Forum for Science, Industry and Business

Sponsored by:     3M 
Search our Site:

 

A molecular switch turns on the flame in 'nature's blowtorch'

02.06.2008
New Brandeis research explains how enzyme works

Uncontrolled reaction of organic compounds with oxygen is easy: we call it fire. But nature often needs to do oxidations very specifically, adding oxygen to a particular carbon atom in a complicated molecule without disturbing anything else. Usually, this job falls to an enzyme called cytochrome P450.

Because cytochrome P450 can catalyze molecular oxidations with pinpoint accuracy, it has been called "nature's blowtorch," and its job is analogous to that of a welder doing a tricky repair in a highly flammable wooden house. It needs to do the repair without burning itself or the house.

Brandeis University researchers have been trying to understand the details of how P450 does this job so efficiently; that is, "burning" the right places in the target molecule (substrate) while not "burning down the house."

... more about:
»Molecular »Oxidation »P450 »Substrate

In new research online in the Cell Press journal Structure, chemistry graduate student Bo OuYang, along with fellow grad student Marina Dang and advisors Thomas and Susan Pochapsky, describe a new insight into how P450 works. The researchers discovered that the protein chain in P450 can change its structure by a 180 degree rotation around a single peptide bond. In one orientation, both oxygen and the molecule to be oxidized (substrate) can get in and out of the P450 active site, but the oxygen is not "activated," that is, it is not in a state to react with the substrate (or anything else, for that matter).

In the other orientation, however, the substrate is held tightly in the correct orientation for the oxidation, and the oxygen can be activated to do "the burn." The activated form of the molecule is generated by binding a helper protein, called Pdx, to the P450. This binding drives the reorientation around the peptide bond, and moves the P450 from the form in which substrate binds to the active form. After the reaction is finished, the Pdx falls off, the P450 moves back to the unactivated state, and the oxidized products are free to leave.

After another substrate molecule and oxygen move into the active site, the cycle can repeat. The reorientation of a single peptide bond, an event called an "isomerization," thus acts as a molecular switch, moving the P450 between safe and active forms while protecting the P450 and its environment from accidental oxidative damage.

Laura Gardner | EurekAlert!
Further information:
http://www.brandeis.edu

Further reports about: Molecular Oxidation P450 Substrate

More articles from Life Sciences:

nachricht Enduring cold temperatures alters fat cell epigenetics
19.04.2018 | University of Tokyo

nachricht Full of hot air and proud of it
18.04.2018 | University of Pittsburgh

All articles from Life Sciences >>>

The most recent press releases about innovation >>>

Die letzten 5 Focus-News des innovations-reports im Überblick:

Im Focus: Writing and deleting magnets with lasers

Study published in the journal ACS Applied Materials & Interfaces is the outcome of an international effort that included teams from Dresden and Berlin in Germany, and the US.

Scientists at the Helmholtz-Zentrum Dresden-Rossendorf (HZDR) together with colleagues from the Helmholtz-Zentrum Berlin (HZB) and the University of Virginia...

Im Focus: Gamma-ray flashes from plasma filaments

Novel highly efficient and brilliant gamma-ray source: Based on model calculations, physicists of the Max PIanck Institute for Nuclear Physics in Heidelberg propose a novel method for an efficient high-brilliance gamma-ray source. A giant collimated gamma-ray pulse is generated from the interaction of a dense ultra-relativistic electron beam with a thin solid conductor. Energetic gamma-rays are copiously produced as the electron beam splits into filaments while propagating across the conductor. The resulting gamma-ray energy and flux enable novel experiments in nuclear and fundamental physics.

The typical wavelength of light interacting with an object of the microcosm scales with the size of this object. For atoms, this ranges from visible light to...

Im Focus: Basel researchers succeed in cultivating cartilage from stem cells

Stable joint cartilage can be produced from adult stem cells originating from bone marrow. This is made possible by inducing specific molecular processes occurring during embryonic cartilage formation, as researchers from the University and University Hospital of Basel report in the scientific journal PNAS.

Certain mesenchymal stem/stromal cells from the bone marrow of adults are considered extremely promising for skeletal tissue regeneration. These adult stem...

Im Focus: Like a wedge in a hinge

Researchers lay groundwork to tailor drugs for new targets in cancer therapy

In the fight against cancer, scientists are developing new drugs to hit tumor cells at so far unused weak points. Such a “sore spot” is the protein complex...

Im Focus: The Future of Ultrafast Solid-State Physics

In an article that appears in the journal “Review of Modern Physics”, researchers at the Laboratory for Attosecond Physics (LAP) assess the current state of the field of ultrafast physics and consider its implications for future technologies.

Physicists can now control light in both time and space with hitherto unimagined precision. This is particularly true for the ability to generate ultrashort...

All Focus news of the innovation-report >>>

Anzeige

Anzeige

VideoLinks
Industry & Economy
Event News

Invitation to the upcoming "Current Topics in Bioinformatics: Big Data in Genomics and Medicine"

13.04.2018 | Event News

Unique scope of UV LED technologies and applications presented in Berlin: ICULTA-2018

12.04.2018 | Event News

IWOLIA: A conference bringing together German Industrie 4.0 and French Industrie du Futur

09.04.2018 | Event News

 
Latest News

Diamond-like carbon is formed differently to what was believed -- machine learning enables development of new model

19.04.2018 | Materials Sciences

Electromagnetic wizardry: Wireless power transfer enhanced by backward signal

19.04.2018 | Physics and Astronomy

Ultrafast electron oscillation and dephasing monitored by attosecond light source

19.04.2018 | Physics and Astronomy

VideoLinks
Science & Research
Overview of more VideoLinks >>>