An investigation of the genes that govern spore formation in the bacteria B. subtilis shows that chance plays a significant role in determining which of the microbes sacrifice themselves for the colony and which go on to form spores.
B. subtilis, a common soil bacteria, is a well-known survivor. When running short of food, it can alternatively band together in colonies or encase itself in a tough, protective spore to wait for better times. In fact, B. subtilis is so good at making spores that it's often used as a model organism by biologists who study bacterial spore formation.
"It's too early to say whether B. subtilis is truly altruistic," said co-author Oleg Igoshin, assistant professor of bioengineering at Rice University. "What is clear from this is that not all bacteria are going to look and act the same, and that's something that can be overlooked when people either study or attempt to control bacteria with population-wide approaches."
For example, Igoshin said doctors and food safety engineers might need to amend general approaches aimed at controlling bacteria with more targeted methods that also account for the uncharacteristic individual.
The new results appear in the April 15 issue of Molecular Systems Biology. The experimental work, which was done by Jan-Willem Veening, currently at Newcastle University, and by other members of Oscar Kuipers' research group at the University of Groningen in the Netherlands, focused on the B. subtilis genes that regulate both spore formation and the production cycles of two proteins -- subtilisin and bacillopeptidase. These two proteins help break apart dead cells and convert them into food. They are produced and released into the surrounding environment by B. subtilis cells that are running low on food.
From previous studies, scientists know there is some overlap between genes that control the production of the two proteins and those that control spore formation.
"Only a portion of the bacteria in a colony will form spores and only portion of the bacteria produce subtilisin, and we were interested in probing the genetic basis for this," Igoshin said. "How is it decided which cells become spores and which don't?"
Igoshin, a computational biologist, used computer simulations to help decipher and interpret the team's experimental results. He said the team found that fewer than 30 percent of individuals in a colony produce large quantities of the food-converting proteins. Even though the proteins benefit all members of the colony and help some cells to become spores, the cells that produce the proteins in bulk do not form spores themselves.
"There's a feedback loop, so that cells that start producing the proteins early get a reinforced signal to keep making them," Igoshin said. "We found that it's probabilistic events -- chance, if you will -- that dictates who is early and who is late. The early ones start working for the benefit of everyone while the later ones save valuable resources to ensure successful completion of sporulation program. Many cells will end up committing to sporulation before they had a chance to contribute to protease production"
Igoshin said a key piece of evidence confirming modeling predictions came in experiments that tracked genetically identical sister cells, some of which became protein producers and some of which didn't.
Jade Boyd | EurekAlert!
Warming ponds could accelerate climate change
21.02.2017 | University of Exeter
An alternative to opioids? Compound from marine snail is potent pain reliever
21.02.2017 | University of Utah
In the field of nanoscience, an international team of physicists with participants from Konstanz has achieved a breakthrough in understanding heat transport
Cells need to repair damaged DNA in our genes to prevent the development of cancer and other diseases. Our cells therefore activate and send “repair-proteins”...
The Fraunhofer IWS Dresden and Technische Universität Dresden inaugurated their jointly operated Center for Additive Manufacturing Dresden (AMCD) with a festive ceremony on February 7, 2017. Scientists from various disciplines perform research on materials, additive manufacturing processes and innovative technologies, which build up components in a layer by layer process. This technology opens up new horizons for component design and combinations of functions. For example during fabrication, electrical conductors and sensors are already able to be additively manufactured into components. They provide information about stress conditions of a product during operation.
The 3D-printing technology, or additive manufacturing as it is often called, has long made the step out of scientific research laboratories into industrial...
Nature does amazing things with limited design materials. Grass, for example, can support its own weight, resist strong wind loads, and recover after being...
Nanometer-scale magnetic perforated grids could create new possibilities for computing. Together with international colleagues, scientists from the Helmholtz Zentrum Dresden-Rossendorf (HZDR) have shown how a cobalt grid can be reliably programmed at room temperature. In addition they discovered that for every hole ("antidot") three magnetic states can be configured. The results have been published in the journal "Scientific Reports".
Physicist Dr. Rantej Bali from the HZDR, together with scientists from Singapore and Australia, designed a special grid structure in a thin layer of cobalt in...
13.02.2017 | Event News
10.02.2017 | Event News
09.02.2017 | Event News
21.02.2017 | Earth Sciences
21.02.2017 | Medical Engineering
21.02.2017 | Trade Fair News