Forum for Science, Industry and Business

Sponsored by:     3M 
Search our Site:

 

"Exciting new approach" for identifying microRNAs

09.04.2008
PhD student at MDC develops new computer program

MicroRNAs (miRNAs) are genes which produce important elements that regulate a wide variety of processes in plants, animals and humans. MiRNAs are considered to be promising diagnostic and therapeutic candidates for the treatment of human diseases. Worldwide, scientists are seeking to develop methods to detect which miRNAs are active in tissue samples or to identify novel miRNA genes.

To date, researchers have identified more than 600 human miRNAs, each of which regulates the activity of several hundred proteins, the building and operating materials of life. Marc Friedländer, a PhD student in the laboratory of Nikolaus Rajewsky at the Max Delbrück Center for Molecular Medicine (MDC) Berlin-Buch, Germany, has now developed a software package named miRDeep. Using it, researchers can detect not only which miRNAs are active in a tissue sample, but can also discover previously unknown miRNAs. MiRDeep is based on the analysis of modern high-throughput sequencing technologies and modeling the activity of a key enzyme in the miRNA pathway.

The paper, written in collaboration with Wei Chen of the Max Planck Institute (MPI) for Molecular Genetics, Berlin, has been published in Nature Biotechnology* online (Vol. 26, No. 4, pp. 407 - 415, 008). It also reports more than 250 novel or unannotated miRNA genes, 15 of these are human, which Friedländer and his colleagues were able to identify.

... more about:
»Molecular »RNA »Rajewsky »miRNA »produce »proteins

RNA is an abbreviation for ribonucleic acid. It is a chemical relative of DNA and functions as carrier of genetic information, which the cell needs to produce proteins. Besides this messenger RNA there are also miRNAs, small RNA fragments, which bind to certain regions of messenger RNA and thus block the production of proteins. MiRNA genes thus regulate which proteins the body generates.

Researchers want to utilize this process. "For instance, cancer researchers compare cancer cells with healthy cells to find out which miRNAs might play a role in the development of cancer," Rajewsky said, explaining the significance of miRNAs for basic medical research. "However, many known, but also still unknown miRNAs can only be found in small numbers in cells and are thus overlooked using traditional methods," he added. With novel "deep sequencing" methods, researchers can detect even these miRNAs. Using these revolutionary high-throughput sequencing technologies, genetic material can be decoded more rapidly and at lower cost.

Free access for researchers
"Until now," Rajewsky explained, "the problem also involved analyzing the immense amount of data generated by deep sequencing. Such a machine can easily decode 100 million letters of DNA in 3.5 days. Moreover, cells produce many other RNAs, not only miRNAs." Marc Friedländer developed the computer program "miRDeep".

Using this program, researchers can discover signatures in the sequencing data which are generated in the production of miRNAs in the cell. MiRDeep searches the data for these traces and then computes the probability with which a potential precursor-miRNA will produce a real miRNA. MiRDeep can be downloaded as software package from the website of the Rajewsky research group.

"Due to the good collaboration of bioinformaticians and lab biologists, we have succeeded in testing miRDeep in practice," Rajewsky said, describing the work of his research team. MDC researchers tested the new program by sequencing even small RNAs of human cancer cells and blood cells in the dog and analyzing these with miRDeep. They detected most of the already known miRNAs, but also 230 miRNAs that were previously unknown.

Various new miRNA genes could then also be validated by the researchers independently in the lab. "We started very early with the analysis of deep sequencing data and were thus able to gain experience, which is necessary considering the complexity and magnitude of the data." He summed up by saying, "Generally, until now there have been very few published methods for analyzing this data. Right now we are just at the beginning of this exciting research."

*miRDeep: Discovering miRNAs from deep sequencing data

Marc R. Friedländer1, Wei Chen2, Catherine Adamidi1 , Jonas Maaskola1, Ralf Einspanier3, Signe Knespel1, and Nikolaus Rajewsky1,*

1 Max Delbrück Centrum für Molekulare Medizin, Robert-Rössle-Strasse 10, D-13125 Berlin-Buch, Germany
2 Department of Human Molecular Genetics, Max Planck Institute for Molecular Genetics, Ihnestrasse 73, D-14195 Berlin, Germany
3 Institute of Veterinary Biochemistry, Freie Universität Berlin, Oertzenweg 19b, D-14163 Berlin, Germany

doi:10.1038/nbt1394

Barbara Bachtler
Press and Public Affairs
Max Delbrück Center for Molecular Medicine (MDC) Berlin-Buch
Robert-Rössle-Straße 10; 13125 Berlin; Germany
Phone: +49 (0) 30 94 06 - 38 96
Fax: +49 (0) 30 94 06 - 38 33
e-mail: presse@mdc-berlin.de
http://www.mdc-berlin.de/en/news

Barbara Bachtler, | idw
Further information:
http://en.wikipedia.org/wiki/DNA_sequencing
http://en.wikipedia.org/wiki/RNA_interference
http://www.mdc-berlin.de/en/research/research_teams/systems_biology_of_gene_regulatory_elements/index.html

Further reports about: Molecular RNA Rajewsky miRNA produce proteins

More articles from Life Sciences:

nachricht Nanoparticle Exposure Can Awaken Dormant Viruses in the Lungs
16.01.2017 | Helmholtz Zentrum München - Deutsches Forschungszentrum für Gesundheit und Umwelt

nachricht Cholera bacteria infect more effectively with a simple twist of shape
13.01.2017 | Princeton University

All articles from Life Sciences >>>

The most recent press releases about innovation >>>

Die letzten 5 Focus-News des innovations-reports im Überblick:

Im Focus: Interfacial Superconductivity: Magnetic and superconducting order revealed simultaneously

Researchers from the University of Hamburg in Germany, in collaboration with colleagues from the University of Aarhus in Denmark, have synthesized a new superconducting material by growing a few layers of an antiferromagnetic transition-metal chalcogenide on a bismuth-based topological insulator, both being non-superconducting materials.

While superconductivity and magnetism are generally believed to be mutually exclusive, surprisingly, in this new material, superconducting correlations...

Im Focus: Studying fundamental particles in materials

Laser-driving of semimetals allows creating novel quasiparticle states within condensed matter systems and switching between different states on ultrafast time scales

Studying properties of fundamental particles in condensed matter systems is a promising approach to quantum field theory. Quasiparticles offer the opportunity...

Im Focus: Designing Architecture with Solar Building Envelopes

Among the general public, solar thermal energy is currently associated with dark blue, rectangular collectors on building roofs. Technologies are needed for aesthetically high quality architecture which offer the architect more room for manoeuvre when it comes to low- and plus-energy buildings. With the “ArKol” project, researchers at Fraunhofer ISE together with partners are currently developing two façade collectors for solar thermal energy generation, which permit a high degree of design flexibility: a strip collector for opaque façade sections and a solar thermal blind for transparent sections. The current state of the two developments will be presented at the BAU 2017 trade fair.

As part of the “ArKol – development of architecturally highly integrated façade collectors with heat pipes” project, Fraunhofer ISE together with its partners...

Im Focus: How to inflate a hardened concrete shell with a weight of 80 t

At TU Wien, an alternative for resource intensive formwork for the construction of concrete domes was developed. It is now used in a test dome for the Austrian Federal Railways Infrastructure (ÖBB Infrastruktur).

Concrete shells are efficient structures, but not very resource efficient. The formwork for the construction of concrete domes alone requires a high amount of...

Im Focus: Bacterial Pac Man molecule snaps at sugar

Many pathogens use certain sugar compounds from their host to help conceal themselves against the immune system. Scientists at the University of Bonn have now, in cooperation with researchers at the University of York in the United Kingdom, analyzed the dynamics of a bacterial molecule that is involved in this process. They demonstrate that the protein grabs onto the sugar molecule with a Pac Man-like chewing motion and holds it until it can be used. Their results could help design therapeutics that could make the protein poorer at grabbing and holding and hence compromise the pathogen in the host. The study has now been published in “Biophysical Journal”.

The cells of the mouth, nose and intestinal mucosa produce large quantities of a chemical called sialic acid. Many bacteria possess a special transport system...

All Focus news of the innovation-report >>>

Anzeige

Anzeige

Event News

12V, 48V, high-voltage – trends in E/E automotive architecture

10.01.2017 | Event News

2nd Conference on Non-Textual Information on 10 and 11 May 2017 in Hannover

09.01.2017 | Event News

Nothing will happen without batteries making it happen!

05.01.2017 | Event News

 
Latest News

Water - as the underlying driver of the Earth’s carbon cycle

17.01.2017 | Earth Sciences

Interfacial Superconductivity: Magnetic and superconducting order revealed simultaneously

17.01.2017 | Materials Sciences

Smart homes will “LISTEN” to your voice

17.01.2017 | Architecture and Construction

VideoLinks
B2B-VideoLinks
More VideoLinks >>>