The paper, “Mapping Dynamic Protein Interactions in the MAP Kinase Signaling Using Live-Cell Fluorescence Fluctuation Spectroscopy and Imaging,” was posted to the Web site of Proceedings of the National Academy of Sciences (PNAS) yesterday and will appear in a future print issue of the journal.
In this work, Brian Slaughter, Ph.D., Postdoctoral Research Fellow; Joel Schwartz, Ph.D., Managing Director of the Imaging Center; and Rong Li, Ph.D., Investigator, used sophisticated biophysical techniques to perform quantitative biochemical measurements directly in live yeast cells.
“It turns out that by using three fluorescence-based analyses we could assess the movement, concentration, and state of protein hetero- and homo-oligomerization at the single cell level,” said Dr. Slaughter. “It is a significant advance to be able to apply these quantitative techniques to the model system of yeast.”
“These technical breakthroughs represent an exciting emerging direction for molecular analysis in the future,” said Dr. Li. “They will enable biological systems to be understood with precise information regarding when, where, and to what extent molecules interact with each other during important regulatory processes.”
The Rong Li lab worked closely with the Institute’s Imaging Center to perfect the application of these techniques in yeast, calling on the Center’s expertise and cutting-edge instrumentation for microscopy-based technology.
“This work demonstrates the Institute’s tremendous strength for live-cell quantitative analysis,” said Robb Krumlauf, Ph.D., Scientific Director. “I believe this and similar techniques will become increasingly important to our ability to better understand the most fundamental events in the life cycle of a cell.”
Marie Jennings | EurekAlert!
Cancer diagnosis: no more needles?
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The more electronics steer, accelerate and brake cars, the more important it is to protect them against cyber-attacks. That is why 15 partners from industry and academia will work together over the next three years on new approaches to IT security in self-driving cars. The joint project goes by the name Security For Connected, Autonomous Cars (SecForCARs) and has funding of €7.2 million from the German Federal Ministry of Education and Research. Infineon is leading the project.
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A research team led by physicists at the Technical University of Munich (TUM) has developed molecular nanoswitches that can be toggled between two structurally different states using an applied voltage. They can serve as the basis for a pioneering class of devices that could replace silicon-based components with organic molecules.
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At the LASYS 2018, from June 5th to 7th, the Laser Zentrum Hannover e.V. (LZH) will be showcasing processes for the laser material processing of tomorrow in hall 4 at stand 4E75. With blown bomb shells the LZH will present first results of a research project on civil security.
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There are videos on the internet that can make one marvel at technology. For example, a smartphone is casually bent around the arm or a thin-film display is rolled in all directions and with almost every diameter. From the user's point of view, this looks fantastic. From a professional point of view, however, the question arises: Is that already possible?
At Display Week 2018, scientists from the Fraunhofer Institute for Applied Polymer Research IAP will be demonstrating today’s technological possibilities and...
So-called quantum many-body scars allow quantum systems to stay out of equilibrium much longer, explaining experiment | Study published in Nature Physics
Recently, researchers from Harvard and MIT succeeded in trapping a record 53 atoms and individually controlling their quantum state, realizing what is called a...
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