“We’re excited to have stumbled across another family of proteins that can tell a growing nerve which way to grow,” says David Ginty, Ph.D., a professor of neuroscience at Hopkins and investigator of the Howard Hughes Medical Institute. “But the really interesting thing is that the nerves appear to use blood vessels as guideposts to direct their growth in one of several possible directions.”
The research team studied in mice a group of about 15,000 nerve cells known as the superior cervical ganglia, or SCG, which extend projections that innervate various structures in the head including the eyes, mouth and salivary glands. The SCG sits in a Y-like branching point of the blood vessel in the neck that supplies the head with blood, the carotid artery. In the developing embryo, nerve projections grow out of the SCG and grow along one of the two branches of the carotid artery; the nerves that grow along the internal carotid innervate the eyes and mouth among other head structures, and those that grow along the external carotid innervate the salivary glands.
To figure out how nerve cells “choose” to grow along the external carotid artery to innervate the salivary glands, the team looked for genes that appear to be preferentially turned on in the external carotid, and off in the internal carotid. Says Ginty, “There’s only two directions they can go and we wanted to know if they choose their direction or if the decision to go one way or the other is random.”
They found one gene that is expressed preferentially in the external carotid, a gene that makes the blood pressure regulating protein, endothelin, active. “It comes as no surprise that something critical for regulating the cardiovascular system in the adult also is used for directing nerve growth in the developing embryo,” says Ginty. “The genome is limited and nature has figured out a way to use things over and over again for unrelated functions.”
Further examination of the arteries in mouse embryos confirmed that endothelin is found only in the external carotid. To confirm that the nerve cell projections grow toward endothelin, the researchers removed SCGs and grew each one next to an endothelin-soaked bead. Checking on them three days later, the team found that nerves from the SCGs had grown towards the beads. To be certain that endothelin directs nerve growth in the living animal, the researchers then looked in mice that had the endothelin gene removed. Sure enough, these mice had no nerves growing along their external carotid arteries.
The team then wondered if all growing nerves in the SCG can respond to endothelin. So they looked for the endothelin receptors in SCG nerves and found only a subset of SCG nerves make endothelin receptors and concluded that those nerves somehow already had been chosen to respond to the endothelin made by the external carotid.
“How do these nerve cells know which target organ they’re supposed to innervate when they all come from the same progenitor?” asks Ginty. “This is what we’re going to study next.”
Individual Receptors Caught at Work
19.10.2017 | Julius-Maximilians-Universität Würzburg
Rapid environmental change makes species more vulnerable to extinction
19.10.2017 | Universität Zürich
University of Maryland researchers contribute to historic detection of gravitational waves and light created by event
On August 17, 2017, at 12:41:04 UTC, scientists made the first direct observation of a merger between two neutron stars--the dense, collapsed cores that remain...
Seven new papers describe the first-ever detection of light from a gravitational wave source. The event, caused by two neutron stars colliding and merging together, was dubbed GW170817 because it sent ripples through space-time that reached Earth on 2017 August 17. Around the world, hundreds of excited astronomers mobilized quickly and were able to observe the event using numerous telescopes, providing a wealth of new data.
Previous detections of gravitational waves have all involved the merger of two black holes, a feat that won the 2017 Nobel Prize in Physics earlier this month....
Material defects in end products can quickly result in failures in many areas of industry, and have a massive impact on the safe use of their products. This is why, in the field of quality assurance, intelligent, nondestructive sensor systems play a key role. They allow testing components and parts in a rapid and cost-efficient manner without destroying the actual product or changing its surface. Experts from the Fraunhofer IZFP in Saarbrücken will be presenting two exhibits at the Blechexpo in Stuttgart from 7–10 November 2017 that allow fast, reliable, and automated characterization of materials and detection of defects (Hall 5, Booth 5306).
When quality testing uses time-consuming destructive test methods, it can result in enormous costs due to damaging or destroying the products. And given that...
Using a new cooling technique MPQ scientists succeed at observing collisions in a dense beam of cold and slow dipolar molecules.
How do chemical reactions proceed at extremely low temperatures? The answer requires the investigation of molecular samples that are cold, dense, and slow at...
Scientists from the Max Planck Institute of Quantum Optics, using high precision laser spectroscopy of atomic hydrogen, confirm the surprisingly small value of the proton radius determined from muonic hydrogen.
It was one of the breakthroughs of the year 2010: Laser spectroscopy of muonic hydrogen resulted in a value for the proton charge radius that was significantly...
17.10.2017 | Event News
10.10.2017 | Event News
10.10.2017 | Event News
19.10.2017 | Materials Sciences
19.10.2017 | Materials Sciences
19.10.2017 | Physics and Astronomy