Forum for Science, Industry and Business

Sponsored by:     3M 
Search our Site:

 

Capturing DNA molecules in a nanochannel

25.06.2008
Precision control of DNA separation using an electric field

An electric voltage can be used to propel DNA molecules through a channel a few nanometers deep, or to stop them in their tracks.

In a strong electric field the molecules judder along the channel, while in weaker fields they move more smoothly. This enables DNA fragments to be ‘captured’ on a chip and separated for analysis. University of Twente researchers will soon publish details of this work in Nano Letters.

The researchers found that, when forced through extremely shallow channels just 20 nanometers deep and a few micrometers wide, DNA molecules behave very differently than they do in free solution. In the latter situation they tend to form clumps, while molecules in the channels are forced into an elongated straitjacket.

... more about:
»DNA »Electric »Mobility

This effect alone produces a difference in mobility between long and short molecules. Moreover, exposure to an electric field has now been shown to have a substantial effect. This presents a range of new options for the separation of fragments (and entire molecules) of DNA. The previous technique, known as gel electrophoresis, involved the use of micro-channels filled with a gel.

According to researcher Georgette Salieb-Beugelaar, the laborious and time-consuming process of pouring in the gel can be rendered obsolete by the new method.

Roughness
In their Nano Letters article, the researchers ascribe the difference in mobility to factors such as the roughness of the channels’ surfaces. A DNA molecule can easily be 1000 times longer than the channels are deep. As a result, it encounters minute surface irregularities at many different points, an effect that is reinforced by the electric field. This seems to be the cause of the stagnation in mobility that occurs in strong fields. It presents an opportunity to capture fragments and – using weaker fields - to accurately control their onward motion. This is the first demonstration of varying mobility in electric fields of differing strengths.

Wiebe van der Veen | alfa
Further information:
http://www.utwente.nl
http://www.utwente.nl/nieuws/pers/en/cont_08-030_en.doc/

Further reports about: DNA Electric Mobility

More articles from Life Sciences:

nachricht ADP-ribosylation on the right track
26.04.2018 | Max-Planck-Institut für Biologie des Alterns

nachricht Flavins keep a handy helper in their pocket
25.04.2018 | University of Freiburg

All articles from Life Sciences >>>

The most recent press releases about innovation >>>

Die letzten 5 Focus-News des innovations-reports im Überblick:

Im Focus: Why we need erasable MRI scans

New technology could allow an MRI contrast agent to 'blink off,' helping doctors diagnose disease

Magnetic resonance imaging, or MRI, is a widely used medical tool for taking pictures of the insides of our body. One way to make MRI scans easier to read is...

Im Focus: BAM@Hannover Messe: innovative 3D printing method for space flight

At the Hannover Messe 2018, the Bundesanstalt für Materialforschung und-prüfung (BAM) will show how, in the future, astronauts could produce their own tools or spare parts in zero gravity using 3D printing. This will reduce, weight and transport costs for space missions. Visitors can experience the innovative additive manufacturing process live at the fair.

Powder-based additive manufacturing in zero gravity is the name of the project in which a component is produced by applying metallic powder layers and then...

Im Focus: Molecules Brilliantly Illuminated

Physicists at the Laboratory for Attosecond Physics, which is jointly run by Ludwig-Maximilians-Universität and the Max Planck Institute of Quantum Optics, have developed a high-power laser system that generates ultrashort pulses of light covering a large share of the mid-infrared spectrum. The researchers envisage a wide range of applications for the technology – in the early diagnosis of cancer, for instance.

Molecules are the building blocks of life. Like all other organisms, we are made of them. They control our biorhythm, and they can also reflect our state of...

Im Focus: Spider silk key to new bone-fixing composite

University of Connecticut researchers have created a biodegradable composite made of silk fibers that can be used to repair broken load-bearing bones without the complications sometimes presented by other materials.

Repairing major load-bearing bones such as those in the leg can be a long and uncomfortable process.

Im Focus: Writing and deleting magnets with lasers

Study published in the journal ACS Applied Materials & Interfaces is the outcome of an international effort that included teams from Dresden and Berlin in Germany, and the US.

Scientists at the Helmholtz-Zentrum Dresden-Rossendorf (HZDR) together with colleagues from the Helmholtz-Zentrum Berlin (HZB) and the University of Virginia...

All Focus news of the innovation-report >>>

Anzeige

Anzeige

VideoLinks
Industry & Economy
Event News

Invitation to the upcoming "Current Topics in Bioinformatics: Big Data in Genomics and Medicine"

13.04.2018 | Event News

Unique scope of UV LED technologies and applications presented in Berlin: ICULTA-2018

12.04.2018 | Event News

IWOLIA: A conference bringing together German Industrie 4.0 and French Industrie du Futur

09.04.2018 | Event News

 
Latest News

Why we need erasable MRI scans

26.04.2018 | Medical Engineering

Balancing nuclear and renewable energy

26.04.2018 | Power and Electrical Engineering

Researchers 3-D print electronics and cells directly on skin

26.04.2018 | Information Technology

VideoLinks
Science & Research
Overview of more VideoLinks >>>