Forum for Science, Industry and Business

Sponsored by:     3M 
Search our Site:

 

Brucella abortus S19 genome sequenced; points toward virulence genes

09.06.2008
Researchers at the Virginia Bioinformatics Institute at Virginia Tech and the National Animal Disease Center in Ames, Iowa, and collaborators at 454 Life Sciences of Branford, Conn., have sequenced the genome of Brucella abortus strain S19.

Strain S19 is a naturally occurring strain of B. abortus that does not cause disease and was discovered by Dr. John Buck in 1923. It has been used for more than six decades as vaccine that protects cattle against brucellosis, an infectious disease caused by other strains of B. abortus that leads to reproductive failure in livestock.

Scientists have long wanted to know what genetic features make strain S19 suitable for use as a vaccine in cattle because it may hold the secret as to why other Brucella strains cause disease and trigger the abortion of developing embryos in livestock. The researchers have discovered a group of 24 genes that are linked to virulence by making comparisons of the newly available S19 genome sequence to previously sequenced genomes of two virulent strains of B. abortus. The paper "Genome sequence of Brucella abortus vaccine strain S19 compared to virulent strains yields candidate virulence genes" was published recently in PLoS One (May 2008, Volume 3, Issue 5, e2193).

Oswald Crasta, project director at VBI's Cyberinfrastructure Group and the corresponding author on the paper, remarked: "We have been able to leverage rapid sequencing of the S19 genome on the Roche GS-20™ and GS-FLX™ platforms, and comparative genomics narrowed down the search for Brucella virulence factors to a small group of genes. Of particular interest are four genes that show consistently large sequence differences in S19 compared with two fully sequenced virulent strains." He added: "Further studies are underway to characterize the short list of protein differences that appear to be involved in cellular processes ranging from lipid transport and metabolism to transcription and protein transport. We believe that this characterization will explain why strain S19 has been such a successful vaccine over the years and why infection with other strains leads to disease."

... more about:
»Brucella »GS-FLX™ »Genome »Roche »S19 »VBI »Vaccine »abortus »genes »sequenced »virulence

The initial sequencing was performed on the Roche GS-20™ at 454 Life Sciences, and subsequently repeated after the installation of the Roche GS-FLX™ in the VBI Core Laboratory Facility. Additional traditional sequencing methods were used in VBI's Core Laboratory Facility to completely finish the S19 genome sequence. Clive Evans, Associate Director of the Core Laboratory Facility at VBI, remarked: "The S19 sequence was the first bacterial genome sequenced at VBI with the new Roche GS-FLX™, which was installed in January 2007. The Roche GS-FLX™ sequence allowed us to verify and improve the original sequence, and reduced the number of gaps that needed to be covered with traditional sequencing methods. We were very pleased with the performance of the Roche GS-FLX™."

Bruno Sobral, Executive and Scientific Director of VBI and co-author, remarked: "The complete genome sequence of strain S19 helps us understand the causal molecular agents of brucellosis." He remarked: "Brucella is able to pass from animals to humans with relative ease and poses a significant public health burden for workers in the livestock industry. It is also a possible agent for agricultural, civilian and military bioterrorism. This emphasizes the need for researchers to have a handle on the genetic makeup of the different Brucella strains."

Barry Whyte | EurekAlert!
Further information:
http://www.vbi.vt.edu

Further reports about: Brucella GS-FLX™ Genome Roche S19 VBI Vaccine abortus genes sequenced virulence

More articles from Life Sciences:

nachricht One step closer to reality
20.04.2018 | Max-Planck-Institut für Entwicklungsbiologie

nachricht The dark side of cichlid fish: from cannibal to caregiver
20.04.2018 | Veterinärmedizinische Universität Wien

All articles from Life Sciences >>>

The most recent press releases about innovation >>>

Die letzten 5 Focus-News des innovations-reports im Überblick:

Im Focus: Spider silk key to new bone-fixing composite

University of Connecticut researchers have created a biodegradable composite made of silk fibers that can be used to repair broken load-bearing bones without the complications sometimes presented by other materials.

Repairing major load-bearing bones such as those in the leg can be a long and uncomfortable process.

Im Focus: Writing and deleting magnets with lasers

Study published in the journal ACS Applied Materials & Interfaces is the outcome of an international effort that included teams from Dresden and Berlin in Germany, and the US.

Scientists at the Helmholtz-Zentrum Dresden-Rossendorf (HZDR) together with colleagues from the Helmholtz-Zentrum Berlin (HZB) and the University of Virginia...

Im Focus: Gamma-ray flashes from plasma filaments

Novel highly efficient and brilliant gamma-ray source: Based on model calculations, physicists of the Max PIanck Institute for Nuclear Physics in Heidelberg propose a novel method for an efficient high-brilliance gamma-ray source. A giant collimated gamma-ray pulse is generated from the interaction of a dense ultra-relativistic electron beam with a thin solid conductor. Energetic gamma-rays are copiously produced as the electron beam splits into filaments while propagating across the conductor. The resulting gamma-ray energy and flux enable novel experiments in nuclear and fundamental physics.

The typical wavelength of light interacting with an object of the microcosm scales with the size of this object. For atoms, this ranges from visible light to...

Im Focus: Basel researchers succeed in cultivating cartilage from stem cells

Stable joint cartilage can be produced from adult stem cells originating from bone marrow. This is made possible by inducing specific molecular processes occurring during embryonic cartilage formation, as researchers from the University and University Hospital of Basel report in the scientific journal PNAS.

Certain mesenchymal stem/stromal cells from the bone marrow of adults are considered extremely promising for skeletal tissue regeneration. These adult stem...

Im Focus: Like a wedge in a hinge

Researchers lay groundwork to tailor drugs for new targets in cancer therapy

In the fight against cancer, scientists are developing new drugs to hit tumor cells at so far unused weak points. Such a “sore spot” is the protein complex...

All Focus news of the innovation-report >>>

Anzeige

Anzeige

VideoLinks
Industry & Economy
Event News

Invitation to the upcoming "Current Topics in Bioinformatics: Big Data in Genomics and Medicine"

13.04.2018 | Event News

Unique scope of UV LED technologies and applications presented in Berlin: ICULTA-2018

12.04.2018 | Event News

IWOLIA: A conference bringing together German Industrie 4.0 and French Industrie du Futur

09.04.2018 | Event News

 
Latest News

Magnetic nano-imaging on a table top

20.04.2018 | Physics and Astronomy

Start of work for the world's largest electric truck

20.04.2018 | Interdisciplinary Research

Atoms may hum a tune from grand cosmic symphony

20.04.2018 | Physics and Astronomy

VideoLinks
Science & Research
Overview of more VideoLinks >>>