The paper, “Mapping Dynamic Protein Interactions in the MAP Kinase Signaling Using Live-Cell Fluorescence Fluctuation Spectroscopy and Imaging,” was posted to the Web site of Proceedings of the National Academy of Sciences (PNAS) yesterday and will appear in a future print issue of the journal.
In this work, Brian Slaughter, Ph.D., Postdoctoral Research Fellow; Joel Schwartz, Ph.D., Managing Director of the Imaging Center; and Rong Li, Ph.D., Investigator, used sophisticated biophysical techniques to perform quantitative biochemical measurements directly in live yeast cells.
“It turns out that by using three fluorescence-based analyses we could assess the movement, concentration, and state of protein hetero- and homo-oligomerization at the single cell level,” said Dr. Slaughter. “It is a significant advance to be able to apply these quantitative techniques to the model system of yeast.”
“These technical breakthroughs represent an exciting emerging direction for molecular analysis in the future,” said Dr. Li. “They will enable biological systems to be understood with precise information regarding when, where, and to what extent molecules interact with each other during important regulatory processes.”
The Rong Li lab worked closely with the Institute’s Imaging Center to perfect the application of these techniques in yeast, calling on the Center’s expertise and cutting-edge instrumentation for microscopy-based technology.
“This work demonstrates the Institute’s tremendous strength for live-cell quantitative analysis,” said Robb Krumlauf, Ph.D., Scientific Director. “I believe this and similar techniques will become increasingly important to our ability to better understand the most fundamental events in the life cycle of a cell.”
Marie Jennings | EurekAlert!
North and South Cooperation to Combat Tuberculosis
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Researchers Discover New Anti-Cancer Protein
22.03.2018 | Universität Basel
An international team of researchers has discovered a new anti-cancer protein. The protein, called LHPP, prevents the uncontrolled proliferation of cancer cells in the liver. The researchers led by Prof. Michael N. Hall from the Biozentrum, University of Basel, report in “Nature” that LHPP can also serve as a biomarker for the diagnosis and prognosis of liver cancer.
The incidence of liver cancer, also known as hepatocellular carcinoma, is steadily increasing. In the last twenty years, the number of cases has almost doubled...
In just a few weeks from now, the Chinese space station Tiangong-1 will re-enter the Earth's atmosphere where it will to a large extent burn up. It is possible that some debris will reach the Earth's surface. Tiangong-1 is orbiting the Earth uncontrolled at a speed of approx. 29,000 km/h.Currently the prognosis relating to the time of impact currently lies within a window of several days. The scientists at Fraunhofer FHR have already been monitoring Tiangong-1 for a number of weeks with their TIRA system, one of the most powerful space observation radars in the world, with a view to supporting the German Space Situational Awareness Center and the ESA with their re-entry forecasts.
Following the loss of radio contact with Tiangong-1 in 2016 and due to the low orbital height, it is now inevitable that the Chinese space station will...
Fraunhofer Institute for Organic Electronics, Electron Beam and Plasma Technology FEP, provider of research and development services for OLED lighting solutions, announces the founding of the “OLED Licht Forum” and presents latest OLED design and lighting solutions during light+building, from March 18th – 23rd, 2018 in Frankfurt a.M./Germany, at booth no. F91 in Hall 4.0.
They are united in their passion for OLED (organic light emitting diodes) lighting with all of its unique facets and application possibilities. Thus experts in...
A new scenario seeking to explain how Mars' putative oceans came and went over the last 4 billion years implies that the oceans formed several hundred million...
For the first time, an interdisciplinary team from the University of Basel has succeeded in integrating artificial organelles into the cells of live zebrafish embryos. This innovative approach using artificial organelles as cellular implants offers new potential in treating a range of diseases, as the authors report in an article published in Nature Communications.
In the cells of higher organisms, organelles such as the nucleus or mitochondria perform a range of complex functions necessary for life. In the networks of...
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