Forum for Science, Industry and Business

Sponsored by:     3M 
Search our Site:

 

Scientists Develop Transgenic Chicken to Study Embryo Development

11.03.2003


North Carolina State University poultry scientists have developed a powerful new tool to aid the understanding of how chicken embryos develop.

The research of Dr. Paul Mozdziak, assistant professor of poultry science, and Dr. James Petitte, professor of poultry science, resulted in successfully transferring a gene into a chicken and establishing a line of chickens carrying that specific marker gene.

Currently, the chick embryo is often used as a model to understand normal and abnormal embryo development. The new lines of transgenic chicken provide a new tool that can be employed in studies aimed at understanding birth defects such as limb deformities and spina bifida. The researchers say learning the mechanisms behind how cells behave during embryo development could eventually provide clues to halting developmental disabilities and may lead to other uses not yet imagined, including improvements in human and animal health.



The research appears in the March edition of Developmental Dynamics.

“Although there are people who have made transgenic chickens before, no one has produced a transgenic chicken expressing a reporter gene that can be easily tracked,” Mozdziak says. “We can now take cells from our transgenic chicken and put those cells into a chick embryo or another transgenic chicken and see how the cells behave and interact with each other.”

“This tool provides the impetus to go to the next level of looking at avian stem cells in embryos, putting these stem cells in different places and seeing where they end up,” Petitte says.
The researchers say gene transfer is much more complicated in chickens than in, say, mice. Chicken embryos contain about 50,000 cells before the egg is laid; gene transfer in other mammals involves inserting DNA into just one cell.

Mozdziak and Petitte developed the transgenic chicken by taking a RNA virus, or retrovirus, carrying a reporter gene – the lacZ gene, which is easy to detect and which expresses a protein, beta-galactosidase – and injecting it into the blastoderm, or layer of cells on the surface of the yolk, of freshly laid chicken eggs. The eggs were allowed to hatch, and chickens were screened for the presence of the lacZ gene. Eight of 15 male chickens that lived to sexual maturity carried the lacZ gene in their semen, the researchers say.

These eight chickens were then mated with female non-transgenic chickens. Of the chicks produced, two males tested positive for the lacZ gene. These two males were mated with normal females and 50 percent of their offspring contained the lacZ gene as expected.

Further, the second-generation chickens expressed beta-galactosidase, and the lacZ gene is apparently stable from generation to generation.

Petitte says that other transgenic chickens have carried the lacZ gene, but that this is the first time that a transgenic chicken line that expresses beta-galactosidase has been developed.

“This is a really powerful research tool, and it’s the first time anyone has had this tool in avian biology,” he said.

_petitte@ncsu.edu | North Carolina State University
Further information:
http://www.ncsu.edu/news/press_releases/03_03/71.htm

More articles from Life Sciences:

nachricht A Map of the Cell’s Power Station
18.08.2017 | Albert-Ludwigs-Universität Freiburg im Breisgau

nachricht On the way to developing a new active ingredient against chronic infections
18.08.2017 | Deutsches Zentrum für Infektionsforschung

All articles from Life Sciences >>>

The most recent press releases about innovation >>>

Die letzten 5 Focus-News des innovations-reports im Überblick:

Im Focus: Fizzy soda water could be key to clean manufacture of flat wonder material: Graphene

Whether you call it effervescent, fizzy, or sparkling, carbonated water is making a comeback as a beverage. Aside from quenching thirst, researchers at the University of Illinois at Urbana-Champaign have discovered a new use for these "bubbly" concoctions that will have major impact on the manufacturer of the world's thinnest, flattest, and one most useful materials -- graphene.

As graphene's popularity grows as an advanced "wonder" material, the speed and quality at which it can be manufactured will be paramount. With that in mind,...

Im Focus: Exotic quantum states made from light: Physicists create optical “wells” for a super-photon

Physicists at the University of Bonn have managed to create optical hollows and more complex patterns into which the light of a Bose-Einstein condensate flows. The creation of such highly low-loss structures for light is a prerequisite for complex light circuits, such as for quantum information processing for a new generation of computers. The researchers are now presenting their results in the journal Nature Photonics.

Light particles (photons) occur as tiny, indivisible portions. Many thousands of these light portions can be merged to form a single super-photon if they are...

Im Focus: Circular RNA linked to brain function

For the first time, scientists have shown that circular RNA is linked to brain function. When a RNA molecule called Cdr1as was deleted from the genome of mice, the animals had problems filtering out unnecessary information – like patients suffering from neuropsychiatric disorders.

While hundreds of circular RNAs (circRNAs) are abundant in mammalian brains, one big question has remained unanswered: What are they actually good for? In the...

Im Focus: RAVAN CubeSat measures Earth's outgoing energy

An experimental small satellite has successfully collected and delivered data on a key measurement for predicting changes in Earth's climate.

The Radiometer Assessment using Vertically Aligned Nanotubes (RAVAN) CubeSat was launched into low-Earth orbit on Nov. 11, 2016, in order to test new...

Im Focus: Scientists shine new light on the “other high temperature superconductor”

A study led by scientists of the Max Planck Institute for the Structure and Dynamics of Matter (MPSD) at the Center for Free-Electron Laser Science in Hamburg presents evidence of the coexistence of superconductivity and “charge-density-waves” in compounds of the poorly-studied family of bismuthates. This observation opens up new perspectives for a deeper understanding of the phenomenon of high-temperature superconductivity, a topic which is at the core of condensed matter research since more than 30 years. The paper by Nicoletti et al has been published in the PNAS.

Since the beginning of the 20th century, superconductivity had been observed in some metals at temperatures only a few degrees above the absolute zero (minus...

All Focus news of the innovation-report >>>

Anzeige

Anzeige

Event News

Call for Papers – ICNFT 2018, 5th International Conference on New Forming Technology

16.08.2017 | Event News

Sustainability is the business model of tomorrow

04.08.2017 | Event News

Clash of Realities 2017: Registration now open. International Conference at TH Köln

26.07.2017 | Event News

 
Latest News

A Map of the Cell’s Power Station

18.08.2017 | Life Sciences

Engineering team images tiny quasicrystals as they form

18.08.2017 | Physics and Astronomy

Researchers printed graphene-like materials with inkjet

18.08.2017 | Materials Sciences

VideoLinks
B2B-VideoLinks
More VideoLinks >>>