Forum for Science, Industry and Business

Sponsored by:     3M 
Search our Site:

 

Physical map of mouse genome now available

05.08.2002


A physical map of the genetic makeup of a mouse - the mouse genome - is 98 percent complete and is being released online by the journal Nature. Researchers at the Genome Sequencing Center at Washington University School of Medicine in St. Louis played a major role in the international effort, as they did in the sequencing and mapping of the human genome.

"The mouse plays a vital role in research on human biology and disease," says John D. McPherson, Ph.D., associate professor of genetics and the lead investigator on the St. Louis team. "This physical map gives us the big picture of the mouse genome. It will be tremendously helpful to medical investigators and to those studying the human genome."

Comparison of the mouse and human maps, for example, can highlight regions of DNA that control genes. These regions are crucial to understanding the role of genes in health and disease, but they are difficult to find using current methods.



The physical mouse-genome map is a complementary effort to the draft sequence of the mouse genome, which was released last May. The important difference is one of detail and organization, says McPherson.

The draft sequence is a description of the chemical bases--represented by A, C, G, and T--that make up the genome. The physical map organizes and delineates this information on the mouse’s 20 chromosomes. McPherson compared the draft sequence to loose pages from an encyclopedia. The pages may provide a lot of information, but they lack context.

"Each page may provide many details," he says, "like the population and climate of a country. But until all the pages are assembled correctly, you may not know that you are reading about Zaire." A physical map places all the "pages" of DNA sequence in their correct order within each volume, with each volume being a chromosome.

Furthermore, the DNA-sequence information used to compile the physical map was gathered differently from the information used to compile the draft sequence. Because the physical map comes from a separate source of genetic information, the researchers are using it to confirm the accuracy of the draft sequence.

"We are comparing the two independent data sets to be certain they are giving us the same answer," says McPherson.

The physical map benefits medical researchers in another way, as well. It was assembled using longer segments of DNA than those used to assemble the draft sequence. The long segments were grown, or cloned, in bacteria. Now that the mapping is complete, the bacteria containing these bits of mouse genome continue to be grown, stored in freezers, and carefully cataloged. Investigators studying mouse genes or regions of DNA now can locate the location of that particular segment on the map and obtain the actual clone of that region to study, rather than isolating the region themselves.


###
The following centers contributed to the project:
The Wellcome Trust Sanger Institute, Hinxton, Cambridge, England (http://www.sanger.ac.uk)
Genome Sequencing Center, Washington University School of Medicine, St Louis (http://genome.wustl.edu/)
Genome Sciences Centre, British Columbia Cancer Agency, Vancouver, BC, Canada (http://www.bcgsc.bc.ca/)
The Institute for Genome Research, Rockville, MD (http://www.tigr.org/)
Children’s Hospital Oakland Research Institute, Oakland, CA (http://www.childrenshospitaloakland.org/)
EMBL--European Bioinformatics Institute, Hinxton, Cambridge, UK (http://www.ebi.ac.uk)
Department of Electrical Engineering, Washington University, St Louis (http://www.ee.washington.edu/)


The Genome Sequencing Center (GSC) at Washington University School of Medicine in St. Louis focuses on the large scale generation and analysis of DNA sequence. Founded in 1993, the GSC is one of the top sequencing centers in the United States.

Funding from the Wellcome Trust and the National Institutes of Health supported this research.


Darrell Ward | EurekAlert!
Further information:
http://www.nature.com
http://dx.doi.org/10.1038/nature00957

More articles from Life Sciences:

nachricht What the world's tiniest 'monster truck' reveals
23.08.2017 | American Chemical Society

nachricht Treating arthritis with algae
23.08.2017 | Empa - Eidgenössische Materialprüfungs- und Forschungsanstalt

All articles from Life Sciences >>>

The most recent press releases about innovation >>>

Die letzten 5 Focus-News des innovations-reports im Überblick:

Im Focus: Fizzy soda water could be key to clean manufacture of flat wonder material: Graphene

Whether you call it effervescent, fizzy, or sparkling, carbonated water is making a comeback as a beverage. Aside from quenching thirst, researchers at the University of Illinois at Urbana-Champaign have discovered a new use for these "bubbly" concoctions that will have major impact on the manufacturer of the world's thinnest, flattest, and one most useful materials -- graphene.

As graphene's popularity grows as an advanced "wonder" material, the speed and quality at which it can be manufactured will be paramount. With that in mind,...

Im Focus: Exotic quantum states made from light: Physicists create optical “wells” for a super-photon

Physicists at the University of Bonn have managed to create optical hollows and more complex patterns into which the light of a Bose-Einstein condensate flows. The creation of such highly low-loss structures for light is a prerequisite for complex light circuits, such as for quantum information processing for a new generation of computers. The researchers are now presenting their results in the journal Nature Photonics.

Light particles (photons) occur as tiny, indivisible portions. Many thousands of these light portions can be merged to form a single super-photon if they are...

Im Focus: Circular RNA linked to brain function

For the first time, scientists have shown that circular RNA is linked to brain function. When a RNA molecule called Cdr1as was deleted from the genome of mice, the animals had problems filtering out unnecessary information – like patients suffering from neuropsychiatric disorders.

While hundreds of circular RNAs (circRNAs) are abundant in mammalian brains, one big question has remained unanswered: What are they actually good for? In the...

Im Focus: RAVAN CubeSat measures Earth's outgoing energy

An experimental small satellite has successfully collected and delivered data on a key measurement for predicting changes in Earth's climate.

The Radiometer Assessment using Vertically Aligned Nanotubes (RAVAN) CubeSat was launched into low-Earth orbit on Nov. 11, 2016, in order to test new...

Im Focus: Scientists shine new light on the “other high temperature superconductor”

A study led by scientists of the Max Planck Institute for the Structure and Dynamics of Matter (MPSD) at the Center for Free-Electron Laser Science in Hamburg presents evidence of the coexistence of superconductivity and “charge-density-waves” in compounds of the poorly-studied family of bismuthates. This observation opens up new perspectives for a deeper understanding of the phenomenon of high-temperature superconductivity, a topic which is at the core of condensed matter research since more than 30 years. The paper by Nicoletti et al has been published in the PNAS.

Since the beginning of the 20th century, superconductivity had been observed in some metals at temperatures only a few degrees above the absolute zero (minus...

All Focus news of the innovation-report >>>

Anzeige

Anzeige

Event News

Call for Papers – ICNFT 2018, 5th International Conference on New Forming Technology

16.08.2017 | Event News

Sustainability is the business model of tomorrow

04.08.2017 | Event News

Clash of Realities 2017: Registration now open. International Conference at TH Köln

26.07.2017 | Event News

 
Latest News

What the world's tiniest 'monster truck' reveals

23.08.2017 | Life Sciences

Treating arthritis with algae

23.08.2017 | Life Sciences

Witnessing turbulent motion in the atmosphere of a distant star

23.08.2017 | Physics and Astronomy

VideoLinks
B2B-VideoLinks
More VideoLinks >>>