This tool provides the impetus to go to the next level of looking at avian stem cells in embryos, putting these stem cells in different places and seeing where they end up,” Petitte says.
The researchers say gene transfer is much more complicated in chickens than in, say, mice. Chicken embryos contain about 50,000 cells before the egg is laid; gene transfer in other mammals involves inserting DNA into just one cell.

Mozdziak and Petitte developed the transgenic chicken by taking a RNA virus, or retrovirus, carrying a reporter gene – the lacZ gene, which is easy to detect and which expresses a protein, beta-galactosidase – and injecting it into the blastoderm, or layer of cells on the surface of the yolk, of freshly laid chicken eggs. The eggs were allowed to hatch, and chickens were screened for the presence of the lacZ gene. Eight of 15 male chickens that lived to sexual maturity carried the lacZ gene in their semen, the researchers say.

These eight chickens were then mated with female non-transgenic chickens. Of the chicks produced, two males tested positive for the lacZ gene. These two males were mated with normal females and 50 percent of their offspring contained the lacZ gene as expected.

Further, the second-generation chickens expressed beta-galactosidase, and the lacZ gene is apparently stable from generation to generation.

Petitte says that other transgenic chickens have carried the lacZ gene, but that this is the first time that a transgenic chicken line that expresses beta-galactosidase has been developed.

“This is a really powerful research tool, and it’s the first time anyone has had this tool in avian biology,” he said.

_petitte@ncsu.edu | North Carolina State University
Further information:
http://www.ncsu.edu/news/press_releases/03_03/71.htm

More articles from Life Sciences:

nachricht Oestrogen regulates pathological changes of bones via bone lining cells
28.07.2017 | Veterinärmedizinische Universität Wien

nachricht Programming cells with computer-like logic
27.07.2017 | Wyss Institute for Biologically Inspired Engineering at Harvard

All articles from Life Sciences >>>

The most recent press releases about innovation >>>

Die letzten 5 Focus-News des innovations-reports im Überblick:

Im Focus: Abrupt motion sharpens x-ray pulses

Spectrally narrow x-ray pulses may be “sharpened” by purely mechanical means. This sounds surprisingly, but a team of theoretical and experimental physicists developed and realized such a method. It is based on fast motions, precisely synchronized with the pulses, of a target interacting with the x-ray light. Thereby, photons are redistributed within the x-ray pulse to the desired spectral region.

A team of theoretical physicists from the MPI for Nuclear Physics (MPIK) in Heidelberg has developed a novel method to intensify the spectrally broad x-ray...

Im Focus: Physicists Design Ultrafocused Pulses

Physicists working with researcher Oriol Romero-Isart devised a new simple scheme to theoretically generate arbitrarily short and focused electromagnetic fields. This new tool could be used for precise sensing and in microscopy.

Microwaves, heat radiation, light and X-radiation are examples for electromagnetic waves. Many applications require to focus the electromagnetic fields to...

Im Focus: Carbon Nanotubes Turn Electrical Current into Light-emitting Quasi-particles

Strong light-matter coupling in these semiconducting tubes may hold the key to electrically pumped lasers

Light-matter quasi-particles can be generated electrically in semiconducting carbon nanotubes. Material scientists and physicists from Heidelberg University...

Im Focus: Flexible proximity sensor creates smart surfaces

Fraunhofer IPA has developed a proximity sensor made from silicone and carbon nanotubes (CNT) which detects objects and determines their position. The materials and printing process used mean that the sensor is extremely flexible, economical and can be used for large surfaces. Industry and research partners can use and further develop this innovation straight away.

At first glance, the proximity sensor appears to be nothing special: a thin, elastic layer of silicone onto which black square surfaces are printed, but these...

Im Focus: 3-D scanning with water

3-D shape acquisition using water displacement as the shape sensor for the reconstruction of complex objects

A global team of computer scientists and engineers have developed an innovative technique that more completely reconstructs challenging 3D objects. An ancient...

All Focus news of the innovation-report >>>

Anzeige

Anzeige

Event News

Clash of Realities 2017: Registration now open. International Conference at TH Köln

26.07.2017 | Event News

Closing the Sustainability Circle: Protection of Food with Biobased Materials

21.07.2017 | Event News

»We are bringing Additive Manufacturing to SMEs«

19.07.2017 | Event News

 
Latest News

New 3-D imaging reveals how human cell nucleus organizes DNA and chromatin of its genome

28.07.2017 | Health and Medicine

Heavy metals in water meet their match

28.07.2017 | Power and Electrical Engineering

Oestrogen regulates pathological changes of bones via bone lining cells

28.07.2017 | Life Sciences

VideoLinks
B2B-VideoLinks
More VideoLinks >>>