Darwin's tree of life represents the path and estimates the time evolution took to get to the current diversity of life. Now, new findings suggest that this tree, an icon of evolution, may need to be redrawn. In research to be published in the April 13 advance online issue of Nature, researchers at Rockefeller University and the University of Tokyo have joined forces to reveal that insects have adopted a strategy to detect odors that is radically different from those of other organisms -- an unexpected and controversial finding that may dissolve a dominant ideology in the field.
Since 1991, researchers assumed that all vertebrates and invertebrates smell odors by using a complicated biological apparatus much like a Rube Goldberg device. For instance, someone pushing a doorbell would set off a series of elaborate, somewhat wacky, steps that culminate in the rather simple task of opening the door.
In the case of an insect's ability to smell, researchers believed that when molecules wafting in the air travel up the insect's nose, they latch onto a large protein (called a G-protein coupled odorant receptor) on the surface of the cell and set off a chain of similarly elaborate steps to open a molecular gate nearby, signaling the brain that an odor is present.
"It's that way in the nematode, it's that way in mammals, it's that way in every known vertebrate," says study co-author Leslie Vosshall, head of the Laboratory of Neurogenetics and Behavior at Rockefeller University. "So it's actually unreasonable to think that insects use a different strategy to detect odors. But here, we show that insects have gotten rid of all this stuff in the middle and activate the 'gate' directly."
The gate, a doughnut-shaped protein called an ion channel, provides a safe pathway for ions to flow into a cell. When molecules bind to the odor-sensitive ion channel, the protein changes its shape much like a gate or door changes its conformation as it is opened and closed. Opened, it allows millions of ions to surge into the cell. Closed, it prohibits the activity of the ions from sending a signal to the brain that an odor is present.
At the University of Tokyo, Vosshall's colleague Kazushige Touhara and his lab members puffed molecules onto cells engineered to make insect olfactory receptors. They then measured how long it took for the ion channel to open and recorded their electrical movement as they surged inside the cell via the channel. The rush of electrical activity occurred too fast for a series of steps to be involved, says Vosshall. In addition, poisoning several proteins involved in the G-protein pathway didn't affect the ions or the ion channel, suggesting that G-protein signaling isn't primarily involved in insect smell.
Experiment after experiment, "the most consistent interpretation is that these are ion channels directly gated by odors," says Vosshall. "But the dominant thinking in the field may have reflected an experimental bias that aimed at proving a more elaborate scheme."
The ion channels don't resemble any known ion channel on Earth, says Vosshall. They are composed of two proteins that work in tandem with one another: an olfactory receptor and its coreceptor, Or83b. While the coreceptor is common to every ion channel, the olfactory receptor is unique. Together, they form the olfactory receptor complex. Vosshall and Touhara specifically show that this complex forms nonselective cation channels, meaning that they allow any ion to pass through the gate as long as it has a positive charge.
Touhara and Vosshall developed their ion channel hypothesis in parallel with Vosshall's work on DEET, a widely used chemical in bug spray that jams the receptor complex. This research, which was published in Science last month, also showed that DEET jams other proteins that have nothing to do with smell, including several different types of ion channels that play important roles in the human nervous system.
What these radically different proteins have in common, though, is that they all specifically inhibit the influx of positively charged ions into the cell. "Now the curious result in the DEET paper showing that this insect repellent blocks insect olfactory receptors and unrelated ion channels makes sense," says Vosshall. "I am optimistic that we can come up with blockers specific for this very strange family of insect olfactory ion channels."
Thania Benios | EurekAlert!
ADP-ribosylation on the right track
26.04.2018 | Max-Planck-Institut für Biologie des Alterns
Flavins keep a handy helper in their pocket
25.04.2018 | University of Freiburg
Magnetic resonance imaging, or MRI, is a widely used medical tool for taking pictures of the insides of our body. One way to make MRI scans easier to read is...
At the Hannover Messe 2018, the Bundesanstalt für Materialforschung und-prüfung (BAM) will show how, in the future, astronauts could produce their own tools or spare parts in zero gravity using 3D printing. This will reduce, weight and transport costs for space missions. Visitors can experience the innovative additive manufacturing process live at the fair.
Powder-based additive manufacturing in zero gravity is the name of the project in which a component is produced by applying metallic powder layers and then...
Physicists at the Laboratory for Attosecond Physics, which is jointly run by Ludwig-Maximilians-Universität and the Max Planck Institute of Quantum Optics, have developed a high-power laser system that generates ultrashort pulses of light covering a large share of the mid-infrared spectrum. The researchers envisage a wide range of applications for the technology – in the early diagnosis of cancer, for instance.
Molecules are the building blocks of life. Like all other organisms, we are made of them. They control our biorhythm, and they can also reflect our state of...
University of Connecticut researchers have created a biodegradable composite made of silk fibers that can be used to repair broken load-bearing bones without the complications sometimes presented by other materials.
Repairing major load-bearing bones such as those in the leg can be a long and uncomfortable process.
Study published in the journal ACS Applied Materials & Interfaces is the outcome of an international effort that included teams from Dresden and Berlin in Germany, and the US.
Scientists at the Helmholtz-Zentrum Dresden-Rossendorf (HZDR) together with colleagues from the Helmholtz-Zentrum Berlin (HZB) and the University of Virginia...
13.04.2018 | Event News
12.04.2018 | Event News
09.04.2018 | Event News
26.04.2018 | Medical Engineering
26.04.2018 | Power and Electrical Engineering
26.04.2018 | Information Technology